Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 109688 |
| Name | oriT_36|P3  |
| Organism | Klebsiella oxytoca isolate 36 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_OW969662 (40950..41055 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_36|P3
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 6417 | GenBank | WP_004187323 |
| Name | Relaxase_LQ206_RS29020_36|P3 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 7045 | GenBank | WP_004206885 |
| Name | t4cp2_LQ206_RS28720_36|P3 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 43851..63140
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LQ206_RS29000 (AI2918V1_5656) | 38935..39246 | + | 312 | WP_004187333 | hypothetical protein | - |
| LQ206_RS29005 (AI2918V1_5657) | 39379..39915 | + | 537 | WP_004187332 | hypothetical protein | - |
| LQ206_RS29010 (AI2918V1_5659) | 40417..40812 | - | 396 | WP_019725163 | hypothetical protein | - |
| LQ206_RS29015 | 40847..41374 | + | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| LQ206_RS29020 (AI2918V1_5661) | 41361..43340 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| LQ206_RS29025 (AI2918V1_5662) | 43354..43854 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| LQ206_RS29030 (AI2918V1_5663) | 43851..44630 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| LQ206_RS29035 (AI2918V1_5664) | 44641..45804 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| LQ206_RS29040 (AI2918V1_5665) | 45794..46054 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| LQ206_RS29045 (AI2918V1_5666) | 46079..49516 | + | 3438 | WP_225373171 | LPD7 domain-containing protein | - |
| LQ206_RS29050 (AI2918V1_5667) | 49482..49994 | + | 513 | WP_011091071 | hypothetical protein | traL |
| LQ206_RS29055 (AI2918V1_5668) | 49995..50207 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| LQ206_RS29060 (AI2918V1_5669) | 50179..50589 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| LQ206_RS29065 (AI2918V1_5670) | 50657..51370 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| LQ206_RS29070 | 51379..52530 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| LQ206_RS29075 (AI2918V1_5673) | 52542..53891 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| LQ206_RS29080 (AI2918V1_5674) | 53903..54607 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| LQ206_RS29085 (AI2918V1_5675) | 54631..55161 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| LQ206_RS29090 (AI2918V1_5676) | 55178..55567 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| LQ206_RS29095 (AI2918V1_5677) | 55613..56107 | + | 495 | WP_004187480 | hypothetical protein | - |
| LQ206_RS29100 (AI2918V1_5678) | 56104..59154 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| LQ206_RS29105 (AI2918V1_5679) | 59151..60359 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| LQ206_RS29110 (AI2918V1_5680) | 60356..60952 | + | 597 | WP_015060003 | hypothetical protein | - |
| LQ206_RS29115 (AI2918V1_5681) | 60945..63140 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| LQ206_RS29120 (AI2918V1_5682) | 63142..63795 | + | 654 | WP_015060005 | hypothetical protein | - |
| LQ206_RS29125 (AI2918V1_5683) | 63876..64106 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 10123 | GenBank | NZ_OW969662 |
| Plasmid name | 36|P3 | Incompatibility group | IncL/M |
| Plasmid size | 64401 bp | Coordinate of oriT [Strand] | 40950..41055 [+] |
| Host baterium | Klebsiella oxytoca isolate 36 |
Cargo genes
| Drug resistance gene | blaVIM-1, mph(E), msr(E) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |