Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 108759 |
| Name | oriT_p14523A  |
| Organism | Salmonella sp. SJTUF14523 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP074429 (85417..85505 [-], 89 nt) |
| oriT length | 89 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 89 nt
>oriT_p14523A
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Auxiliary protein
| ID | 2898 | GenBank | WP_001283947 |
| Name | WP_001283947_p14523A |
UniProt ID | A0A142CMC2 |
| Length | 110 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 110 a.a. Molecular weight: 12613.57 Da Isoelectric Point: 10.7463
>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG
Protein domains
No domain identified.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A142CMC2 |
T4CP
| ID | 6309 | GenBank | WP_012783931 |
| Name | trbC_KFD69_RS22650_p14523A |
UniProt ID | _ |
| Length | 763 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 763 a.a. Molecular weight: 86911.02 Da Isoelectric Point: 6.7713
>WP_012783931.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILTAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILTAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 5021..43923
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KFD69_RS22650 (KFD69_22635) | 2737..5028 | - | 2292 | WP_012783931 | F-type conjugative transfer protein TrbC | - |
| KFD69_RS22655 (KFD69_22640) | 5021..6091 | - | 1071 | WP_012783932 | IncI1-type conjugal transfer protein TrbB | trbB |
| KFD69_RS22660 (KFD69_22645) | 6110..7318 | - | 1209 | WP_001703845 | IncI1-type conjugal transfer protein TrbA | trbA |
| KFD69_RS22665 (KFD69_22650) | 7625..8404 | - | 780 | WP_275450201 | protein FinQ | - |
| KFD69_RS22670 (KFD69_22655) | 9031..9183 | + | 153 | WP_001387489 | Hok/Gef family protein | - |
| KFD69_RS22675 (KFD69_22660) | 9255..9506 | - | 252 | WP_001291964 | hypothetical protein | - |
| KFD69_RS24035 | 10007..10102 | + | 96 | WP_000609148 | DinQ-like type I toxin DqlB | - |
| KFD69_RS22685 (KFD69_22670) | 10167..10343 | - | 177 | WP_001054900 | hypothetical protein | - |
| KFD69_RS22690 (KFD69_22675) | 10735..10944 | + | 210 | WP_000062603 | HEAT repeat domain-containing protein | - |
| KFD69_RS22695 (KFD69_22680) | 11016..11678 | - | 663 | WP_012783935 | plasmid IncI1-type surface exclusion protein ExcA | - |
| KFD69_RS22700 (KFD69_22685) | 11749..13917 | - | 2169 | WP_015508354 | DotA/TraY family protein | traY |
| KFD69_RS22705 (KFD69_22690) | 14014..14598 | - | 585 | WP_012783937 | conjugal transfer protein TraX | - |
| KFD69_RS22710 (KFD69_22695) | 14627..15829 | - | 1203 | WP_012783938 | IncI1-type conjugal transfer protein TraW | traW |
| KFD69_RS22715 (KFD69_22700) | 15796..16410 | - | 615 | WP_000337394 | IncI1-type conjugal transfer protein TraV | traV |
| KFD69_RS22720 (KFD69_22705) | 16410..19454 | - | 3045 | WP_012783939 | IncI1-type conjugal transfer protein TraU | traU |
| KFD69_RS22725 (KFD69_22710) | 19544..20344 | - | 801 | WP_001164788 | IncI1-type conjugal transfer protein TraT | traT |
| KFD69_RS22730 (KFD69_22715) | 20328..20516 | - | 189 | WP_001277255 | putative conjugal transfer protein TraS | - |
| KFD69_RS22735 (KFD69_22720) | 20580..20984 | - | 405 | WP_000086965 | IncI1-type conjugal transfer protein TraR | traR |
| KFD69_RS22740 (KFD69_22725) | 21035..21562 | - | 528 | WP_001055569 | conjugal transfer protein TraQ | traQ |
| KFD69_RS22745 (KFD69_22730) | 21562..22266 | - | 705 | WP_001493811 | IncI1-type conjugal transfer protein TraP | traP |
| KFD69_RS22750 (KFD69_22735) | 22266..23555 | - | 1290 | WP_011264042 | conjugal transfer protein TraO | traO |
| KFD69_RS22755 (KFD69_22740) | 23558..24541 | - | 984 | WP_001191880 | IncI1-type conjugal transfer protein TraN | traN |
| KFD69_RS22760 (KFD69_22745) | 24552..25244 | - | 693 | WP_000138552 | DotI/IcmL family type IV secretion protein | traM |
| KFD69_RS22765 (KFD69_22750) | 25241..25588 | - | 348 | WP_001055900 | conjugal transfer protein | traL |
| KFD69_RS22770 (KFD69_22755) | 25606..29373 | - | 3768 | WP_012783940 | LPD7 domain-containing protein | - |
| KFD69_RS22775 (KFD69_22760) | 29463..30014 | - | 552 | WP_012783942 | phospholipase D family protein | - |
| KFD69_RS22780 (KFD69_22765) | 30029..30319 | - | 291 | WP_001314267 | hypothetical protein | traK |
| KFD69_RS22785 (KFD69_22770) | 30316..31464 | - | 1149 | WP_001024971 | plasmid transfer ATPase TraJ | virB11 |
| KFD69_RS22790 (KFD69_22775) | 31461..32279 | - | 819 | WP_000646097 | IncI1-type conjugal transfer lipoprotein TraI | traI |
| KFD69_RS22795 (KFD69_22780) | 32276..32734 | - | 459 | WP_001079808 | IncI1-type conjugal transfer lipoprotein TraH | - |
| KFD69_RS22800 (KFD69_22785) | 33128..33712 | - | 585 | WP_000977522 | histidine phosphatase family protein | - |
| KFD69_RS22805 (KFD69_22790) | 33772..34974 | - | 1203 | WP_000976353 | conjugal transfer protein TraF | - |
| KFD69_RS22810 (KFD69_22795) | 35060..35884 | - | 825 | WP_001238932 | conjugal transfer protein TraE | traE |
| KFD69_RS22815 (KFD69_22800) | 36035..37189 | - | 1155 | WP_001139955 | site-specific integrase | - |
| KFD69_RS22820 (KFD69_22805) | 37188..37463 | + | 276 | WP_000958118 | hypothetical protein | - |
| KFD69_RS22825 (KFD69_22810) | 37460..37708 | - | 249 | WP_001349157 | hypothetical protein | - |
| KFD69_RS22830 (KFD69_22815) | 38089..39417 | - | 1329 | WP_001496394 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| KFD69_RS22835 (KFD69_22820) | 39417..40073 | - | 657 | WP_213630803 | prepilin peptidase | - |
| KFD69_RS22840 (KFD69_22825) | 40058..40618 | - | 561 | WP_000014116 | lytic transglycosylase domain-containing protein | virB1 |
| KFD69_RS22845 (KFD69_22830) | 40628..41242 | - | 615 | WP_001542527 | type 4 pilus major pilin | - |
| KFD69_RS22850 (KFD69_22835) | 41260..42357 | - | 1098 | WP_001492931 | type II secretion system F family protein | - |
| KFD69_RS22855 (KFD69_22840) | 42370..43923 | - | 1554 | WP_001492932 | ATPase, T2SS/T4P/T4SS family | virB11 |
| KFD69_RS22860 (KFD69_22845) | 43934..44386 | - | 453 | WP_012783945 | type IV pilus biogenesis protein PilP | - |
| KFD69_RS22865 (KFD69_22850) | 44373..45668 | - | 1296 | WP_012783946 | type 4b pilus protein PilO2 | - |
| KFD69_RS22870 (KFD69_22855) | 45661..47343 | - | 1683 | WP_000748143 | PilN family type IVB pilus formation outer membrane protein | - |
| KFD69_RS22875 (KFD69_22860) | 47357..47794 | - | 438 | WP_000539807 | type IV pilus biogenesis protein PilM | - |
| KFD69_RS22880 (KFD69_22865) | 47794..48861 | - | 1068 | WP_012783947 | type IV pilus biogenesis lipoprotein PilL | - |
Host bacterium
| ID | 9194 | GenBank | NZ_CP074429 |
| Plasmid name | p14523A | Incompatibility group | IncI1 |
| Plasmid size | 85862 bp | Coordinate of oriT [Strand] | 85417..85505 [-] |
| Host baterium | Salmonella sp. SJTUF14523 |
Cargo genes
| Drug resistance gene | blaCTX-M-101 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrVA2 |