Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 108856 |
| Name | oriT_pAA02  |
| Organism | Aminobacter aminovorans strain KCTC 2477 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP015007 (155984..156023 [+], 40 nt) |
| oriT length | 40 nt |
| IRs (inverted repeats) | 19..24, 29..34 (CGTCGC..GCGACG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 40 nt
>oriT_pAA02
TCCAAGGGCGCAATTATACGTCGCTGACGCGACGCCTTGC
TCCAAGGGCGCAATTATACGTCGCTGACGCGACGCCTTGC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 5936 | GenBank | WP_067968890 |
| Name | traA_AA2016_RS30540_pAA02 |
UniProt ID | _ |
| Length | 1100 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1100 a.a. Molecular weight: 123388.33 Da Isoelectric Point: 10.1311
>WP_067968890.1 Ti-type conjugative transfer relaxase TraA [Aminobacter aminovorans]
MAVPHFSVSIVSRGDGRSAVLSAAYRHCAKMEYEREARSVDYTRKQGLLHEAFSVPPDAPQWLRSLIADR
SVAGASEAFWNKVEAFEKRSDAQLAKDITIALPLELSAEQNIALVRDFVERHILARGMVADWVYHDAPGN
PHVHLMMILRPLTEDGFGSKKVAVMGENGEVLRTKTGKIVYELWAGGIDDFTALRDAWFEIQNRHLALGG
IDLRVDGRSYAERGIDLVPTTHIGVGAKAIQRKSAREGHEMSLERIELLEARRKENASRILKRPEIVLDI
VSSEKSVFDERDIAKILHRYVDDAVTFQQLMVRILQSPEALRIERDGIDLATGARIRSRYTTRELIRIEA
KMAKQSLWLSQRTSHGVRQKVLDSVLARHAQLSQEQRVAIEHVVDEARIAAVVGRAGAGKTTMMKAAREA
WELAGYRVVGAALAGKAAEGLEKEAGIVGRTLASWELRWKQGRDMLDKSTVFVVDEAGMVASKQMALVVE
AATLAGAKLVLVGDPEQLQPIEAGAAFRAIVDRIGYAELETIYRQHEEWMRAASHNLARGRVAQALGVYS
EHGRVRGSELKAQAIANLIADWNSDYDPTKTTLILAHLRRDVRALNSLAREKLVERGIVGMGHDFRTEDG
LRRFDAGDQIVFLKNETSLGVKNGMIGRVVEAAPNRIVAAIGEGEHRRLVKVEQRFYPNIDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHREDMALYYGRRSFAKSGGLETILSRTNAKETTLDYERGTLY
RQALRFAGNRGLHISRVARTLVRDRLDWMLRQKMRLADLAQRFRVHGERLGLLQSPTMQIRNRAEPMVAG
VTLFARSLADVVEERLLADPALAKQWEEMSTRFRYVFADPEAAFRAMDFNALLTDQRVAQHTLAQLVSEP
ASLGALKGRTGLLASKADKEARRVAEVNVPALKRDIERYLQMRTVATERIEKEEQAHRHRLSIEIPALSP
TARSVLETIREAIDRNDLPAALAHIRDNREVRLEIDGFNRAIAERLGERTLLTHAAREASGSLFDKAAEG
LRPVEREKLRQAWPVMRAAQQIAAHERTTQTLTQVEQLRLAQRQGLAWKL
MAVPHFSVSIVSRGDGRSAVLSAAYRHCAKMEYEREARSVDYTRKQGLLHEAFSVPPDAPQWLRSLIADR
SVAGASEAFWNKVEAFEKRSDAQLAKDITIALPLELSAEQNIALVRDFVERHILARGMVADWVYHDAPGN
PHVHLMMILRPLTEDGFGSKKVAVMGENGEVLRTKTGKIVYELWAGGIDDFTALRDAWFEIQNRHLALGG
IDLRVDGRSYAERGIDLVPTTHIGVGAKAIQRKSAREGHEMSLERIELLEARRKENASRILKRPEIVLDI
VSSEKSVFDERDIAKILHRYVDDAVTFQQLMVRILQSPEALRIERDGIDLATGARIRSRYTTRELIRIEA
KMAKQSLWLSQRTSHGVRQKVLDSVLARHAQLSQEQRVAIEHVVDEARIAAVVGRAGAGKTTMMKAAREA
WELAGYRVVGAALAGKAAEGLEKEAGIVGRTLASWELRWKQGRDMLDKSTVFVVDEAGMVASKQMALVVE
AATLAGAKLVLVGDPEQLQPIEAGAAFRAIVDRIGYAELETIYRQHEEWMRAASHNLARGRVAQALGVYS
EHGRVRGSELKAQAIANLIADWNSDYDPTKTTLILAHLRRDVRALNSLAREKLVERGIVGMGHDFRTEDG
LRRFDAGDQIVFLKNETSLGVKNGMIGRVVEAAPNRIVAAIGEGEHRRLVKVEQRFYPNIDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHREDMALYYGRRSFAKSGGLETILSRTNAKETTLDYERGTLY
RQALRFAGNRGLHISRVARTLVRDRLDWMLRQKMRLADLAQRFRVHGERLGLLQSPTMQIRNRAEPMVAG
VTLFARSLADVVEERLLADPALAKQWEEMSTRFRYVFADPEAAFRAMDFNALLTDQRVAQHTLAQLVSEP
ASLGALKGRTGLLASKADKEARRVAEVNVPALKRDIERYLQMRTVATERIEKEEQAHRHRLSIEIPALSP
TARSVLETIREAIDRNDLPAALAHIRDNREVRLEIDGFNRAIAERLGERTLLTHAAREASGSLFDKAAEG
LRPVEREKLRQAWPVMRAAQQIAAHERTTQTLTQVEQLRLAQRQGLAWKL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 6391 | GenBank | WP_067968877 |
| Name | traG_AA2016_RS30520_pAA02 |
UniProt ID | _ |
| Length | 641 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 641 a.a. Molecular weight: 69156.28 Da Isoelectric Point: 8.9246
>WP_067968877.1 Ti-type conjugative transfer system protein TraG [Aminobacter aminovorans]
MTASKLLLAVTPATLMIASLFGSTGAGDWLAAFGTNDGARLILGHVVLALPCILSATVGIIFLFAAAGAA
NIKAAGWGVVTGSTVAILIAMVRETMRLSMLTGHLPVGQSALFYADPASILGVLVALFSGLFGFRVAVIG
NAAFARGAPRRIRGKRAVHGEAEWMSMQEAQRLFPDTGGIVVGERYRVDRDSVSAMAFRAYSKESWGAGG
RSPLLCFDGSFGSCHGMVFAGSGGFKTTSVTIPTALKWAGSLVVLDPSSEVAPMVIAHRRRAGRNVVVLD
PAEATIGFNALDWIGRFGSTKEEDIVAVATWVMTDNARQTSARDDFFRASALQLLTALIADVCLSGHTGP
KDQTLRQVRTNLSEPEPKLRERLTQIHQQSASAFVKENVAVFVNMTPETFSGVYANAVKETHWLSYPNYA
ALVSGEAFTTDDLANGGTDLFIALDLKVLGAHPGLARVIIGALMNAIYNRKGAVAAKTLFVLDEVARLGY
LSILETARDAGRKYGLTLIMIFQSIGQMREAYGGRDATSKWFESASWISFAAINDPDTADYISRRCGDTT
VEVDQVSRTSQTSGSSRSRSKQLYRRPLILPDEVMRMRSDEQIVFTAGNPPLRCGRAVWFRRDDMTGCVR
QNRFHLNEDEA
MTASKLLLAVTPATLMIASLFGSTGAGDWLAAFGTNDGARLILGHVVLALPCILSATVGIIFLFAAAGAA
NIKAAGWGVVTGSTVAILIAMVRETMRLSMLTGHLPVGQSALFYADPASILGVLVALFSGLFGFRVAVIG
NAAFARGAPRRIRGKRAVHGEAEWMSMQEAQRLFPDTGGIVVGERYRVDRDSVSAMAFRAYSKESWGAGG
RSPLLCFDGSFGSCHGMVFAGSGGFKTTSVTIPTALKWAGSLVVLDPSSEVAPMVIAHRRRAGRNVVVLD
PAEATIGFNALDWIGRFGSTKEEDIVAVATWVMTDNARQTSARDDFFRASALQLLTALIADVCLSGHTGP
KDQTLRQVRTNLSEPEPKLRERLTQIHQQSASAFVKENVAVFVNMTPETFSGVYANAVKETHWLSYPNYA
ALVSGEAFTTDDLANGGTDLFIALDLKVLGAHPGLARVIIGALMNAIYNRKGAVAAKTLFVLDEVARLGY
LSILETARDAGRKYGLTLIMIFQSIGQMREAYGGRDATSKWFESASWISFAAINDPDTADYISRRCGDTT
VEVDQVSRTSQTSGSSRSRSKQLYRRPLILPDEVMRMRSDEQIVFTAGNPPLRCGRAVWFRRDDMTGCVR
QNRFHLNEDEA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 6392 | GenBank | WP_067969586 |
| Name | t4cp2_AA2016_RS31895_pAA02 |
UniProt ID | _ |
| Length | 825 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 825 a.a. Molecular weight: 91973.80 Da Isoelectric Point: 6.1054
>WP_067969586.1 conjugal transfer protein TrbE [Aminobacter aminovorans]
MAALRPFRHSGPSFADLVPYAGLIANGVILLKDGSLMAGWYFAGPDSESSTDAERNEISRQINAILSRLG
SGWMVQVEAVRLPTVAYPATAECHFPDPVTRAIDAERRAHFEREKGHYESRHALILTYHPPTRHRSGLTR
YLYSDPESRAATYADKVLETFQTAIREVEQYLANVLSIRRMETRSAEERGGFLTAHYDELFQFIRFCITG
ENHPVRLPDIPMYLDWLVTAELQHGLTPIVESRFLGVVAIDGLPAESWPGILNSLDLMPLTYRWSSRFMF
LDDQEARQKLERTRKKWQQKVRPFFDQLFQTQSRSIDQDAMTMVAETEDAIAQASSQLVAYGYYTPVIVL
FDESSTRLRENCEAVRRLIQAEGFGARIETLNATEAFLGSLPGNWYANVREPLIHTRNLSDLIPLNSVWS
GSPIAPCPFYPPASPPLMQVASGSTPFRLNLHVEDVGHTLIFGPTGSGKSTLLALIVAQFRRYPGAQIFA
FDKGRSMLPLTLAAGGDHYEIGADSDVSTGEGASSVSALAFCPLSDLATDADRAFASEWIETLVALQGIS
ITPDHRNAISRQVALLTDARGRSLSDFVSGVQMREIKEALHHYTVDGPMGQLLDAESDGLSLGSLQTFEI
EELMNMGERNLVPVLTYLFRKIEKRLTGAPSLIVLDEAWLMLGHPTFRDKIREWLKVLRKANCAVVLATQ
SISDAERSGIIDVLKESCPTKICLPNGAAREAGTREFYQRIGFNERQIEIVATAVPKREYYVASPEGRRL
FDMSLGPLALSFLGATGKQDLQRILALKREHGADWPIYWLEMRGIAHAAAFFPST
MAALRPFRHSGPSFADLVPYAGLIANGVILLKDGSLMAGWYFAGPDSESSTDAERNEISRQINAILSRLG
SGWMVQVEAVRLPTVAYPATAECHFPDPVTRAIDAERRAHFEREKGHYESRHALILTYHPPTRHRSGLTR
YLYSDPESRAATYADKVLETFQTAIREVEQYLANVLSIRRMETRSAEERGGFLTAHYDELFQFIRFCITG
ENHPVRLPDIPMYLDWLVTAELQHGLTPIVESRFLGVVAIDGLPAESWPGILNSLDLMPLTYRWSSRFMF
LDDQEARQKLERTRKKWQQKVRPFFDQLFQTQSRSIDQDAMTMVAETEDAIAQASSQLVAYGYYTPVIVL
FDESSTRLRENCEAVRRLIQAEGFGARIETLNATEAFLGSLPGNWYANVREPLIHTRNLSDLIPLNSVWS
GSPIAPCPFYPPASPPLMQVASGSTPFRLNLHVEDVGHTLIFGPTGSGKSTLLALIVAQFRRYPGAQIFA
FDKGRSMLPLTLAAGGDHYEIGADSDVSTGEGASSVSALAFCPLSDLATDADRAFASEWIETLVALQGIS
ITPDHRNAISRQVALLTDARGRSLSDFVSGVQMREIKEALHHYTVDGPMGQLLDAESDGLSLGSLQTFEI
EELMNMGERNLVPVLTYLFRKIEKRLTGAPSLIVLDEAWLMLGHPTFRDKIREWLKVLRKANCAVVLATQ
SISDAERSGIIDVLKESCPTKICLPNGAAREAGTREFYQRIGFNERQIEIVATAVPKREYYVASPEGRRL
FDMSLGPLALSFLGATGKQDLQRILALKREHGADWPIYWLEMRGIAHAAAFFPST
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 439102..448692
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AA2016_RS31845 (AA2016_6401) | 434565..436223 | - | 1659 | WP_067969551 | ABC transporter substrate-binding protein | - |
| AA2016_RS34795 (AA2016_6402) | 436525..437436 | - | 912 | WP_169808413 | NAD(P)-dependent oxidoreductase | - |
| AA2016_RS31855 (AA2016_6403) | 437576..438922 | + | 1347 | WP_067969561 | MmgE/PrpD family protein | - |
| AA2016_RS31860 (AA2016_6404) | 439102..440406 | - | 1305 | WP_067969563 | IncP-type conjugal transfer protein TrbI | virB10 |
| AA2016_RS31865 (AA2016_6405) | 440419..440853 | - | 435 | WP_067969566 | conjugal transfer protein TrbH | - |
| AA2016_RS31870 (AA2016_6406) | 440855..441706 | - | 852 | WP_083948931 | P-type conjugative transfer protein TrbG | virB9 |
| AA2016_RS31875 (AA2016_6407) | 441724..442386 | - | 663 | WP_067969568 | conjugal transfer protein TrbF | virB8 |
| AA2016_RS31880 (AA2016_6408) | 442404..443594 | - | 1191 | WP_067969571 | P-type conjugative transfer protein TrbL | virB6 |
| AA2016_RS31885 | 443588..443788 | - | 201 | WP_067969573 | entry exclusion protein TrbK | - |
| AA2016_RS31890 (AA2016_6409) | 443797..444552 | - | 756 | WP_246790288 | P-type conjugative transfer protein TrbJ | virB5 |
| AA2016_RS31895 (AA2016_6410) | 444578..447055 | - | 2478 | WP_067969586 | conjugal transfer protein TrbE | virb4 |
| AA2016_RS31900 (AA2016_6411) | 447068..447367 | - | 300 | WP_067969590 | conjugal transfer protein TrbD | virB3 |
| AA2016_RS31905 (AA2016_6412) | 447360..447731 | - | 372 | WP_067969593 | TrbC/VirB2 family protein | virB2 |
| AA2016_RS31910 (AA2016_6413) | 447721..448692 | - | 972 | WP_067969596 | P-type conjugative transfer ATPase TrbB | virB11 |
| AA2016_RS35730 | 449305..449571 | - | 267 | WP_347400140 | transposase | - |
| AA2016_RS35525 | 449568..449831 | - | 264 | WP_249926038 | transposase | - |
Host bacterium
| ID | 9291 | GenBank | NZ_CP015007 |
| Plasmid name | pAA02 | Incompatibility group | - |
| Plasmid size | 451547 bp | Coordinate of oriT [Strand] | 155984..156023 [+] |
| Host baterium | Aminobacter aminovorans strain KCTC 2477 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |