Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   109645
Name   oriT_K. quasipneumoniae 0|P1 in_silico
Organism   Klebsiella quasipneumoniae isolate 0
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OW968432 (112875..112924 [+], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      33..34
Conserved sequence flanking the
  nic site    		  GGTGTGGTGA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_K. quasipneumoniae 0|P1
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   6398 GenBank   WP_074384307
Name   traI_LQ152_RS25175_K. quasipneumoniae 0|P1 insolico UniProt ID   _
Length   1753 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1753 a.a.        Molecular weight: 190909.02 Da        Isoelectric Point: 6.1572

>WP_074384307.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Klebsiella]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANRHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKEVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRLVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLVGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGQQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKKAEQTAREMAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(971-1157)

TrwC relaxase (TrwC)

(10-284)

DNA helicase TraI (TraI_C)

(1433-1555)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(577-629)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(635-714)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 89576..113476

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ152_RS25180 (AI3010V1_4800) 85454..87762 - 2309 Protein_88 type IV conjugative transfer system coupling protein TraD -
LQ152_RS25185 (AI3010V1_4801) 88122..88853 - 732 WP_004152629 conjugal transfer complement resistance protein TraT -
LQ152_RS25190 (AI3010V1_4802) 89043..89570 - 528 WP_004153030 hypothetical protein -
LQ152_RS25195 (AI3010V1_4803) 89576..92425 - 2850 WP_004178054 conjugal transfer mating-pair stabilization protein TraG traG
LQ152_RS25200 (AI3010V1_4804) 92425..93795 - 1371 WP_004178055 conjugal transfer pilus assembly protein TraH traH
LQ152_RS25205 (AI3010V1_4805) 93782..94210 - 429 WP_023292146 hypothetical protein -
LQ152_RS25210 (AI3010V1_4806) 94203..94775 - 573 WP_023292147 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
LQ152_RS25215 (AI3010V1_4807) 94747..94986 - 240 WP_023292148 type-F conjugative transfer system pilin chaperone TraQ -
LQ152_RS25220 (AI3010V1_4808) 94997..95749 - 753 WP_004152686 type-F conjugative transfer system pilin assembly protein TraF traF
LQ152_RS25225 (AI3010V1_4809) 95770..96096 - 327 WP_004152685 hypothetical protein -
LQ152_RS25920 (AI3010V1_4810) 96142..96327 - 186 WP_004152684 hypothetical protein -
LQ152_RS25230 (AI3010V1_4811) 96324..96560 - 237 WP_023292149 conjugal transfer protein TrbE -
LQ152_RS25235 (AI3010V1_4812) 96592..98547 - 1956 WP_032429306 type-F conjugative transfer system mating-pair stabilization protein TraN traN
LQ152_RS25240 (AI3010V1_4813) 98595..99233 - 639 WP_015065635 type-F conjugative transfer system pilin assembly protein TrbC trbC
LQ152_RS25245 (AI3010V1_4814) 99246..100205 - 960 WP_015065634 conjugal transfer pilus assembly protein TraU traU
LQ152_RS25250 (AI3010V1_4815) 100249..100875 - 627 WP_004152590 type-F conjugative transfer system protein TraW traW
LQ152_RS25255 (AI3010V1_4816) 100875..101264 - 390 WP_004152591 type-F conjugative transfer system protein TrbI -
LQ152_RS25260 (AI3010V1_4817) 101264..103903 - 2640 WP_004152592 type IV secretion system protein TraC virb4
LQ152_RS25265 (AI3010V1_4818) 103975..104373 - 399 WP_004153071 hypothetical protein -
LQ152_RS25270 (AI3010V1_4819) 104381..104671 - 291 WP_023292151 hypothetical protein -
LQ152_RS25275 (AI3010V1_4820) 104668..105072 - 405 WP_023292152 hypothetical protein -
LQ152_RS25280 (AI3010V1_4821) 105139..105456 - 318 WP_023292153 hypothetical protein -
LQ152_RS25285 (AI3010V1_4822) 105457..105675 - 219 WP_004171484 hypothetical protein -
LQ152_RS25290 105699..105989 - 291 WP_032429299 hypothetical protein -
LQ152_RS25295 (AI3010V1_4823) 105994..106404 - 411 WP_023292154 hypothetical protein -
LQ152_RS25300 (AI3010V1_4824) 106536..107105 - 570 WP_032429300 type IV conjugative transfer system lipoprotein TraV traV
LQ152_RS25305 107124..107339 - 216 WP_230128956 hypothetical protein -
LQ152_RS25310 (AI3010V1_4825) 107336..108760 - 1425 WP_023292156 F-type conjugal transfer pilus assembly protein TraB traB
LQ152_RS25315 (AI3010V1_4826) 108760..109500 - 741 WP_004152497 type-F conjugative transfer system secretin TraK traK
LQ152_RS25320 (AI3010V1_4827) 109487..110053 - 567 WP_020316627 type IV conjugative transfer system protein TraE traE
LQ152_RS25325 (AI3010V1_4828) 110073..110378 - 306 WP_004178059 type IV conjugative transfer system protein TraL traL
LQ152_RS25330 (AI3010V1_4829) 110392..110760 - 369 WP_004178060 type IV conjugative transfer system pilin TraA -
LQ152_RS25335 110814..111185 - 372 WP_004208838 TraY domain-containing protein -
LQ152_RS25340 (AI3010V1_4830) 111269..111964 - 696 WP_023292158 transcriptional regulator TraJ family protein -
LQ152_RS25345 (AI3010V1_4831) 112171..112563 - 393 WP_004178062 conjugal transfer relaxosome DNA-binding protein TraM -
LQ152_RS25350 (AI3010V1_4832) 113069..113476 + 408 WP_225375048 transglycosylase SLT domain-containing protein virB1
LQ152_RS25355 (AI3010V1_4833) 113514..114044 - 531 WP_230128958 antirestriction protein -
LQ152_RS25360 (AI3010V1_4834) 114669..115502 - 834 WP_038990979 N-6 DNA methylase -
LQ152_RS25365 (AI3010V1_4835) 115549..115779 - 231 WP_038990977 hypothetical protein -
LQ152_RS25370 (AI3010V1_4836) 115867..116214 - 348 WP_038990975 hypothetical protein -
LQ152_RS25375 (AI3010V1_4837) 116281..116661 - 381 WP_038990973 hypothetical protein -
LQ152_RS25380 (AI3010V1_4838) 117566..117685 - 120 WP_223156217 type I toxin-antitoxin system Hok family toxin -
LQ152_RS25385 117630..117782 - 153 WP_224230667 DUF5431 family protein -
LQ152_RS25390 (AI3010V1_4839) 117941..118267 - 327 WP_038992719 hypothetical protein -

Host bacterium

ID   10080 GenBank   NZ_OW968432
Plasmid name   K. quasipneumoniae 0|P1 Incompatibility group   IncFIB
Plasmid size   131788 bp Coordinate of oriT [Strand]   112875..112924 [+]
Host baterium   Klebsiella quasipneumoniae isolate 0

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   silE, silS, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoD, pcoR, pcoS, pcoE, arsC, arsB, arsA, arsD, arsR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -