Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 108857 |
| Name | oriT_pAA03  |
| Organism | Aminobacter aminovorans strain KCTC 2477 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP015008 (109863..109899 [+], 37 nt) |
| oriT length | 37 nt |
| IRs (inverted repeats) | 22..27, 32..37 (CGTCGC..GCGACG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 37 nt
>oriT_pAA03
GGATCCAAGGGCGCAGTAATACGTCGCTGACGCGACG
GGATCCAAGGGCGCAGTAATACGTCGCTGACGCGACG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 5937 | GenBank | WP_067970056 |
| Name | traA_AA2016_RS32390_pAA03 |
UniProt ID | _ |
| Length | 1100 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1100 a.a. Molecular weight: 123647.41 Da Isoelectric Point: 10.1861
>WP_067970056.1 Ti-type conjugative transfer relaxase TraA [Aminobacter aminovorans]
MAITHFTPQIIGRGNGRSAVLSAAYRHCAKMTYEMEARTVDYSNKRNLAHEEFLLPADAPDWVRKLIADR
SVAGAVEAFWNAVEAFEKREDAQLAKEFIVALPVELTKEQNIALMRQFVFEQVLARGQVADWVYHDEPGN
PHVHLMTTLRPLTETGFGAKKVAMLGDDGQPVRTKSGKIQYRLWAGEKQGFLEQRNRWLDLQNQHLALGG
LEIRVDGRSYAERGIDLVPTVHIGVGAKAIQRKGERQGWTPSLDRIEQFEAQRQENARRILRRPEIVLDL
LSSEKSVFGERDIAKVLHRYVDDARTFQHLMTRILQSPDLLRIERESVDFATGERVRARYTTRELIRVEA
EMAKRAIWLSERTSHSVRQKVLEATFARHERLSQEQRVAIEHVAGATRIAAIVGRAGAGKTTMMKAAREA
WESAGYRVVGGALAGKAAEGLEKEAGIASRTLASWELRWKDGRDAIDAKTVFVLDEAGMVASKQMALFVE
AALRAGAKLVLVGDPEQLQPIEAGAAFRAIVERIGYAELETIYRQREDWMRKASLDLARGRVDQAISAYR
GRGRVMGSELKVQAVENLISDWNGDYDATKSTLILAHLRRDVRMLNVMAREKLVERGIVGDGHAFRTEDG
TRRFDAGDQIVFLKNDGALGVKNGMIGKVVEAAPNRIVAEIGEGDQRHRVSVDQRFYRNLDHGYATTIHK
SQGSTVDRVKVLASLSLDRHLTYVALTRHRKDMALYYGRRSFGMNGGLVKILSRRNAKETTLDYERGTLY
RQALRFAENRGLHIARVARTLVRDRVDWTLRQKQRLADLGLRLRGLGEHLGLLDTRQTQTLKEAAPMVKG
VTQFPTSIQDTVEEKLLADLAVKKQWEEVSTRFRVVFADPETAFLAMNFDAALTDPSTARITLERLVNEP
ATIGPLKGRVGLLAGKVDREDRRIAEVNAPALKRDIECYLRLRLTAVQKIEADEQTLRRRVSIDIPALSP
AAIQVLERVRDAIDRNDLPGALGYALADRMAKVEIDGFNKAITERFGERTLLTNAAREPSGKLFERAADG
LSPGERQKLAEAWPAMRAAQQIAAHERTVETLKQTENLRLSQRQTPVLKQ
MAITHFTPQIIGRGNGRSAVLSAAYRHCAKMTYEMEARTVDYSNKRNLAHEEFLLPADAPDWVRKLIADR
SVAGAVEAFWNAVEAFEKREDAQLAKEFIVALPVELTKEQNIALMRQFVFEQVLARGQVADWVYHDEPGN
PHVHLMTTLRPLTETGFGAKKVAMLGDDGQPVRTKSGKIQYRLWAGEKQGFLEQRNRWLDLQNQHLALGG
LEIRVDGRSYAERGIDLVPTVHIGVGAKAIQRKGERQGWTPSLDRIEQFEAQRQENARRILRRPEIVLDL
LSSEKSVFGERDIAKVLHRYVDDARTFQHLMTRILQSPDLLRIERESVDFATGERVRARYTTRELIRVEA
EMAKRAIWLSERTSHSVRQKVLEATFARHERLSQEQRVAIEHVAGATRIAAIVGRAGAGKTTMMKAAREA
WESAGYRVVGGALAGKAAEGLEKEAGIASRTLASWELRWKDGRDAIDAKTVFVLDEAGMVASKQMALFVE
AALRAGAKLVLVGDPEQLQPIEAGAAFRAIVERIGYAELETIYRQREDWMRKASLDLARGRVDQAISAYR
GRGRVMGSELKVQAVENLISDWNGDYDATKSTLILAHLRRDVRMLNVMAREKLVERGIVGDGHAFRTEDG
TRRFDAGDQIVFLKNDGALGVKNGMIGKVVEAAPNRIVAEIGEGDQRHRVSVDQRFYRNLDHGYATTIHK
SQGSTVDRVKVLASLSLDRHLTYVALTRHRKDMALYYGRRSFGMNGGLVKILSRRNAKETTLDYERGTLY
RQALRFAENRGLHIARVARTLVRDRVDWTLRQKQRLADLGLRLRGLGEHLGLLDTRQTQTLKEAAPMVKG
VTQFPTSIQDTVEEKLLADLAVKKQWEEVSTRFRVVFADPETAFLAMNFDAALTDPSTARITLERLVNEP
ATIGPLKGRVGLLAGKVDREDRRIAEVNAPALKRDIECYLRLRLTAVQKIEADEQTLRRRVSIDIPALSP
AAIQVLERVRDAIDRNDLPGALGYALADRMAKVEIDGFNKAITERFGERTLLTNAAREPSGKLFERAADG
LSPGERQKLAEAWPAMRAAQQIAAHERTVETLKQTENLRLSQRQTPVLKQ
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 6393 | GenBank | WP_067969933 |
| Name | traG_AA2016_RS32375_pAA03 |
UniProt ID | _ |
| Length | 641 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 641 a.a. Molecular weight: 68744.62 Da Isoelectric Point: 8.9336
>WP_067969933.1 Ti-type conjugative transfer system protein TraG [Aminobacter aminovorans]
MTANRLLLAGSAAALMLGACIWISGVETWLAGFGTSDAARLMLGRIGIALPYAAAAVIGVIFLFASAGAS
AIRAAGWGVAGGAAGVIAIAVAREAVRLSVLADRVPAGKTILSYADPATMAGATIAMICGGFALRVAIRG
NAAFAVAVPRRIRGKRAVHGEADWMSMPAAAQLFPDSAGIVIGERYRLGRDSVAAQSFRADARETWGAGG
RSPLLCFDGSFGSSHGVVFAGSGGFKTTSVTVPTALKWGGSLVVLDPSSEVAPMVIDHRKRAGRNVFLLD
PGNAAIGFNALDWIGRFGSTKEEDIVAVATWIITDNPRQASARDDFFRASALQLLTVLIADVCLSGHTEE
KDQTLRRVRSNLSEPEPKLRERLTAIHDQSESAFVKENVAPFIAMTPETFSGVYANAVKETHWLSYSNYA
ALVSGSSFTTDELAAGETDIFIALDLKVLETHPGLARVIIGALMNAIYNRNGEVKGRTLFLLDEVARLGF
LRVLETARDAGRKYGITMTLLYQSIGQMREAYGGRDATSKWFESASWVSFAAINDPETADYISKRCGDTT
VEVDQTSRSSQMSGSSHTRSKQLTRRPLILPHEVMQMRADEQIVFTAGNPPLRCGRAIWFRRADMKACVG
ENRFHRKETEA
MTANRLLLAGSAAALMLGACIWISGVETWLAGFGTSDAARLMLGRIGIALPYAAAAVIGVIFLFASAGAS
AIRAAGWGVAGGAAGVIAIAVAREAVRLSVLADRVPAGKTILSYADPATMAGATIAMICGGFALRVAIRG
NAAFAVAVPRRIRGKRAVHGEADWMSMPAAAQLFPDSAGIVIGERYRLGRDSVAAQSFRADARETWGAGG
RSPLLCFDGSFGSSHGVVFAGSGGFKTTSVTVPTALKWGGSLVVLDPSSEVAPMVIDHRKRAGRNVFLLD
PGNAAIGFNALDWIGRFGSTKEEDIVAVATWIITDNPRQASARDDFFRASALQLLTVLIADVCLSGHTEE
KDQTLRRVRSNLSEPEPKLRERLTAIHDQSESAFVKENVAPFIAMTPETFSGVYANAVKETHWLSYSNYA
ALVSGSSFTTDELAAGETDIFIALDLKVLETHPGLARVIIGALMNAIYNRNGEVKGRTLFLLDEVARLGF
LRVLETARDAGRKYGITMTLLYQSIGQMREAYGGRDATSKWFESASWVSFAAINDPETADYISKRCGDTT
VEVDQTSRSSQMSGSSHTRSKQLTRRPLILPHEVMQMRADEQIVFTAGNPPLRCGRAIWFRRADMKACVG
ENRFHRKETEA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 6394 | GenBank | WP_067970011 |
| Name | t4cp2_AA2016_RS32530_pAA03 |
UniProt ID | _ |
| Length | 818 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 818 a.a. Molecular weight: 91474.94 Da Isoelectric Point: 5.7517
>WP_067970011.1 conjugal transfer protein TrbE [Aminobacter aminovorans]
MAALRSFRHSGPSFSDLVPYAALVANGVILLKDGSLMAGWYFAGPDSESSTDAERNEVSRQINTVLSRLG
SGWMIQVEAVRVATSDYPAHEQCHFPDPVTLAIDGERRAHFQKERGHYESRHALILTWRPPEKTRSGLAR
YIYSDSESRAARYADTVLDSFQTSIREVEQYLGNVLSIRRMVTQSVEERGGFQTARYDELFQFIRFCITG
ENHPIRLPEIPMYLDWLATAEFHHGLSPMVENHYLAVVAIDGFPAESWPGILNSLDLMPLTYRWSSRFIF
LDDQEARQKLERTRKKWQQKVRPFFDQLFQTQSRSVDQDAMMMVAETEDAIAQASSQLVAYGYYTPVIVL
FDDSEARLREKAEAVRRLIQAEGFGARIETVNATEAFLGSLPGNWFANIREPLINTRNLADLIPLNSVWS
GAPAAPCPFYPPASPPLMLVASGSTPFRLNLHVDDVGHTLIFGPTGSGKSTLLSLIAAQFRRYAFAQIFA
FDKGRSMLPLTLAAGGDHYEIGETEGEGGARLAFCPLSDLATDGDRAWASEWIETLVVLQGVPITPDHRN
AISRQVALMADSSGRSLSDFVSGVQLREIKEALHHYTVDGPLGQLLDAERDGLSLGSFQTFEIEQLMNMG
ERSLVPVLLYLFRRIEKRLTGAPSLIILDEAWLMLGHATFRDKIREWLKVLRKANCAVVLATQSISDAER
SGIIDVLKESCPTKICLPNGAARERGTREFYERIGFNERQIEIVATALPKREYYVVSPDGRRLFDMALGP
VALAFAGASGKEDLKRIQALSAEHGDDWPIHWLQQRGIGDAETILERG
MAALRSFRHSGPSFSDLVPYAALVANGVILLKDGSLMAGWYFAGPDSESSTDAERNEVSRQINTVLSRLG
SGWMIQVEAVRVATSDYPAHEQCHFPDPVTLAIDGERRAHFQKERGHYESRHALILTWRPPEKTRSGLAR
YIYSDSESRAARYADTVLDSFQTSIREVEQYLGNVLSIRRMVTQSVEERGGFQTARYDELFQFIRFCITG
ENHPIRLPEIPMYLDWLATAEFHHGLSPMVENHYLAVVAIDGFPAESWPGILNSLDLMPLTYRWSSRFIF
LDDQEARQKLERTRKKWQQKVRPFFDQLFQTQSRSVDQDAMMMVAETEDAIAQASSQLVAYGYYTPVIVL
FDDSEARLREKAEAVRRLIQAEGFGARIETVNATEAFLGSLPGNWFANIREPLINTRNLADLIPLNSVWS
GAPAAPCPFYPPASPPLMLVASGSTPFRLNLHVDDVGHTLIFGPTGSGKSTLLSLIAAQFRRYAFAQIFA
FDKGRSMLPLTLAAGGDHYEIGETEGEGGARLAFCPLSDLATDGDRAWASEWIETLVVLQGVPITPDHRN
AISRQVALMADSSGRSLSDFVSGVQLREIKEALHHYTVDGPLGQLLDAERDGLSLGSFQTFEIEQLMNMG
ERSLVPVLLYLFRRIEKRLTGAPSLIILDEAWLMLGHATFRDKIREWLKVLRKANCAVVLATQSISDAER
SGIIDVLKESCPTKICLPNGAARERGTREFYERIGFNERQIEIVATALPKREYYVVSPDGRRLFDMALGP
VALAFAGASGKEDLKRIQALSAEHGDDWPIHWLQQRGIGDAETILERG
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 133043..142600
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AA2016_RS32480 (AA2016_6526) | 129004..129948 | - | 945 | WP_067969989 | ABC transporter permease | - |
| AA2016_RS32485 (AA2016_6527) | 130005..131534 | - | 1530 | WP_083948944 | sugar ABC transporter ATP-binding protein | - |
| AA2016_RS32490 (AA2016_6528) | 131655..132605 | + | 951 | WP_246790424 | LysR substrate-binding domain-containing protein | - |
| AA2016_RS32495 (AA2016_6529) | 133043..134347 | - | 1305 | WP_067969994 | IncP-type conjugal transfer protein TrbI | virB10 |
| AA2016_RS32500 (AA2016_6530) | 134359..134811 | - | 453 | WP_067969997 | conjugal transfer protein TrbH | - |
| AA2016_RS32505 (AA2016_6531) | 134815..135642 | - | 828 | WP_067970067 | P-type conjugative transfer protein TrbG | virB9 |
| AA2016_RS32510 (AA2016_6532) | 135660..136322 | - | 663 | WP_067970000 | conjugal transfer protein TrbF | virB8 |
| AA2016_RS32515 (AA2016_6533) | 136341..137528 | - | 1188 | WP_067970002 | P-type conjugative transfer protein TrbL | virB6 |
| AA2016_RS32520 (AA2016_6534) | 137522..137731 | - | 210 | WP_067970005 | entry exclusion protein TrbK | - |
| AA2016_RS32525 (AA2016_6535) | 137728..138468 | - | 741 | WP_246790426 | P-type conjugative transfer protein TrbJ | virB5 |
| AA2016_RS32530 (AA2016_6536) | 138500..140956 | - | 2457 | WP_067970011 | conjugal transfer protein TrbE | virb4 |
| AA2016_RS32535 (AA2016_6537) | 140967..141278 | - | 312 | WP_067970014 | conjugal transfer protein TrbD | virB3 |
| AA2016_RS32540 (AA2016_6538) | 141271..141642 | - | 372 | WP_067970017 | TrbC/VirB2 family protein | virB2 |
| AA2016_RS32545 (AA2016_6539) | 141632..142600 | - | 969 | WP_067970023 | P-type conjugative transfer ATPase TrbB | virB11 |
Host bacterium
| ID | 9292 | GenBank | NZ_CP015008 |
| Plasmid name | pAA03 | Incompatibility group | - |
| Plasmid size | 142965 bp | Coordinate of oriT [Strand] | 109863..109899 [+] |
| Host baterium | Aminobacter aminovorans strain KCTC 2477 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA9 |