Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   108823
Name   oriT_PS_Mu_2_2021|pESI in_silico
Organism   Salmonella enterica subsp. enterica serovar Muenchen strain PS_Mu_2_2021
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP120348 (230834..230922 [+], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_PS_Mu_2_2021|pESI
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   5910 GenBank   WP_001299212
Name   nikB_P1828_RS24845_PS_Mu_2_2021|pESI insolico UniProt ID   A0A5T4AK12
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103956.40 Da        Isoelectric Point: 7.2642

>WP_001299212.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWINFYGVTCFHNCTSLETAAADMEYIALQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVSDWLSLHRRLAEDGLYLSQMDGK
YLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWLPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A5T4AK12


Auxiliary protein

ID   2931 GenBank   WP_001283947
Name   WP_001283947_PS_Mu_2_2021|pESI insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   6362 GenBank   WP_001289276
Name   trbC_P1828_RS24840_PS_Mu_2_2021|pESI insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 189312..225456

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1828_RS24630 (P1828_24630) 184374..185441 + 1068 WP_001360287 type IV pilus biogenesis lipoprotein PilL -
P1828_RS24635 (P1828_24635) 185441..185878 + 438 WP_000539807 type IV pilus biogenesis protein PilM -
P1828_RS24640 (P1828_24640) 185892..187574 + 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
P1828_RS24645 (P1828_24645) 187567..188862 + 1296 WP_001545757 type 4b pilus protein PilO2 -
P1828_RS24650 (P1828_24650) 188849..189301 + 453 WP_001247331 type IV pilus biogenesis protein PilP -
P1828_RS24655 (P1828_24655) 189312..190865 + 1554 WP_000362206 ATPase, T2SS/T4P/T4SS family virB11
P1828_RS24660 (P1828_24660) 190878..191963 + 1086 WP_001208802 type II secretion system F family protein -
P1828_RS24665 (P1828_24665) 191980..192594 + 615 WP_000908226 type 4 pilus major pilin -
P1828_RS24670 (P1828_24670) 192604..193164 + 561 WP_000014005 lytic transglycosylase domain-containing protein virB1
P1828_RS24675 (P1828_24675) 193149..193805 + 657 WP_001193549 A24 family peptidase -
P1828_RS24680 (P1828_24680) 193805..195097 + 1293 WP_001350014 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
P1828_RS24685 (P1828_24685) 195153..195278 - 126 Protein_182 RNA-guided endonuclease TnpB family protein -
P1828_RS24690 (P1828_24690) 195486..196310 + 825 WP_001238942 conjugal transfer protein TraE traE
P1828_RS24695 (P1828_24695) 196396..197598 + 1203 WP_000976348 conjugal transfer protein TraF -
P1828_RS24700 (P1828_24700) 197658..198242 + 585 WP_000977522 histidine phosphatase family protein -
P1828_RS24705 (P1828_24705) 198637..199095 + 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
P1828_RS24710 (P1828_24710) 199092..199910 + 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
P1828_RS24715 (P1828_24715) 199907..201055 + 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
P1828_RS24720 (P1828_24720) 201052..201342 + 291 WP_001299214 hypothetical protein traK
P1828_RS24725 (P1828_24725) 201357..201908 + 552 WP_000014584 phospholipase D family protein -
P1828_RS24730 (P1828_24730) 201998..205765 + 3768 WP_001141542 LPD7 domain-containing protein -
P1828_RS24735 (P1828_24735) 205793..206131 + 339 WP_349632730 conjugal transfer protein -
P1828_RS24740 (P1828_24740) 206128..206820 + 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
P1828_RS24745 (P1828_24745) 206831..207815 + 985 Protein_194 IncI1-type conjugal transfer protein TraN -
P1828_RS24750 (P1828_24750) 207818..209107 + 1290 WP_001271997 conjugal transfer protein TraO traO
P1828_RS24755 (P1828_24755) 209107..209811 + 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
P1828_RS24760 (P1828_24760) 209811..210338 + 528 WP_001055569 conjugal transfer protein TraQ traQ
P1828_RS24765 (P1828_24765) 210389..210793 + 405 WP_000086961 IncI1-type conjugal transfer protein TraR traR
P1828_RS24770 (P1828_24770) 210857..211045 + 189 WP_001277255 putative conjugal transfer protein TraS -
P1828_RS24775 (P1828_24775) 211029..211829 + 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
P1828_RS24780 (P1828_24780) 211919..214963 + 3045 WP_001024780 IncI1-type conjugal transfer protein TraU traU
P1828_RS24785 (P1828_24785) 214963..215577 + 615 WP_000337400 IncI1-type conjugal transfer protein TraV traV
P1828_RS24790 (P1828_24790) 215544..216746 + 1203 WP_001189156 IncI1-type conjugal transfer protein TraW traW
P1828_RS24795 (P1828_24795) 216775..217359 + 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
P1828_RS24800 (P1828_24800) 217456..219624 + 2169 WP_000698357 DotA/TraY family protein traY
P1828_RS24805 (P1828_24805) 219698..220348 + 651 WP_001178506 plasmid IncI1-type surface exclusion protein ExcA -
P1828_RS24810 (P1828_24810) 220420..220629 - 210 WP_000062603 HEAT repeat domain-containing protein -
P1828_RS24815 (P1828_24815) 221021..221197 + 177 WP_001054904 hypothetical protein -
P1828_RS24820 (P1828_24820) 221262..221357 - 96 WP_001303310 DinQ-like type I toxin DqlB -
P1828_RS24825 (P1828_24825) 221989..222897 + 909 WP_000055084 hypothetical protein -
P1828_RS24830 (P1828_24830) 223159..224367 + 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
P1828_RS24835 (P1828_24835) 224386..225456 + 1071 WP_000151577 IncI1-type conjugal transfer protein TrbB trbB
P1828_RS24840 (P1828_24840) 225449..227740 + 2292 WP_001289276 F-type conjugative transfer protein TrbC -

Host bacterium

ID   9258 GenBank   NZ_CP120348
Plasmid name   PS_Mu_2_2021|pESI Incompatibility group   IncFIB
Plasmid size   285075 bp Coordinate of oriT [Strand]   230834..230922 [+]
Host baterium   Salmonella enterica subsp. enterica serovar Muenchen strain PS_Mu_2_2021

Cargo genes

Drug resistance gene   ant(3'')-Ia, qacE, sul1, tet(A), dfrA14
Virulence gene   -
Metal resistance gene   merE, merD, merA, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -