Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   109646
Name   oriT_K. quasipneumoniae 0|P2 in_silico
Organism   Klebsiella quasipneumoniae isolate 0
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OW968433 (38640..38740 [+], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      80..85, 91..96  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_K. quasipneumoniae 0|P2
TATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   6399 GenBank   WP_001749977
Name   mobF_LQ152_RS25695_K. quasipneumoniae 0|P2 insolico UniProt ID   A0A5C2CVP7
Length   1080 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1080 a.a.        Molecular weight: 120419.59 Da        Isoelectric Point: 6.5483

>WP_001749977.1 MULTISPECIES: MobF family relaxase [Gammaproteobacteria]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLSQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVP7


Auxiliary protein

ID   3396 GenBank   WP_001749975
Name   WP_001749975_K. quasipneumoniae 0|P2 insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   7028 GenBank   WP_001749976
Name   t4cp2_LQ152_RS25700_K. quasipneumoniae 0|P2 insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57679.73 Da        Isoelectric Point: 9.5788

>WP_001749976.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Gammaproteobacteria]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVSARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELRDI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 18226..38073

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ152_RS25560 (AI3010V1_4871) 14071..15504 - 1434 WP_001288432 DNA cytosine methyltransferase -
LQ152_RS25565 (AI3010V1_4872) 15603..15875 + 273 Protein_18 IS1 family transposase -
LQ152_RS25570 15886..16092 - 207 WP_001749967 hypothetical protein -
LQ152_RS25575 (AI3010V1_4873) 16097..16744 - 648 WP_015344958 restriction endonuclease -
LQ152_RS25580 (AI3010V1_4874) 16794..17027 - 234 WP_001191790 hypothetical protein -
LQ152_RS25585 (AI3010V1_4875) 17141..17455 - 315 WP_001749965 TrbM/KikA/MpfK family conjugal transfer protein -
LQ152_RS25590 17452..17796 - 345 WP_001749964 hypothetical protein -
LQ152_RS25595 17812..18183 - 372 WP_011867773 H-NS family nucleoid-associated regulatory protein -
LQ152_RS25600 (AI3010V1_4877) 18226..18960 + 735 WP_001749963 lytic transglycosylase domain-containing protein virB1
LQ152_RS25605 (AI3010V1_4878) 18969..19250 + 282 WP_000440698 transcriptional repressor KorA -
LQ152_RS25610 (AI3010V1_4879) 19260..19553 + 294 WP_001749962 hypothetical protein virB2
LQ152_RS25615 (AI3010V1_4880) 19603..19920 + 318 WP_000496058 VirB3 family type IV secretion system protein virB3
LQ152_RS25620 (AI3010V1_4881) 19920..22520 + 2601 WP_001749961 VirB4 family type IV secretion/conjugal transfer ATPase virb4
LQ152_RS25625 (AI3010V1_4882) 22538..23251 + 714 WP_001749960 type IV secretion system protein virB5
LQ152_RS25630 (AI3010V1_4883) 23259..23486 + 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
LQ152_RS25635 (AI3010V1_4884) 23502..24542 + 1041 WP_001749958 type IV secretion system protein virB6
LQ152_RS25925 24643..24771 + 129 WP_071882546 conjugal transfer protein TraN -
LQ152_RS25640 (AI3010V1_4885) 24761..25459 + 699 WP_000646594 type IV secretion system protein virB8
LQ152_RS25645 (AI3010V1_4886) 25470..26354 + 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
LQ152_RS25650 (AI3010V1_4887) 26354..27514 + 1161 WP_000101710 type IV secretion system protein VirB10 virB10
LQ152_RS25655 (AI3010V1_4888) 27556..28551 + 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
LQ152_RS25660 (AI3010V1_4889) 28551..29084 + 534 WP_000792636 phospholipase D family protein -
LQ152_RS25930 (AI3010V1_4890) 29258..29710 - 453 WP_001749956 DUF6710 family protein -
LQ152_RS25670 30021..30262 + 242 Protein_40 relaxase -
LQ152_RS25675 (AI3010V1_4892) 30294..30944 - 651 WP_000164043 tetracycline resistance transcriptional repressor TetR(A) -
LQ152_RS25680 (AI3010V1_4893) 31050..32249 + 1200 WP_012579085 tetracycline efflux MFS transporter Tet(A) -
LQ152_RS25685 (AI3010V1_4894) 32281..32934 - 654 Protein_43 EamA family transporter -
LQ152_RS25690 (AI3010V1_4895) 33129..33302 - 174 WP_001749978 hypothetical protein -
LQ152_RS25695 (AI3010V1_4896) 33302..36544 - 3243 WP_001749977 MobF family relaxase -
LQ152_RS25700 (AI3010V1_4897) 36544..38073 - 1530 WP_001749976 type IV secretion system DNA-binding domain-containing protein virb4
LQ152_RS25705 38075..38491 - 417 WP_001749975 hypothetical protein -
LQ152_RS25710 (AI3010V1_4899) 38982..39401 + 420 WP_001749974 plasmid stabilization protein StbA -
LQ152_RS25715 (AI3010V1_4900) 39410..40126 + 717 WP_001749973 StbB family protein -
LQ152_RS25720 (AI3010V1_4901) 40128..40496 + 369 WP_000414913 plasmid stabilization protein StbC -
LQ152_RS25725 (AI3010V1_4902) 40678..41022 + 345 WP_000999874 hypothetical protein -
LQ152_RS25730 (AI3010V1_4903) 41133..41453 - 321 WP_001749971 protein CcgAII -
LQ152_RS25735 (AI3010V1_4904) 41508..41687 - 180 WP_000214483 protein CcgAI -
LQ152_RS25740 (AI3010V1_4905) 42414..42923 - 510 WP_000129958 antirestriction protein ArdA -

Host bacterium

ID   10081 GenBank   NZ_OW968433
Plasmid name   K. quasipneumoniae 0|P2 Incompatibility group   IncN
Plasmid size   51757 bp Coordinate of oriT [Strand]   38640..38740 [+]
Host baterium   Klebsiella quasipneumoniae isolate 0

Cargo genes

Drug resistance gene   blaVIM-1, dfrB1, ant(3'')-Ia, catB2, qacE, sul1, tet(A)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -