Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   109320
Name   oriT_no75|unnamed in_silico
Organism   Salmonella enterica strain no75
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP075373 (54380..54633 [+], 254 nt)
oriT length   254 nt
IRs (inverted repeats)      152..157, 159..164  (AAAAGT..ACTTTT)
 121..129, 134..142  (TTAAGGCTT..AAGCCTTAA)
 50..55, 59..64  (AATTTT..AAAATT)
Location of nic site      77..78
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 254 nt

>oriT_no75|unnamed
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGAAGAAATTTTGATAAAATTCTGGTCAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   6254 GenBank   WP_000980119
Name   mobH_KH432_RS25065_no75|unnamed insolico UniProt ID   A0A0H3VVI8
Length   1050 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1050 a.a.        Molecular weight: 118035.42 Da        Isoelectric Point: 4.6054

>WP_000980119.1 MULTISPECIES: MobH family relaxase [Enterobacteriaceae]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDAITKITDT
VETAVLKVNDLGRSTASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDATMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEEEDEEHEIIDYTDFTQLSVSRPEVG
SCATSSSVHNEKLLPEPSELPELNREQNADPQGTIERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFATTESDQNGEFSLISEGDVTELEFVEIASVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(108-304)


  Protein structure


Source ID Structure
AlphaFold DB A0A0H3VVI8


T4CP

ID   6816 GenBank   WP_046788497
Name   traD_KH432_RS25060_no75|unnamed insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78171.95 Da        Isoelectric Point: 8.0177

>WP_046788497.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANETLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKFH

  Protein domains


Predicted by InterproScan.

AAA-like domain (AAA_10)

(199-249)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(406-609)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 224406..233613

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KH432_RS25980 (KH432_25980) 224406..227087 - 2682 WP_000387412 TraC family protein virb4
KH432_RS25985 (KH432_25985) 227096..228046 - 951 WP_001022587 IncHI-type conjugal transfer lipoprotein TrhV traV
KH432_RS25990 (KH432_25990) 228056..228808 - 753 WP_183079335 protein-disulfide isomerase HtdT -
KH432_RS25995 (KH432_25995) 228927..229409 - 483 WP_000377633 hypothetical protein -
KH432_RS26000 (KH432_26000) 229417..230772 - 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
KH432_RS26005 (KH432_26005) 230762..231223 - 462 WP_000521240 plasmid transfer protein HtdO -
KH432_RS26010 (KH432_26010) 231225..232496 - 1272 WP_000592090 type-F conjugative transfer system secretin TraK traK
KH432_RS26015 (KH432_26015) 232496..233284 - 789 WP_000783153 TraE/TraK family type IV conjugative transfer system protein traE
KH432_RS26020 (KH432_26020) 233296..233613 - 318 WP_000043357 type IV conjugative transfer system protein TraL traL
KH432_RS26025 (KH432_26025) 233664..234017 - 354 WP_000423602 hypothetical protein -
KH432_RS26030 (KH432_26030) 235166..236041 - 876 WP_000594612 RepB family plasmid replication initiator protein -
KH432_RS26380 236518..236874 + 357 WP_235674127 hypothetical protein -
KH432_RS26385 236943..237572 + 630 WP_241651415 hypothetical protein -
KH432_RS26040 (KH432_26040) 237590..237790 + 201 WP_000594931 hypothetical protein -
KH432_RS26045 (KH432_26045) 237787..238503 + 717 WP_046788421 zinc finger-like domain-containing protein -

Host bacterium

ID   9755 GenBank   NZ_CP075373
Plasmid name   no75|unnamed Incompatibility group   -
Plasmid size   250232 bp Coordinate of oriT [Strand]   54380..54633 [+]
Host baterium   Salmonella enterica strain no75

Cargo genes

Drug resistance gene   oqxB, oqxA, aadA2, cmlA1, ant(3'')-Ia, sul3, dfrA12, sul1, floR, sul2, aph(4)-Ia, aac(3)-IVa, blaCTX-M-14, fosA3, mcr-1.1
Virulence gene   -
Metal resistance gene   terE, terD, terC, terB, terA, terZ, terW
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -