Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 108961 |
Name | oriT_pB-4548_1 |
Organism | Salmonella enterica subsp. enterica serovar Gallinarum strain SCPM-O-B-4548 |
Sequence Completeness | - |
NCBI accession of oriT (coordinates [strand]) | NZ_CP088143 (51168..51429 [-], 262 nt) |
oriT length | 262 nt |
IRs (inverted repeats) | 92..98, 111..117 (ATTTAAT..ATTAAAT) 103..108, 111..116 (TTTAAT..ATTAAA) 31..38, 41..48 (GCAAAATT..AATTTTGC) |
Location of nic site | _ |
Conserved sequence flanking the nic site |
_ |
Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 262 nt
GTCCTCACCACCAAAAGCACCACACCCCACGCAAAATTAAAATTTTGCTGATTTGTTTTTAGTAACAGTGAGTTATGTTCATAATAATGTAATTTAATTTATTTTAATGTATTAAATAGCGGTGCAGGCACGTCGCCGGCACCGCTATGACGACGCTATACAGGGGTAGTACTGACTATTTTTATAAAAAACATTGCATTATATCAGGGGCGCTGCTAGCGTCGCTGTGCCTGTTTTTAAGTAACACCCCTGGGGGCGCTGC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 6007 | GenBank | WP_000986920 |
Name | traI_LRM93_RS22710_pB-4548_1 | UniProt ID | A0A5Y3LDY8 |
Length | 1751 a.a. | PDB ID | |
Note | Predicted by oriTfinder 2.0 |
Relaxase protein sequence
Download Length: 1751 a.a. Molecular weight: 191121.19 Da Isoelectric Point: 5.7555
MMSIAQVRSAGSAAGYYSDRDNYYVLGSMEERWAGKGAEQLGLQGAVDKEVFTRVLEGRLPDGADLSRQQ
DGGNKHRPGYDLTFSAPKSVSLMAMLAGDKRLTEAHNQAVDIAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTLHDGEWKTLSSDKVGKTGFIENIYANQIAFGKIYRAVLKEKVEA
LGYETEVVGKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPAVKMAEWMQT
LKDTGFDISAYREAADRRAEIQAAQPVPSQEQPDIQQAVTQAIAGLSDRKVQFTYTDVLARTVGMLPPEA
GVIEKARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSSDIMKQNRVTVHPEKSVPRTGSYSD
AVSVLARDRPSLAIISGQGGAAGQRERVAELTMMAREQGREVQIIAADRRSQTNLKQDERLSGELITGRR
QLQEGMTFSPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKEAGVNSYRWQ
GGQQTPATVISEPDRNVRYARLAGDFVAAVKTGEESVAQVSGVREQAILAGMIRSELKTQGILGQQDTMM
TALSPVWLDSRNRYLRDMYREGMVMEQWNPEKRSHDRYVIDRVTAQSHSLTLRDAQGGTQMVRISALDSS
WSLFRPEKIPVADGERLMVTGKIPWLRVSGGDRLQVSAVNDGVMTVTVPGRAEPASLPVGDSPFTALKLE
NGWVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDEIRTAEKLSRHPSFTVVSEQIK
ARAGEVSLETAISLQKAGLHTPAQQALHLAIPVVESKNLAFSQVELLTEAKSFAAEGTGFADLGREIDAQ
IKRGDLLYVDVAKCYGTDLLISRASYDAEKSILHHILEGKEAVTPLMERVPGELMETLTSGQRAATRMIL
ETKDRFTVVQGYAGVGKTTQFRAVMSAVNLLPEDERPRVVGLGPTHRAVGEMRSAGVEAQTLASFLHDTQ
LQQRSGEAPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQARSAA
DVAIMKEIVRQTPELRDAVYSLINRDVNKALSGLENVKPVQVPRLKGAWAPENSVTEFSRLQERELAKAA
QEAEKKGEAFPDVPVTLYEAIVLDYTGRMPEAREQTLIVTHLNEDRRVLNSMIHDVREKAGELGEQQADI
PVLTTANIRDGELRRLSTWEAHPGALALVDNVYHRIAGISKEDGLITLEDKAGNTRLISPREAAAEGVTL
YNPETIRVGAGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGKQTCVVRPGQDRAEQHIDLAYAIT
AHGAQGASETFAIALEGTEGGRKQMAGFESAYVALSRMKQHVQVYTDDRQGWVKAINSAEQKGTAHDVLE
PKSEREMMNAERLFSTARELRDVAVGRAVLRNAGLAQGDSRARFIAPGRKYPQPYVALPAFDRNGKSAGI
WLNPLTTDDGAGLRGFTGEGRVKGSEEAQFVALQGSRNGESLLAGSMQDGVRIARENPDSGVVVRIAGDG
RPWNPVAITGGRVWGDIPDSNVQPGAGNGEPVTAEILAQRQAEEAVRRETERRADEIVRKMAEDKSDLPE
EKTAQAVREIAGQEQDRMTPPEREPALPESVLREPQRERETIREVARENRGRERLQEMVCDLQKEKTPGG
D
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
ID | 3039 | GenBank | WP_001676655 |
Name | WP_001676655_pB-4548_1 | UniProt ID | H3JT82 |
Length | 75 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
Auxiliary protein sequence
Download Length: 75 a.a. Molecular weight: 9147.26 Da Isoelectric Point: 9.6183
MRRRNAREGISRTVSVYLDEDTNNRLIRAKDRSGRSKTIEVQIRLRDHLKRYPDFYNEEIFREVIEETES
TFKEI
Protein domains
Predicted by InterproScan.
Protein structure
Source | ID | Structure |
---|---|---|
AlphaFold DB | H3JT82 |
ID | 3040 | GenBank | WP_001151546 |
Name | WP_001151546_pB-4548_1 | UniProt ID | Q93GP1 |
Length | 126 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
Auxiliary protein sequence
Download Length: 126 a.a. Molecular weight: 14366.30 Da Isoelectric Point: 5.7236
MAKVNVYISNEVHNKITAIVEKRRQEGARDKDISFSGTSSMLLELGLRVYEAQMERKESPFNQTEFNKVL
LENVLKTQSSVAKILGIGSLSPHVAGNPKFEYANMVEDIKEKVSSEMERFFHENEE
Protein domains
Predicted by InterproScan.
Protein structure
Source | ID | Structure |
---|---|---|
AlphaFold DB | Q93GP1 |
T4CP
ID | 6497 | GenBank | WP_000009313 |
Name | traD_LRM93_RS22725_pB-4548_1 | UniProt ID | _ |
Length | 744 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 744 a.a. Molecular weight: 84338.18 Da Isoelectric Point: 5.2353
MSFNAKDMTQGGQIASMRIRMFSQIANIIFYCLFIFFWILVGLVLWVKLIWQTFVNGCIYWWCTTLEGMR
DLIKSQPVYEIRYYGQTFRMNAAQVLQDKYTVWCGEQLWSAFVLAACVALVICLITFFMVTWILGRQGKQ
QSEDDVTGGHQLTDNPKDVARMLKKDGKASDILIGDLPIIKDSEIQNFLLHGTVSTGKSEIIRRLANYAR
QRGDMVVMYDRSCEFVKSYYDPSIDKILNPCDARCAAWDLWRECLTLPDFDNAANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDSDRSYSKLVDTLLSIKIEKLRTYLRDSPAANLVEEKIEKTAISIRAVLTNYVKA
VRYLQGIERNGEPFTIRDWMRGVREDQKNGWLFISSNADTHSSLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEIVPEARKFGGCYVFGIQSSTQMEDIYGEKAAATLLDVMNTRAFFRSPSYKIA
DYVAHEIGEKEILKASEQYSYGADPVRDGVSTGKEMERVTLVSYSDIQSLPDLTCYVTLPGPYPAVKLAL
KYQERPKVAPEFIPREMNPEAEARLSAVLAAREAEGRQMSSLFEPDTEAVAPAAAEAQAEQPQQPQQPQQ
PQQPQQPQQPQQPQQPQQPQSTVVSDKKSAAAGTAVNTPAGGVGQELKMKQEDEEQPLPPGINASGEVVD
MAVYERWRQEQELNTQQQMQRREEVNINVHRGHGKDEPEPGDHF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
ID | 6498 | GenBank | WP_000793311 |
Name | traC_LRM93_RS22820_pB-4548_1 | UniProt ID | _ |
Length | 882 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 882 a.a. Molecular weight: 100401.28 Da Isoelectric Point: 6.8187
MKNLLDTVTQAANSLLTALKMPDESAQANQILGEMSFPQFSRLLPYRDYNQESGLFMNDATMGFMLEATP
VNGANETIALSLDHLLRTKLPRGIPLCVHLMSSQLVGERIEYGLREFSWSGEQAERLNAITRAYYMKAAE
THFPLPEGMNLPLTLRHYRVFISYCAPSRKKSRADILEMENLVKIIRASLQGAYISTQSVDAKAFINIVG
EMINHNPESLHPTRRRLDPYSDLNYQCVDDSFDLKVRQDYLTLGLRDNGKNSTARIMSFHLARNPEIAFL
WNSADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGD
LRQRLASNQSSVVSYFFNITAFCRDNKEMALETEQDILNSFRKNGFELISPRFNHMRNFLACLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITNIDQSAERIRDQLSV
MASPNGNLDEVHEGLLLQAVRAAWLTKKNRARIDDVVYFLENARTSEQYAESPGIRSRLDEMIVLLNQYT
AAGTYGRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGWR
LLDFKNRKVGDFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLYPDQFPQLHREMIEKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGL
SIHEAVWQLAWKKSGPEMASLEAWLQEHETFRGTYEYKAETD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 24106..51970
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
LRM93_RS22715 (LRM93_22715) | 23472..23699 | + | 228 | WP_000450263 | toxin-antitoxin system antitoxin VapB | - |
LRM93_RS22720 (LRM93_22720) | 23699..24097 | + | 399 | WP_000911323 | tRNA(fMet)-specific endonuclease VapC | - |
LRM93_RS22725 (LRM93_22725) | 24106..26340 | - | 2235 | WP_000009313 | type IV conjugative transfer system coupling protein TraD | prgC |
LRM93_RS22730 (LRM93_22730) | 26620..27231 | - | 612 | WP_232520941 | hypothetical protein | - |
LRM93_RS22735 (LRM93_22735) | 27351..28082 | - | 732 | WP_000782437 | conjugal transfer complement resistance protein TraT | - |
LRM93_RS22740 (LRM93_22740) | 28181..28492 | + | 312 | WP_000522257 | hypothetical protein | - |
LRM93_RS22745 (LRM93_22745) | 28495..31314 | - | 2820 | Protein_30 | conjugal transfer mating-pair stabilization protein TraG | - |
LRM93_RS22750 (LRM93_22750) | 31311..32693 | - | 1383 | WP_001161718 | conjugal transfer pilus assembly protein TraH | traH |
LRM93_RS22755 (LRM93_22755) | 32683..33231 | - | 549 | WP_000059056 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
LRM93_RS22760 (LRM93_22760) | 33218..33511 | - | 294 | WP_001233880 | type-F conjugative transfer system pilin chaperone TraQ | - |
LRM93_RS22765 (LRM93_22765) | 33887..34231 | - | 345 | WP_001697170 | conjugal transfer protein TrbA | - |
LRM93_RS22770 (LRM93_22770) | 34246..34995 | - | 750 | WP_001226834 | type-F conjugative transfer system pilin assembly protein TraF | traF |
LRM93_RS22775 (LRM93_22775) | 35008..35223 | - | 216 | WP_000783297 | conjugal transfer protein TrbE | - |
LRM93_RS22780 (LRM93_22780) | 35233..35709 | - | 477 | WP_000046068 | hypothetical protein | - |
LRM93_RS22785 (LRM93_22785) | 35720..37597 | - | 1878 | WP_000730206 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
LRM93_RS22790 (LRM93_22790) | 37799..37936 | + | 138 | WP_154071808 | hypothetical protein | - |
LRM93_RS22795 (LRM93_22795) | 37957..38586 | - | 630 | WP_000089759 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
LRM93_RS22800 (LRM93_22800) | 38583..38867 | - | 285 | WP_232520943 | hypothetical protein | - |
LRM93_RS22805 (LRM93_22805) | 38898..39890 | - | 993 | WP_000833321 | conjugal transfer pilus assembly protein TraU | traU |
LRM93_RS22810 (LRM93_22810) | 39887..40519 | - | 633 | WP_000871997 | type-F conjugative transfer system protein TraW | traW |
LRM93_RS22815 (LRM93_22815) | 40516..40902 | - | 387 | WP_000105164 | type-F conjugative transfer system protein TrbI | - |
LRM93_RS22820 (LRM93_22820) | 40877..43525 | - | 2649 | WP_000793311 | type IV secretion system protein TraC | virb4 |
LRM93_RS22825 (LRM93_22825) | 43541..43924 | - | 384 | WP_000870324 | hypothetical protein | - |
LRM93_RS22830 (LRM93_22830) | 43921..44388 | - | 468 | WP_000549560 | hypothetical protein | - |
LRM93_RS22835 (LRM93_22835) | 44381..44602 | - | 222 | WP_001278681 | conjugal transfer protein TraR | - |
LRM93_RS22840 (LRM93_22840) | 44737..45252 | - | 516 | WP_001079997 | type IV conjugative transfer system lipoprotein TraV | traV |
LRM93_RS22845 (LRM93_22845) | 45249..45473 | - | 225 | WP_077906918 | DUF2689 domain-containing protein | - |
LRM93_RS22850 (LRM93_22850) | 45451..46047 | - | 597 | WP_000527895 | conjugal transfer pilus-stabilizing protein TraP | - |
LRM93_RS22855 (LRM93_22855) | 46037..47458 | - | 1422 | WP_000146702 | F-type conjugal transfer pilus assembly protein TraB | traB |
LRM93_RS22860 (LRM93_22860) | 47458..48198 | - | 741 | WP_001230721 | type-F conjugative transfer system secretin TraK | traK |
LRM93_RS22865 (LRM93_22865) | 48185..48751 | - | 567 | WP_000399761 | type IV conjugative transfer system protein TraE | traE |
LRM93_RS22870 (LRM93_22870) | 48773..49084 | - | 312 | WP_000012129 | type IV conjugative transfer system protein TraL | traL |
LRM93_RS22875 (LRM93_22875) | 49099..49461 | - | 363 | WP_001274198 | type IV conjugative transfer system pilin TraA | - |
LRM93_RS22880 (LRM93_22880) | 49503..49730 | - | 228 | WP_001676655 | conjugal transfer relaxosome protein TraY | - |
LRM93_RS22885 (LRM93_22885) | 49824..50510 | - | 687 | WP_000336296 | PAS domain-containing protein | - |
LRM93_RS22890 (LRM93_22890) | 50703..51083 | - | 381 | WP_001151546 | conjugal transfer relaxosome DNA-binding protein TraM | - |
LRM93_RS22895 (LRM93_22895) | 51500..51970 | + | 471 | WP_015056628 | transglycosylase SLT domain-containing protein | virB1 |
LRM93_RS22900 (LRM93_22900) | 52493..53099 | - | 607 | Protein_61 | DUF932 domain-containing protein | - |
LRM93_RS22905 (LRM93_22905) | 53202..53360 | - | 159 | WP_001704449 | hypothetical protein | - |
LRM93_RS23245 | 53446..53574 | - | 129 | WP_000372430 | hypothetical protein | - |
LRM93_RS22910 (LRM93_22910) | 53609..54265 | - | 657 | Protein_64 | plasmid SOS inhibition protein A | - |
LRM93_RS22915 (LRM93_22915) | 54268..54369 | - | 102 | Protein_65 | conjugation system SOS inhibitor PsiB family protein | - |
LRM93_RS22920 (LRM93_22920) | 54409..56130 | - | 1722 | Protein_66 | ParB/RepB/Spo0J family partition protein | - |
LRM93_RS22925 (LRM93_22925) | 56431..56853 | + | 423 | WP_000925628 | translesion error-prone DNA polymerase V autoproteolytic subunit | - |
Host bacterium
ID | 9396 | GenBank | NZ_CP088143 |
Plasmid name | pB-4548_1 | Incompatibility group | IncFIB |
Plasmid size | 92906 bp | Coordinate of oriT [Strand] | 51168..51429 [-] |
Host baterium | Salmonella enterica subsp. enterica serovar Gallinarum strain SCPM-O-B-4548 |
Cargo genes
Drug resistance gene | - |
Virulence gene | - |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | - |