Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 108652
• Name: oriT_pSDGJQ5
• Organism: Enterococcus faecium strain SDGJQ5
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP038175 (24782..24881 [-], 100 nt)
• oriT length: 100 nt
• IRs (inverted repeats): 13..18, 32..37 (AAAGGG..CCCTTT)
• Location of nic site: 94..95
• Conserved sequence flanking the nic site: GCTTGCAGTA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 100 nt

>oriT_pSDGJQ5
CCGAAGGGGATGAAAGGGAACGTGTAACTTGCCCTTTTCCAAGCAACTTTGTCATACAAGACACTTCGTTATTGTATGACAAAGTGAAAGCTTGCAGTAT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_E3T52_RS13285_pSDGJQ5

• ID: 5771
• GenBank: WP_231412101
• Name: Relaxase_E3T52_RS13285_pSDGJQ5
• UniProt ID: _
• Length: 329 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 329 a.a.
• Molecular weight: 38641.82 Da
• Isoelectric Point: 10.1186

>WP_231412101.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterococcus]
MTQSFALDELSPKNTQDWQRANDLGVALAEKLYPQHQTAVYTHLDGQNHVLHNHILVNKVNLETGKKLRE
VPGKTVEKARVINDRLAQQQHWRILPPPQERFSETEQDLVAKQKYSYMDDLRTRINEAMVDISVRSYKDL
RNILEQKSIIFQERVQSLSYAFLDVNNKKRRAKGARLGTDFEKETIFNELENRARQQQPRPIEPVKQREL
ATTRLMQDTQQTEPSLERRESTTRGIEQQLEQRKPGIARLIDTIQHLRDQLPGITQLVKKHLTYAKEALF
DAFEKRFSFDMAQHKQVQRKKIQQDLEQRTQEQAMPKFQTPTQDRGLSL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (2-192)

Protein structure

No available structure.

Host bacterium

• ID: 9087
• GenBank: NZ_CP038175
• Plasmid name: pSDGJQ5
• Incompatibility group: -
• Plasmid size: 30457 bp
• Coordinate of oriT [Strand]: 24782..24881 [-]
• Host baterium: Enterococcus faecium strain SDGJQ5

Cargo genes

• Drug resistance gene: poxtA
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA1, AcrIIA21