Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 109715 |
| Name | oriT_101|P1  |
| Organism | Klebsiella oxytoca isolate 101 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_OW968281 (74758..74863 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_101|P1
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 6430 | GenBank | WP_004187323 |
| Name | Relaxase_LQ191_RS28465_101|P1 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 7053 | GenBank | WP_004206885 |
| Name | t4cp2_LQ191_RS27920_101|P1 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 77659..96948
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LQ191_RS28445 (AI2946V1_5542) | 72743..73054 | + | 312 | WP_004187333 | hypothetical protein | - |
| LQ191_RS28450 (AI2946V1_5543) | 73187..73723 | + | 537 | WP_004187332 | hypothetical protein | - |
| LQ191_RS28455 (AI2946V1_5545) | 74225..74620 | - | 396 | WP_019725163 | hypothetical protein | - |
| LQ191_RS28460 | 74655..75182 | + | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| LQ191_RS28465 (AI2946V1_5547) | 75169..77148 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| LQ191_RS28470 (AI2946V1_5548) | 77162..77662 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| LQ191_RS28475 (AI2946V1_5549) | 77659..78438 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| LQ191_RS28480 (AI2946V1_5550) | 78449..79612 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| LQ191_RS28485 (AI2946V1_5551) | 79602..79862 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| LQ191_RS28490 (AI2946V1_5552) | 79887..83324 | + | 3438 | WP_015586048 | LPD7 domain-containing protein | - |
| LQ191_RS28495 (AI2946V1_5553) | 83290..83802 | + | 513 | WP_011091071 | hypothetical protein | traL |
| LQ191_RS28500 (AI2946V1_5554) | 83803..84015 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| LQ191_RS28505 (AI2946V1_5555) | 83987..84397 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| LQ191_RS28510 (AI2946V1_5556) | 84465..85178 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| LQ191_RS28515 | 85187..86338 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| LQ191_RS28520 (AI2946V1_5559) | 86350..87699 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| LQ191_RS28525 (AI2946V1_5560) | 87711..88415 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| LQ191_RS28530 (AI2946V1_5561) | 88439..88969 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| LQ191_RS28535 (AI2946V1_5562) | 88986..89375 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| LQ191_RS28540 (AI2946V1_5563) | 89421..89915 | + | 495 | WP_004187480 | hypothetical protein | - |
| LQ191_RS28545 (AI2946V1_5564) | 89912..92962 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| LQ191_RS28550 (AI2946V1_5565) | 92959..94167 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| LQ191_RS28555 (AI2946V1_5566) | 94164..94760 | + | 597 | WP_015060003 | hypothetical protein | - |
| LQ191_RS28560 (AI2946V1_5567) | 94753..96948 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| LQ191_RS28565 (AI2946V1_5568) | 96950..97603 | + | 654 | WP_015060005 | hypothetical protein | - |
| LQ191_RS28570 (AI2946V1_5569) | 97684..97914 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 10150 | GenBank | NZ_OW968281 |
| Plasmid name | 101|P1 | Incompatibility group | IncFIA |
| Plasmid size | 98209 bp | Coordinate of oriT [Strand] | 74758..74863 [+] |
| Host baterium | Klebsiella oxytoca isolate 101 |
Cargo genes
| Drug resistance gene | blaOXA-48, blaTEM-1A, blaOXA-9, ant(3'')-Ia, aac(6')-Ib, blaCTX-M-15, blaOXA-1, aac(6')-Ib-cr |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |