Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 109222
• Name: oriT_pSBR2
• Organism: Staphylococcus sp. NRL 16/872
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP119329 (4773..4870 [+], 98 nt)
• oriT length: 98 nt
• IRs (inverted repeats): 49..55, 59..65 (TTGGGGA..TCCCCAA) 21..26, 33..38 (ATTTTT..AAAAAT) 23..28, 32..37 (TTTTTC..GAAAAA) 1..6, 12..17 (TGGCTA..TAGCCA)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 98 nt

>oriT_pSBR2
TGGCTAGCAATTAGCCACTTATTTTTTCGTAGAAAAATCCTAAGGGGCTTGGGGATATTCCCCAACAAGCAGGCGTCGATACCACGTGAGTGGCTAGC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_MT340_RS12660_pSBR2

• ID: 6188
• GenBank: WP_243603954
• Name: Relaxase_MT340_RS12660_pSBR2
• UniProt ID: _
• Length: 335 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 335 a.a.
• Molecular weight: 38446.22 Da
• Isoelectric Point: 6.5119

>WP_243603954.1 relaxase/mobilization nuclease domain-containing protein [Staphylococcus sp. NRL 16/872]
MATTKLSATKSTSRAINYAEKRAEEKSGLNCDIDYAKSSFKATRELYGKIGSNEGHVIIQSFKPGEVTPE
QCNQLGLALAEKFAPNHQVAVYTHNDTDHVHNHIVINSIDLETGKKFNNNKQALRDLRDFNDDICREHGL
SVPEKDTARLRYTQTEKAIADPNTKSTAQYSWKDEIREAIDQSTATNMDEFKDHLSQYGIEIARVTPKSI
TYRHLAEDKKVRGRRLGEDYNKGGIEDGFERKIQRQQQEREDDNDIQPKRPTSNRDEPAQRDTGVTQSDW
DKFAQDTNELERRRQAAESARLADEKARRDREERERAKQASRTIIDNDRDHGLEL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (9-241)

Protein structure

No available structure.

Host bacterium

• ID: 9657
• GenBank: NZ_CP119329
• Plasmid name: pSBR2
• Incompatibility group: -
• Plasmid size: 27165 bp
• Coordinate of oriT [Strand]: 4773..4870 [+]
• Host baterium: Staphylococcus sp. NRL 16/872

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21