Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   108825
Name   oriT_PS_Mu_10_2021|pESI in_silico
Organism   Salmonella enterica subsp. enterica serovar Muenchen strain PS_Mu_10_2021
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP120328 (20032..20120 [+], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_PS_Mu_10_2021|pESI
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   5912 GenBank   WP_001299212
Name   nikB_P1825_RS23875_PS_Mu_10_2021|pESI insolico UniProt ID   A0A5T4AK12
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103956.40 Da        Isoelectric Point: 7.2642

>WP_001299212.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWINFYGVTCFHNCTSLETAAADMEYIALQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVSDWLSLHRRLAEDGLYLSQMDGK
YLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWLPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A5T4AK12


Auxiliary protein

ID   2932 GenBank   WP_001283947
Name   WP_001283947_PS_Mu_10_2021|pESI insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   6364 GenBank   WP_001289276
Name   trbC_P1825_RS23870_PS_Mu_10_2021|pESI insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 226..14654

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1825_RS23795 (P1825_23795) 54..242 + 189 WP_001277255 putative conjugal transfer protein TraS -
P1825_RS23800 (P1825_23800) 226..1026 + 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
P1825_RS23805 (P1825_23805) 1116..4160 + 3045 WP_001024780 IncI1-type conjugal transfer protein TraU traU
P1825_RS23810 (P1825_23810) 4160..4774 + 615 WP_000337400 IncI1-type conjugal transfer protein TraV traV
P1825_RS23815 (P1825_23815) 4741..5943 + 1203 WP_001189156 IncI1-type conjugal transfer protein TraW traW
P1825_RS23820 (P1825_23820) 5972..6556 + 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
P1825_RS23825 (P1825_23825) 6653..8821 + 2169 WP_000698357 DotA/TraY family protein traY
P1825_RS23830 (P1825_23830) 8895..9545 + 651 WP_001178506 plasmid IncI1-type surface exclusion protein ExcA -
P1825_RS23835 (P1825_23835) 9617..9826 - 210 WP_000062603 HEAT repeat domain-containing protein -
P1825_RS23840 (P1825_23840) 10218..10394 + 177 WP_001054904 hypothetical protein -
P1825_RS23845 (P1825_23845) 10459..10554 - 96 WP_001303310 DinQ-like type I toxin DqlB -
P1825_RS23850 (P1825_23850) 11186..11779 + 594 WP_283902961 hypothetical protein -
P1825_RS23855 (P1825_23855) 11772..12095 + 324 WP_259382950 hypothetical protein -
P1825_RS23860 (P1825_23860) 12357..13565 + 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
P1825_RS23865 (P1825_23865) 13584..14654 + 1071 WP_000151577 IncI1-type conjugal transfer protein TrbB trbB
P1825_RS23870 (P1825_23870) 14647..16938 + 2292 WP_001289276 F-type conjugative transfer protein TrbC -

Region 2: 263590..285069

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1825_RS25075 (P1825_25075) 258652..259719 + 1068 WP_001360287 type IV pilus biogenesis lipoprotein PilL -
P1825_RS25080 (P1825_25080) 259719..260156 + 438 WP_000539807 type IV pilus biogenesis protein PilM -
P1825_RS25085 (P1825_25085) 260170..261852 + 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
P1825_RS25090 (P1825_25090) 261845..263140 + 1296 WP_001545757 type 4b pilus protein PilO2 -
P1825_RS25095 (P1825_25095) 263127..263579 + 453 WP_001247331 type IV pilus biogenesis protein PilP -
P1825_RS25100 (P1825_25100) 263590..265143 + 1554 WP_000362206 ATPase, T2SS/T4P/T4SS family virB11
P1825_RS25105 (P1825_25105) 265156..266241 + 1086 WP_001208802 type II secretion system F family protein -
P1825_RS25110 (P1825_25110) 266258..266872 + 615 WP_000908226 type 4 pilus major pilin -
P1825_RS25115 (P1825_25115) 266882..267442 + 561 WP_000014005 lytic transglycosylase domain-containing protein virB1
P1825_RS25120 (P1825_25120) 267427..268083 + 657 WP_001193549 A24 family peptidase -
P1825_RS25125 (P1825_25125) 268083..269375 + 1293 WP_001350014 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
P1825_RS25130 (P1825_25130) 269431..269556 - 126 Protein_270 RNA-guided endonuclease TnpB family protein -
P1825_RS25135 (P1825_25135) 269764..270588 + 825 WP_001238942 conjugal transfer protein TraE traE
P1825_RS25140 (P1825_25140) 270674..271876 + 1203 WP_000976348 conjugal transfer protein TraF -
P1825_RS25145 (P1825_25145) 271936..272520 + 585 WP_000977522 histidine phosphatase family protein -
P1825_RS25150 (P1825_25150) 272915..273373 + 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
P1825_RS25155 (P1825_25155) 273370..274188 + 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
P1825_RS25160 (P1825_25160) 274185..275333 + 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
P1825_RS25165 (P1825_25165) 275330..275620 + 291 WP_001299214 hypothetical protein traK
P1825_RS25170 (P1825_25170) 275635..276186 + 552 WP_000014584 phospholipase D family protein -
P1825_RS25175 (P1825_25175) 276276..280043 + 3768 WP_001141542 LPD7 domain-containing protein -
P1825_RS25180 (P1825_25180) 280061..280408 + 348 WP_001055900 conjugal transfer protein traL
P1825_RS25185 (P1825_25185) 280405..281097 + 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
P1825_RS25190 (P1825_25190) 281108..282091 + 984 WP_001191878 IncI1-type conjugal transfer protein TraN traN
P1825_RS25195 (P1825_25195) 282094..283383 + 1290 WP_001271997 conjugal transfer protein TraO traO
P1825_RS25200 (P1825_25200) 283383..284087 + 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
P1825_RS25205 (P1825_25205) 284087..284614 + 528 WP_001055569 conjugal transfer protein TraQ traQ
P1825_RS25210 (P1825_25210) 284665..285069 + 405 WP_000086961 IncI1-type conjugal transfer protein TraR traR

Host bacterium

ID   9260 GenBank   NZ_CP120328
Plasmid name   PS_Mu_10_2021|pESI Incompatibility group   IncFIB
Plasmid size   285079 bp Coordinate of oriT [Strand]   20032..20120 [+]
Host baterium   Salmonella enterica subsp. enterica serovar Muenchen strain PS_Mu_10_2021

Cargo genes

Drug resistance gene   dfrA14, ant(3'')-Ia, qacE, sul1, tet(A)
Virulence gene   -
Metal resistance gene   merE, merD, merA, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -