Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 108653
• Name: oriT_pUL021B
• Organism: Lactococcus lactis subsp. lactis strain UL021
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP070265 (19227..19254 [-], 28 nt)
• oriT length: 28 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 28 nt

>oriT_pUL021B
ACCACCCAATTTTGGAGTGGTGTGTAAG

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_LLUL021_RS13205_pUL021B

• ID: 5772
• GenBank: WP_137016247
• Name: Relaxase_LLUL021_RS13205_pUL021B
• UniProt ID: _
• Length: 505 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 505 a.a.
• Molecular weight: 59157.84 Da
• Isoelectric Point: 7.6002

>WP_137016247.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Lactococcus]
MTVIYMPKNSNGTVHSSKDLNQLINYVMNPEKTNDFEYISGQNILNIHSACDEMLATRELAIFTGKSPKK
NEIFGYHFVQSFSPDDNLTPAQVHEIGCKTMKEYLGNSAEFIIATHTDKPHLHNHIVLNATNPLTLNKFH
QNKTDLERLKEISDRISKEYGCKIIDRPEQKLGNSHKNYLVYLAKNSYRKEIKNKLEFLMNHSHTWEDFK
TKASALNLKVDDTKKYTTYLLEGSEQTKKIRDRSLKNDKFLKENLKERIEKNTIGYSVEEVVKLWNDKES
IQEKSQEKEISILVEDWQIEKETEKYLYVTIDTALDKEATIKIPARCVDKLENGDYQVFIKNKDRFSYQD
KKSPENNKIMYGTTVAKNLQRQSGNVPLYSDNANIKLKQVFHEFDFLISQGLGFDRSFETIGKDLKATYH
ETEKQLDKLDTKIVEYVEKNKSLDYVDTSIRDTIKNLKLERDDLRDTLYKVDKNIQYYQKSEQRLEAYQK
NQSPKHKARDDDFEI

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (17-261)

Protein structure

No available structure.

T4CP

t4cp2_LLUL021_RS13275_pUL021B

• ID: 6184
• GenBank: WP_137016068
• Name: t4cp2_LLUL021_RS13275_pUL021B
• UniProt ID: _
• Length: 611 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 611 a.a.
• Molecular weight: 70655.97 Da
• Isoelectric Point: 6.2797

>WP_137016068.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Lactococcus]
MVKKTKIILLSILAIFIGVFFNHFTYYFLLEKSDGIYKLINCLEKVTSSPYLFFSIQTYPLLLFLLGLFI
GGLILILSLGTGKYRQGKEYGSATWATKGQLKPFQAKPPKIKKGETPPEEDMNIILSKDIKMSLPTHSMP
FKYQRNKNILLIAGAGAGKTEMFVKPNLSQLHSSYVVTDPKGKLLHETGKMMSEHGYTIKCFNVNDFSNS
NKFNPFKYIRDEVTLKRVIQCIIDATNGENSKKGEPFWDKSEELLLSSLFSMLYYNYKDEVETNPDKEIE
LPKLYEASDLIRLLNRTDEETPSPLELAFEEFEEDFGSDNYAVTQFNSFKNYKGKTRSSVLAIATARFSM
FDLKDIREVLDDDELDIDSWIDKKTIVYLPIPDMDTTFNFLTTMIFVLAFRTLEYKIDNIPPKNEYLHVR
FILDEFANLGKIPNIKEALAVFRSREMSINIILQNLNQLRALYKDDWQSFVGNCDTIMYLSGSTEPETEK
FFSERAGKETINMRKQTENRGGQGSYSINHDVLGRDLITKDEVNRLPRDECLISISSMPMYKGKKFKFNG
HKRSSEWSRSSNDENWFDFKPVHKKKIVNVVEEETLKDDEYVENIYSQFND

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (147-558)

Protein structure

No available structure.

Host bacterium

• ID: 9088
• GenBank: NZ_CP070265
• Plasmid name: pUL021B
• Incompatibility group: -
• Plasmid size: 62446 bp
• Coordinate of oriT [Strand]: 19227..19254 [-]
• Host baterium: Lactococcus lactis subsp. lactis strain UL021

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21