Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 108858 |
| Name | oriT_pAA04  |
| Organism | Aminobacter aminovorans strain KCTC 2477 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP015009 (111681..111711 [-], 31 nt) |
| oriT length | 31 nt |
| IRs (inverted repeats) | 16..21, 26..31 (CGTCGC..GCGACG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 31 nt
>oriT_pAA04
AAGGGCGCAGTAATACGTCGCCGGCGCGACG
AAGGGCGCAGTAATACGTCGCCGGCGCGACG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 5938 | GenBank | WP_067970316 |
| Name | traA_AA2016_RS33055_pAA04 |
UniProt ID | _ |
| Length | 1100 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1100 a.a. Molecular weight: 123218.45 Da Isoelectric Point: 9.8907
>WP_067970316.1 Ti-type conjugative transfer relaxase TraA [Aminobacter aminovorans]
MAITHFTPQLIGRSSGRSAVLATAYRHCAKMTHDSEGRTVDFSNKGNLGHEEFLLPPDAPQWARALIADR
SVAGAAEAFWNRVEAFEKREDAQFAKEFIIALPVELSKEQNIALMRQFVLEHMLSRGQIADWVYHDEPGN
PHVHLMTTLRPLTENGFGPKKIAVKGDDGAVLRTNAGKIQYRLWAGEKQEFLEQRNGWLDLQNQHLALAG
LEIRVDGRSYAERGIELEPTAHIGAGAKAIQRKAEREEWTPSLERIEIAETRKSANAARILSRPEIVLDL
LTSEMSVFDERDIAKVLHRYVDDAGTFQELTARILQSPEMLRLERPTVDFETGRKIPSRYTTREMIRLEA
GMANRTIWLSAKATHGVRDRVLESVFDRHERLSDEQRTAIEQVTKPGRIAAVVGRAGAGKTTMMKAAREA
WELAGYRVIGGTLAGKAAEGLQKEAGIESRTLASWELRWKEGREPLTDKTVMVIDEAGMVASKQMAHFVE
TAFRFGAKLVLIGDPEQLQPIEAGAAFRAIVERIGYAELERIYRQREDWMRTASLDLARGNVAKAVGAYS
GHGRIIGEKLKAQAITSLIAAWDHEYDPAKSTLILAHLRRDVRELNRLAREKMVERGAVGEGHAFRTEDG
LRTFDAGDQIVFLKNDSALGVKNGMLARVVEAAPGRIVAEVGEGSDRRHVAVEQRLYCNVDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHREDVTLYFGKRSFEKAGGLEHILSRRNTKETTLDYERGTLY
RQALSFAENRGMHIVKVARTLVRDQLDWTLRQKQRLAGMASRLRSIGARLGLLDTRQSQSQKEKKPMVAG
IKAFAKSVPDIVEEKLRADPAVKKQWEEVSTRFRYVFADPETAFKAMNFDAVLADRDAAKQALDKLVSEP
ASIGPLKGRTGVLAGKTQREERRVAELNVPALKRDIERYLDLRQTAGEKIAAEEQTVRRRVSIDIPALSS
DAYRVLERVRDAIDRNDLPAALGYAVSNREIKTEIDGFTKAVAERFGQRSLLVNEARSPSGKLFDKAAEG
MAPAEKQKLDEAWPVMRAAQQLAAHERTSEAMKQSETLRLSQRHNSVLKQ
MAITHFTPQLIGRSSGRSAVLATAYRHCAKMTHDSEGRTVDFSNKGNLGHEEFLLPPDAPQWARALIADR
SVAGAAEAFWNRVEAFEKREDAQFAKEFIIALPVELSKEQNIALMRQFVLEHMLSRGQIADWVYHDEPGN
PHVHLMTTLRPLTENGFGPKKIAVKGDDGAVLRTNAGKIQYRLWAGEKQEFLEQRNGWLDLQNQHLALAG
LEIRVDGRSYAERGIELEPTAHIGAGAKAIQRKAEREEWTPSLERIEIAETRKSANAARILSRPEIVLDL
LTSEMSVFDERDIAKVLHRYVDDAGTFQELTARILQSPEMLRLERPTVDFETGRKIPSRYTTREMIRLEA
GMANRTIWLSAKATHGVRDRVLESVFDRHERLSDEQRTAIEQVTKPGRIAAVVGRAGAGKTTMMKAAREA
WELAGYRVIGGTLAGKAAEGLQKEAGIESRTLASWELRWKEGREPLTDKTVMVIDEAGMVASKQMAHFVE
TAFRFGAKLVLIGDPEQLQPIEAGAAFRAIVERIGYAELERIYRQREDWMRTASLDLARGNVAKAVGAYS
GHGRIIGEKLKAQAITSLIAAWDHEYDPAKSTLILAHLRRDVRELNRLAREKMVERGAVGEGHAFRTEDG
LRTFDAGDQIVFLKNDSALGVKNGMLARVVEAAPGRIVAEVGEGSDRRHVAVEQRLYCNVDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHREDVTLYFGKRSFEKAGGLEHILSRRNTKETTLDYERGTLY
RQALSFAENRGMHIVKVARTLVRDQLDWTLRQKQRLAGMASRLRSIGARLGLLDTRQSQSQKEKKPMVAG
IKAFAKSVPDIVEEKLRADPAVKKQWEEVSTRFRYVFADPETAFKAMNFDAVLADRDAAKQALDKLVSEP
ASIGPLKGRTGVLAGKTQREERRVAELNVPALKRDIERYLDLRQTAGEKIAAEEQTVRRRVSIDIPALSS
DAYRVLERVRDAIDRNDLPAALGYAVSNREIKTEIDGFTKAVAERFGQRSLLVNEARSPSGKLFDKAAEG
MAPAEKQKLDEAWPVMRAAQQLAAHERTSEAMKQSETLRLSQRHNSVLKQ
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 6395 | GenBank | WP_067970078 |
| Name | t4cp2_AA2016_RS32575_pAA04 |
UniProt ID | _ |
| Length | 818 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 818 a.a. Molecular weight: 91147.56 Da Isoelectric Point: 5.9480
>WP_067970078.1 conjugal transfer protein TrbE [Aminobacter aminovorans]
MTALRAFRHAGPSFADLVPYAALVANGVILLKDGSLMAGWYFAGPDSESSTDAERNEVARQINAVLSRLG
SGWMIQVEAVRVATSAYPSREDCHFPDPVTRAIDEERRAHFEKGRGHFESQHALILTWRPPEKTRSGLAR
YVYSDSESRGARYADTVLESFQTSIREVEQYLGNTLSIRRMVTQEVEERGGFRSARYDELFQFIRFCITG
ENHPVRLTEIPLYLDWLATAEFHHGVSPMVENRYLAVVAIDGFPAESWPGILNALDLMPLSYRWSSRFMF
LDDQEARQKLERTRKKWQQKVRPFFDQLFQTQSRSVDQDAMLMVAETEDAIAQASSQLVSYGYYTPVVVL
FDEDEPRLREKAEAARRLIQAEGFGARIETINATEAFLGSLPGNWYANIREPLISTRNLADLIPMNSVWS
GAPVAPCPFYPPGAPPLMQVASGSTPFRLNLHVDDVGHTLIFGPTGSGKSTLLALIAAQFRRYAGAQIFA
FDKGRSLLPLTLAAGGDHYEVGEGEGSHGPALAFCPLADLATDGDRAWASEWIETLVALQGVAITPDHRN
AISRQVGLMAEARGRSISDFVSGVQMREIKEALHTYTVDGPMGQLLDAEQDGLSLGLFQTFEIEQLMNLG
ERSLVPVLTYLFRRIEKRLTGAPSLIILDEAWLMLGHPVFHDKIREWLKVLRKANCAVVLATQSISDAER
SGIIDVLKESCPTKICLPNGAAREPGTREFYERIGFNERQIEIVATSLPKREYYVVSPIGRRLFDMALGP
VALAFAGASGRDDLKRISALRTEHGSDWPVHWLQQRGSSDAQALLHVS
MTALRAFRHAGPSFADLVPYAALVANGVILLKDGSLMAGWYFAGPDSESSTDAERNEVARQINAVLSRLG
SGWMIQVEAVRVATSAYPSREDCHFPDPVTRAIDEERRAHFEKGRGHFESQHALILTWRPPEKTRSGLAR
YVYSDSESRGARYADTVLESFQTSIREVEQYLGNTLSIRRMVTQEVEERGGFRSARYDELFQFIRFCITG
ENHPVRLTEIPLYLDWLATAEFHHGVSPMVENRYLAVVAIDGFPAESWPGILNALDLMPLSYRWSSRFMF
LDDQEARQKLERTRKKWQQKVRPFFDQLFQTQSRSVDQDAMLMVAETEDAIAQASSQLVSYGYYTPVVVL
FDEDEPRLREKAEAARRLIQAEGFGARIETINATEAFLGSLPGNWYANIREPLISTRNLADLIPMNSVWS
GAPVAPCPFYPPGAPPLMQVASGSTPFRLNLHVDDVGHTLIFGPTGSGKSTLLALIAAQFRRYAGAQIFA
FDKGRSLLPLTLAAGGDHYEVGEGEGSHGPALAFCPLADLATDGDRAWASEWIETLVALQGVAITPDHRN
AISRQVGLMAEARGRSISDFVSGVQMREIKEALHTYTVDGPMGQLLDAEQDGLSLGLFQTFEIEQLMNLG
ERSLVPVLTYLFRRIEKRLTGAPSLIILDEAWLMLGHPVFHDKIREWLKVLRKANCAVVLATQSISDAER
SGIIDVLKESCPTKICLPNGAAREPGTREFYERIGFNERQIEIVATSLPKREYYVVSPIGRRLFDMALGP
VALAFAGASGRDDLKRISALRTEHGSDWPVHWLQQRGSSDAQALLHVS
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 6396 | GenBank | WP_067970325 |
| Name | traG_AA2016_RS33070_pAA04 |
UniProt ID | _ |
| Length | 643 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 643 a.a. Molecular weight: 69892.85 Da Isoelectric Point: 9.4954
>WP_067970325.1 Ti-type conjugative transfer system protein TraG [Aminobacter aminovorans]
MTPHRILLFVTPVVQMIGILFLTTGIEHWTAQFGSSEQARLMLGRIGIAMPYGLAAMSGVVFLFAAKGAA
AIRSAGLGVLIGAAGVVAVAVFRETTRLAGLADSVPAGQSILSYVDPATGMGSVIVIFASLFAARVALRG
NAAFASGAPRRIRGKRAIHGEADWMSLGDARRLFPEKGGIVVGEAYRVDKDSSADRGFRAEDPETWGVGG
KSPLLCFDASFGSTHGLVFAGSGGFKTTSVTVPTALKWGGGLVALDPSNEVAPMVIEHRRKAGRDVFVLD
PKHPTTGFNVLDWIGRFGGSKEEDVVAVANWVVTDTGRQASIRDDFFRASALQLITALIADVCLSGHTEK
ERQHLRQMRANLSEPEPKLRERLQLIYDNSESEFVKENVAPFIAMTPETFSGVYANAVKETHWLSYPNYA
ALVSGSSFTTDSVADGKTDIFVNLDLKTLEAHPGLARTVIGALLNAIYNRNGEVPGRTLFLLDEVARLGY
LRVLETARDAGRKYGISLVLLFQSIGQMRETYGGRDATSKWFESASWVSFAAINDPDTADYISRRCGNTT
VEVDQLSRSSQMSGSSRTRSKQLAARPLILPEEVLRMRGDEQIVFTAGNAPLRCGRAIWFRRDDMKAVMA
ENRFHRRRQREAP
MTPHRILLFVTPVVQMIGILFLTTGIEHWTAQFGSSEQARLMLGRIGIAMPYGLAAMSGVVFLFAAKGAA
AIRSAGLGVLIGAAGVVAVAVFRETTRLAGLADSVPAGQSILSYVDPATGMGSVIVIFASLFAARVALRG
NAAFASGAPRRIRGKRAIHGEADWMSLGDARRLFPEKGGIVVGEAYRVDKDSSADRGFRAEDPETWGVGG
KSPLLCFDASFGSTHGLVFAGSGGFKTTSVTVPTALKWGGGLVALDPSNEVAPMVIEHRRKAGRDVFVLD
PKHPTTGFNVLDWIGRFGGSKEEDVVAVANWVVTDTGRQASIRDDFFRASALQLITALIADVCLSGHTEK
ERQHLRQMRANLSEPEPKLRERLQLIYDNSESEFVKENVAPFIAMTPETFSGVYANAVKETHWLSYPNYA
ALVSGSSFTTDSVADGKTDIFVNLDLKTLEAHPGLARTVIGALLNAIYNRNGEVPGRTLFLLDEVARLGY
LRVLETARDAGRKYGISLVLLFQSIGQMRETYGGRDATSKWFESASWVSFAAINDPDTADYISRRCGNTT
VEVDQLSRSSQMSGSSRTRSKQLAARPLILPEEVLRMRGDEQIVFTAGNAPLRCGRAIWFRRDDMKAVMA
ENRFHRRRQREAP
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 2215..11801
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AA2016_RS32555 (AA2016_6541) | 1577..2200 | + | 624 | WP_067970070 | acyl-homoserine-lactone synthase TraI | - |
| AA2016_RS32560 (AA2016_6542) | 2215..3201 | + | 987 | WP_067970381 | P-type conjugative transfer ATPase TrbB | virB11 |
| AA2016_RS32565 (AA2016_6543) | 3227..3580 | + | 354 | WP_083948947 | TrbC/VirB2 family protein | virB2 |
| AA2016_RS32570 (AA2016_6544) | 3573..3872 | + | 300 | WP_067970075 | conjugal transfer protein TrbD | virB3 |
| AA2016_RS32575 (AA2016_6545) | 3883..6339 | + | 2457 | WP_067970078 | conjugal transfer protein TrbE | virb4 |
| AA2016_RS32580 (AA2016_6546) | 6329..7105 | + | 777 | WP_246790414 | P-type conjugative transfer protein TrbJ | virB5 |
| AA2016_RS32585 (AA2016_6547) | 7102..7320 | + | 219 | WP_249926047 | entry exclusion protein TrbK | - |
| AA2016_RS32590 (AA2016_6548) | 7314..8501 | + | 1188 | WP_067970083 | P-type conjugative transfer protein TrbL | virB6 |
| AA2016_RS32595 (AA2016_6549) | 8520..9182 | + | 663 | WP_067970086 | conjugal transfer protein TrbF | virB8 |
| AA2016_RS32600 (AA2016_6550) | 9196..10035 | + | 840 | WP_083948948 | P-type conjugative transfer protein TrbG | virB9 |
| AA2016_RS32605 (AA2016_6551) | 10039..10485 | + | 447 | WP_067970088 | conjugal transfer protein TrbH | - |
| AA2016_RS32610 (AA2016_6552) | 10497..11801 | + | 1305 | WP_067970091 | IncP-type conjugal transfer protein TrbI | virB10 |
| AA2016_RS34005 | 12622..12918 | + | 297 | Protein_13 | helix-turn-helix domain-containing protein | - |
| AA2016_RS35530 | 12903..13684 | - | 782 | Protein_14 | transposase | - |
| AA2016_RS32630 (AA2016_6556) | 13748..14065 | + | 318 | Protein_15 | ISNCY family transposase | - |
| AA2016_RS32635 | 14144..15301 | - | 1158 | Protein_16 | IS110 family transposase | - |
| AA2016_RS34010 | 15452..15780 | + | 329 | Protein_17 | integrase core domain-containing protein | - |
Host bacterium
| ID | 9293 | GenBank | NZ_CP015009 |
| Plasmid name | pAA04 | Incompatibility group | - |
| Plasmid size | 138061 bp | Coordinate of oriT [Strand] | 111681..111711 [-] |
| Host baterium | Aminobacter aminovorans strain KCTC 2477 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | bphC |
| Symbiosis gene | - |
| Anti-CRISPR | - |