Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 109316
• Name: oriT_SE3.9|p5
• Organism: Staphylococcus haemolyticus strain SE3.9
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP049096 (1184..1218 [-], 35 nt)
• oriT length: 35 nt
• IRs (inverted repeats): _
• Location of nic site: 25..26
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 35 nt

>oriT_SE3.9|p5
TTACGAAGTAAATAAGTCTAGTGTGTTATACTTTA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Rep_trans_RES7_RS11710_SE3.9|p5

• ID: 6252
• GenBank: WP_029264623
• Name: Rep_trans_RES7_RS11710_SE3.9|p5
• UniProt ID: A0A2J6NKJ6
• Length: 312 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 312 a.a.
• Molecular weight: 37179.39 Da
• Isoelectric Point: 6.5219

>WP_029264623.1 MULTISPECIES: replication initiation factor domain-containing protein [Bacilli]
MIKKAEEIQAKQSLENENLNFSKTGYSNSRLSVQTLGQSQPKLSFDAMTIVGNLNKNNAQKLSEFMSVEP
QIRLWDILQTKFKAKALQEKVYIEYDKVKADTWDRRNMRVEFNPNKLTHDEMLWLKKNVIDYMEDDGFTR
LDLAFDFEDDLSDYYAMTDKTVKKTVLYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVMSEHL
WRVEVELKRDMVDYWNDCFNDLHILKPAWTTLEKINEQAMVYTLLHEESMWGKLSRNTKYKFKNLIKEIS
PVDLTELMKSTLKANEKQLQKQIDFWLSDFEF

Protein domains

Predicted by InterproScan.

• Replication initiation factor (Rep_trans) (138-285)

Protein structure

• AlphaFold DB: A0A2J6NKJ6

Host bacterium

• ID: 9751
• GenBank: NZ_CP049096
• Plasmid name: SE3.9|p5
• Incompatibility group: -
• Plasmid size: 3674 bp
• Coordinate of oriT [Strand]: 1184..1218 [-]
• Host baterium: Staphylococcus haemolyticus strain SE3.9

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -