Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   109644
Name   oriT_C. koseri 0|P2 in_silico
Organism   Citrobacter koseri isolate 0
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OW969713 (31055..31155 [+], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      32..38, 43..49  (TTACGAT..ATCGTAA)
 21..26, 38..43  (TTTTCA..TGAAAA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_C. koseri 0|P2
AATTAGAATAATTTGTTTTGTTTTCAAGCATTTACGATGAAAATCGTAATTGCGTATGGTGTATAGCCGTTAAGGGATACCATACCACGCCTTTTTTAAGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   6397 GenBank   WP_023187071
Name   mobF_LQV24_RS22615_C. koseri 0|P2 insolico UniProt ID   _
Length   969 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 969 a.a.        Molecular weight: 108499.54 Da        Isoelectric Point: 9.7610

>WP_023187071.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLNITTITRQRVDKVVDYYADGADDYYAKDGNAMQWQGAGAEELGLTGEVEQKRFAKLLDGKVTDDISVM
RKTANSESKERLGYDLTFSAPKGVTLQALVNGDKRIIEAHDRAVTKAIEEAERLALGRFTVNKKTHVENT
NKLIVGKFRHETSRELDPDLHTHAFVMNLTKTSDGKWRSLTNDGIVNSLTLLGNVYKSELARELELAGYN
LRYDRNGTFDLAHFSQEQIAQFSQRSQQIEAELAKNGLSRETASHEQKNAAALKTRKSKGEVDRDVLYEG
WKNRAKELQIDFDSREWKGAANDDIARNTQPAALEKPIEYQADKVMTFAIRSLTERQSVITERELMDVAM
KHGYGRLTLDDIRAARERAITSGNLIKEEATYAAATTNKKQQNVAMTREQWVTELVKAGRNKDEARKLVD
KGITNGRLVQQKPRFTTQLAQQRERNILKMEREGRGKIQTPYTREFSEGWLASRTLKPEQLKAVMGIIHT
PNQFISVHGFAGTGKSYMTKSAADFLKEQGVHVTSLAPYGSQVKALQAEGLESRTLQSFLRASDKKIGPG
SVVFIDEAGVIPARQMEETMRVIRDAGARAVFLGDTKQTKAVEAGKPFEQLIKAGMETAYMKDIQRQKDP
ELLKAALNAAEGKIKASLTHVTSIVEEKNHDQRYRRIVGDYVAMPPTDRANALIITGTNDSRKKINEYIQ
AELGLKGQGINYPLLNRLDTTQAQRQHSKYYEKGAVIIPERDYSNGLKRGAVYTVLDTGPGNKLTVSDGS
GDTIAFSPARFSKLSVYSVEKTELAVGDQVRITRNDAHLDLANGDRFKVKGVQSGEVLLENEKGRLVKID
ANKPMYLGLAYASTVHSAQGLTCDKVFINMDTRSLTTAKDVYYVAVTRAKHEAVIYTDDEKKLDKAASRE
GFKTAALELEQLKRYAKEQQHDAREKGRDKTEPTQEKDNSKRKGKGHDKNNDERAFTSL

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(475-649)

UvrD-like helicase C-terminal domain (UvrD_C_2)

(850-896)

TrwC relaxase (TrwC)

(14-287)


  Protein structure



No available structure.




T4CP

ID   7027 GenBank   WP_021536815
Name   t4cp2_LQV24_RS22620_C. koseri 0|P2 insolico UniProt ID   _
Length   525 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 525 a.a.        Molecular weight: 58961.81 Da        Isoelectric Point: 9.3727

>WP_021536815.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MNEADKGKICLGALLFTPLVTWFIAAKALYGFTPKDARERLVLLIQQTFDLWPLWGALLVGEIIAIIGLI
IFFKFNHQIFKGAPFKKIYRGTRLVSQRALASITKEREPQITIADIPIPTKAEGTHISIAGATGVGKSTI
FAEMMKCCLMRGKMQRVASNKDRMIILDPDGDFLSRFYKKGDKILNPYDARTEGWVFFNEIKSDFDFERF
GVSIVPTAKDSQTEEWNGYGRLLFTEVSRKVFNTSRNPTMEEVFYWTNEVPLEELEEYVRGTKAQALFAG
SGRAVGSARFVLSNKLAAHLKMPAGNFSIREWLEDPKGGNLYITWTDEMREALKSLISCWTDSIFSIVLG
MSKSNSRKIWTFIDELESLDYLPSLRDALTKGRKKGLRVVTGYQSYSQVISIYGSDVAETLLSNHRTSVV
MAAGRLGERTLEFVSKSLGEIEGEREKTGISRRFGQLGTKNTNDDHKRERAVTPTEIATLDDLTGYIAFP
GNLPVAKFETHHVNYTRSNPVPGVVLRESTAFAGM

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(111-499)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 12822..30613

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQV24_RS22470 (AN2353V1_4359) 8351..8935 - 585 WP_021536796 recombinase family protein -
LQV24_RS22475 (AN2353V1_4360) 9127..9426 + 300 WP_023187081 hypothetical protein -
LQV24_RS22480 (AN2353V1_4362) 9689..9934 + 246 WP_000195442 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
LQV24_RS22485 (AN2353V1_4363) 9934..10476 + 543 WP_023187079 hypothetical protein -
LQV24_RS22490 (AN2353V1_4364) 10446..10742 + 297 WP_230012754 hypothetical protein -
LQV24_RS22495 10732..11058 + 327 WP_000817647 endoribonuclease MazF -
LQV24_RS22500 (AN2353V1_4365) 11067..11504 + 438 WP_023187077 hypothetical protein -
LQV24_RS22935 (AN2353V1_4366) 11719..12120 - 402 WP_023187076 Hha/YmoA family nucleoid-associated regulatory protein -
LQV24_RS22510 (AN2353V1_4367) 12121..12846 - 726 WP_015345246 restriction endonuclease -
LQV24_RS22515 (AN2353V1_4368) 12822..13136 - 315 WP_000851068 hypothetical protein tfc15
LQV24_RS22520 13139..13456 - 318 WP_001226231 TrbM/KikA/MpfK family conjugal transfer protein -
LQV24_RS22525 (AN2353V1_4369) 13462..13899 - 438 WP_023187074 hypothetical protein -
LQV24_RS22530 13911..14147 - 237 WP_021536804 H-NS histone family protein -
LQV24_RS22535 (AN2353V1_4370) 14210..14950 + 741 WP_000815895 lytic transglycosylase domain-containing protein virB1
LQV24_RS22540 (AN2353V1_4371) 14973..15290 + 318 WP_001267114 hypothetical protein -
LQV24_RS22545 (AN2353V1_4372) 15274..15564 + 291 WP_021536806 TrbC/VirB2 family protein virB2
LQV24_RS22550 (AN2353V1_4373) 15616..15930 + 315 WP_001053888 VirB3 family type IV secretion system protein virB3
LQV24_RS22555 (AN2353V1_4374) 16287..18560 + 2274 WP_223939899 VirB4 family type IV secretion/conjugal transfer ATPase -
LQV24_RS22560 (AN2353V1_4375) 18570..19277 + 708 WP_023187073 type IV secretion system protein virB5
LQV24_RS22565 (AN2353V1_4376) 19281..19517 + 237 WP_000733633 EexN family lipoprotein -
LQV24_RS22570 (AN2353V1_4377) 19572..20579 + 1008 WP_170879277 type IV secretion system protein virB6
LQV24_RS22575 (AN2353V1_4378) 20674..20844 + 171 WP_021536809 hypothetical protein -
LQV24_RS22580 (AN2353V1_4379) 20847..21563 + 717 WP_000914645 type IV secretion system protein virB8
LQV24_RS22585 (AN2353V1_4380) 21566..22453 + 888 WP_021536810 TrbG/VirB9 family P-type conjugative transfer protein virB9
LQV24_RS22590 (AN2353V1_4381) 22450..23604 + 1155 WP_021536811 type IV secretion system protein VirB10 virB10
LQV24_RS22595 (AN2353V1_4382) 23597..24628 + 1032 WP_021536812 P-type DNA transfer ATPase VirB11 virB11
LQV24_RS22600 (AN2353V1_4383) 24591..25145 + 555 WP_000616063 phospholipase D family protein -
LQV24_RS22605 (AN2353V1_4384) 25146..25640 + 495 WP_023187072 GNAT family protein -
LQV24_RS22610 (AN2353V1_4385) 25652..25846 + 195 WP_000529710 hypothetical protein -
LQV24_RS22615 (AN2353V1_4386) 26124..29033 - 2910 WP_023187071 MobF family relaxase -
LQV24_RS22620 (AN2353V1_4387) 29036..30613 - 1578 WP_021536815 type IV secretion system DNA-binding domain-containing protein virb4
LQV24_RS22625 (AN2353V1_4388) 30606..30953 - 348 WP_001139329 hypothetical protein -
LQV24_RS22630 (AN2353V1_4389) 31429..31863 + 435 WP_021544362 hypothetical protein -
LQV24_RS22635 (AN2353V1_4390) 31876..32586 + 711 WP_021544361 StbB family protein -
LQV24_RS22640 (AN2353V1_4391) 32588..32956 + 369 WP_015345231 plasmid stabilization protein StbC -
LQV24_RS22645 (AN2353V1_4392) 33170..33940 + 771 WP_021544360 SprT-like domain-containing protein -
LQV24_RS22650 (AN2353V1_4393) 33981..34169 - 189 WP_001001010 hypothetical protein -
LQV24_RS22655 (AN2353V1_4394) 34174..34431 - 258 WP_021536820 hypothetical protein -
LQV24_RS22660 (AN2353V1_4395) 34504..34659 - 156 WP_021536821 hypothetical protein -
LQV24_RS22665 (AN2353V1_4396) 34811..35053 - 243 WP_023187069 hypothetical protein -
LQV24_RS22670 (AN2353V1_4397) 35107..35394 - 288 WP_001098466 hypothetical protein -

Host bacterium

ID   10079 GenBank   NZ_OW969713
Plasmid name   C. koseri 0|P2 Incompatibility group   -
Plasmid size   35534 bp Coordinate of oriT [Strand]   31055..31155 [+]
Host baterium   Citrobacter koseri isolate 0

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -