Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   108525
Name   oriT_pK666_mcr-10 in_silico
Organism   Enterobacter roggenkampii strain K666
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP095171 (120680..120728 [+], 49 nt)
oriT length   49 nt
IRs (inverted repeats)      6..11, 18..23  (GCAAAA..TTTTGC)
Location of nic site      32..33
Conserved sequence flanking the
  nic site    		  GGTGTGGTGA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 49 nt

>oriT_pK666_mcr-10
AATCAGCAAAACTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   5697 GenBank   WP_249489027
Name   traI_MUY33_RS24345_pK666_mcr-10 insolico UniProt ID   _
Length   1747 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1747 a.a.        Molecular weight: 189134.33 Da        Isoelectric Point: 6.0230

>WP_249489027.1 conjugative transfer relaxase/helicase TraI [Enterobacter roggenkampii]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMGERWAGKGAEALGLSGGVDQKVFTKVLEGRLPDGSDLSRTQ
DGANKHRPGYDFTFSAPKSVSVMAMLGGDKRLIEAHNRAVETAIKQIEAMASTRVMTEGRSETQLTGNLV
MALFNHDTSRDQEPQLHTHAVVANATQHGDEWRTLSSDKVGKTGFIENIYANQIAFGRLYRAALKDDVAA
MGYETETVGKHGMWELKGVPTEPYSSRSRTISEAVGDDASLKSRDVAALDTRQSKQKVDPEQRMAEWMQT
LKETGFDIKAYREAADLRVVQGNIPVTAPEAIDINSSVGQAIAMLSDRRARFTYSELLATTLGQLPARSG
MVEMARDGIDAAIKNEQLIPLDKEKGLFTSNIHVLDELSVSAMTRDLQRTGQADIFPDKSVPRSRSYSDA
VSVLAQDRPPVAIISGQGGAAGQRERVAELTMMAREQGRDVQIIAADRRSRSALMQDERLSGEHITDRRE
LTEGMTFTPGSTLIVDQGEKLTLKETLTLLDGALRHNVQLLIADSGQRTGTGSALTVMKETGVSTLAWQG
GEKTQVSVISEPDRRQRYGRLAADFARSVRAGEHSVAQVSGPREQAVLAGAVREALKAEKVLGEREVSIT
TLEPVWLDSRHQQVRDHYREGMVMERWNAEERSRERFVIDRVTGRNNSLTLRNAQGETRVTRLSELNSSW
SLYRTGTLQVAEGDRLAVLGQAQGTRLKGGDTVTVKALGEAGLVVSLSGRKADVVLPAGDSPFTAAKVGQ
GWVESPGRSVSDSATVFASLSQRELDNTTLNKLALSGRAVQLYSAQTAQKTTEKLSRQSAWSVVTSQVKE
AAGKDALGDALAHQKAGLHTPEQQAIHLAIPVLEGNGLAFTKPQLMAAAKEFEQDCLSLQAIEKEADRQV
RSGDLLSVPVSPGNGLQLLVSRQSYNAEKSIIRHVLEGKEAVTPLMDKVPDAQLAGLTDGQQNATRMILE
SPDRFTLVQGYAGVGKTTQFRAVMSAIGTLPEREQLRVIGVAPTHRAVSEMRDAGVPETQTLAAFIHDTQ
QQLRGGERPDFSNVLFLVDESSMVGNADMAKAYSLIAGGGGRAVSSGDTDQLQSIAPGQPFRLLQKRSAI
DVAVMQEIVRQTPELKPAVYSLINRDIGSALTTIERVAPAQVPRRADAWQPDSSVMEFSREREEAIAKAM
AAGDLLPGGEPATLLEAIVKDYTGRTPEAQAQTIVITALNADRRQVNAMIHDARQDAGEVGEKEVTLPVL
TPANIRDGDLRRMATWEASRDSLVLLDSTYYSIEALDGEAHLVTLKDAQGNTRSLSPAQAATEGVTLYRQ
DTITVSEGDRMRFSKSDNERGFVANSVWSVSEIKGDSVTLSDGKQTRTLTPSAEQAEQHIDLAYAVTVEG
SQGASEPFSISLQGTDGGRKQMVSFESAYVALSRMKQHAQVYTDNRDKWVAAMEKSQAKSTAHDILEPRG
DRAVANAARLTATAKALGEVPAGRAALRQAGLQPEGSMAKFISPGRKYPQPHVALPAFDRNGRQAGVWLS
ALTSGDGQLKGLAGEGRVMGSGDAAFAGLQASRNGESLLARDMEEGVRLARENPQSGVVVRLAGEARPWN
PGAITGGRVWADGLLDGTGTQPGVTVPQEVLAQQAREAEVQRELDKRAEEAVREMARSGDGTAGGADVAV
RDVVRDMERIKETPASPLTLPDAPEERRRQREAVSQVVRENVQRDRLQQMERETVRDLEREKTLGGD

  Protein domains


Predicted by InterproScan.

DNA helicase TraI (TraI_C)

(1430-1551)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(573-625)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(631-710)

AAA domain (AAA_30)

(967-1152)

TrwC relaxase (TrwC)

(10-283)


  Protein structure



No available structure.




T4CP

ID   6077 GenBank   WP_249489028
Name   traD_MUY33_RS24350_pK666_mcr-10 insolico UniProt ID   _
Length   715 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 715 a.a.        Molecular weight: 80144.53 Da        Isoelectric Point: 5.1609

>WP_249489028.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacter cloacae complex]
MSFNAKDMTQGGQIASMRIRMFSQIANIIVYCLVILFGIIVAITLSVKLTWQTFVNGMVYYWCQTLAPMR
DIFKSQPVYTIRYYGHELKYNAQQILQDKYIQWCADQVWTAFILAAIIAAVMCVITFFVTTWILGHQGKQ
QSEDEITGGRQLTDDPKAVAKMLRRDGLASDIKIGELPLIKDSEIQNFCLHGTVGAGKSEVIRRLMNYAR
QRGDMVIVYDRSCEFVKSYYDPATDKILNPLDARCAAWDLWRECLTQPDFDNVSNTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDKDRSYSKLVDTLLSIKIEKLRSYLKNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIENNGESFTIRDWMRGVREDGKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVIGIQSYAQLEDIYGQKPAETLFDVLNTRAFFRSPSRNIA
EFAAGEIGEKEILKASEQYSYGVDPVRDGTSVGKDMERVTLVSYSDVQTLPDLSCYVTLPGPYPAVKMAL
KYQQRPKVAPEFIPRTIDGKAEERLNAALAAREHESRSIAMLFEPDADVVAPEGGAENAGQPQQPATAPR
AADTASVTGAAGTGGVEPELKTKAEEAEQLPPGISESGEVVDMAAWEAWQAEQDELSPQERQRREEVNIN
VHRAPEKDLEPGGYI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

F sex factor protein N terminal (TraD_N)

(32-128)

  Protein structure



No available structure.



ID   6078 GenBank   WP_058654351
Name   traC_MUY33_RS24420_pK666_mcr-10 insolico UniProt ID   _
Length   876 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 876 a.a.        Molecular weight: 98855.54 Da        Isoelectric Point: 5.7676

>WP_058654351.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacteriaceae]
MSNPLDTVTRTVNSLLTALKMPDASSEANDVLGQMRFPQFSRVLPYRDYDAETGLFMNEKTMGFMLEAQP
INGANETIVSSLENLMRTKLPREVPVAVHLVSSHLVGQDIEYGLRELSWTGEKADTFNAITRAYYLRAAE
EGFPLPDHLNLPLTLRNYRVYISCCVPRKRGSKASVMEMENQVKIIRASLQGAQIATRSVDADAFIRIAG
EMINHNPDALYPTERELDPLKDLNYQCVDDTFDLKVKADYMTLGLRGNGRNSTARIMNFQLTKNPELAFL
WNMADNYSNLLNPELSVSCPFILTLVLSVEDQVKAQNEANLKFMDLEKKSKTSYAKFFPGVAQEAKEWGD
LRQRLSSGQSSLVSYFLNVTLFCRDDQETALKVEQDVLNSFRKNGFELISPRFNHMRNFLVNLPFMAGGG
LFKQLRESGVLQRAESFNVANLMPVVADSPLATSGLLAPTYRNQLAFIDLFSRGMGNTNFNMAVCGTSGA
GKTGLIQPLIRSVIDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLKFNPFANVTDDTIDEMAERLRDQL
SVMASPNGNLDEVHEGLLLKAVKATWLTKKNQARIDDVVDYLMMARDSEEYSGSPTIRSRLDEMVVLLTQ
YTRDGVYGSYFNSDEPSLKDEARMVVLELGGLEDRPSLLVAVMFSLILYIENRMYRTPRSLKKLNVIDEG
WRLLDFKNNKVGAFIEKGYRTARRHTGSYITITQNIVDFDSETASSAARAAWGNSSYKIILKQSAKEFAK
YNQLYPDQYSQLQRDMIKGFGSAKDNWFSSFMLSVEHLTSWHRLFVDPLSRAMYSSDGPDFEFLQQRRRE
GLSIHEAVWELAWKKSGPEMAALERWLHEHEGKTIS

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(289-446)

TraG P-loop domain (P-loop_TraG)

(470-765)

TraC protein (TraC_F_IV)

(38-276)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 94740..121286

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUY33_RS24350 (MUY33_24350) 94740..96887 - 2148 WP_249489028 type IV conjugative transfer system coupling protein TraD virb4
MUY33_RS24355 (MUY33_24355) 97367..98347 + 981 WP_044713288 IS5-like element IS5 family transposase -
MUY33_RS24360 (MUY33_24360) 98494..98991 - 498 WP_249489029 hypothetical protein -
MUY33_RS24365 (MUY33_24365) 99010..101832 - 2823 WP_249489030 conjugal transfer mating-pair stabilization protein TraG traG
MUY33_RS24370 (MUY33_24370) 101832..103208 - 1377 WP_086538482 conjugal transfer pilus assembly protein TraH traH
MUY33_RS24375 (MUY33_24375) 103205..103750 - 546 WP_086551403 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
MUY33_RS24380 (MUY33_24380) 103740..103988 - 249 WP_008324126 type-F conjugative transfer system pilin chaperone TraQ -
MUY33_RS24385 (MUY33_24385) 104005..104742 - 738 WP_077138956 type-F conjugative transfer system pilin assembly protein TraF traF
MUY33_RS24390 (MUY33_24390) 104785..104973 - 189 WP_077138957 hypothetical protein -
MUY33_RS24395 (MUY33_24395) 105005..106885 - 1881 WP_077138958 type-F conjugative transfer system mating-pair stabilization protein TraN traN
MUY33_RS24400 (MUY33_24400) 106882..107511 - 630 WP_060446832 type-F conjugative transfer system pilin assembly protein TrbC trbC
MUY33_RS24405 (MUY33_24405) 107528..108514 - 987 WP_032637387 conjugal transfer pilus assembly protein TraU traU
MUY33_RS24410 (MUY33_24410) 108517..109164 - 648 WP_077138960 type-F conjugative transfer system protein TraW traW
MUY33_RS24415 (MUY33_24415) 109164..109511 - 348 WP_060446830 type-F conjugative transfer system protein TrbI -
MUY33_RS24420 (MUY33_24420) 109508..112138 - 2631 WP_058654351 type IV secretion system protein TraC virb4
MUY33_RS24425 (MUY33_24425) 112131..112694 - 564 WP_200923953 conjugal transfer pilus-stabilizing protein TraP -
MUY33_RS24430 (MUY33_24430) 112684..113088 - 405 WP_249489031 hypothetical protein -
MUY33_RS24435 (MUY33_24435) 113072..113257 - 186 WP_016674194 hypothetical protein -
MUY33_RS24440 (MUY33_24440) 113261..113485 - 225 WP_058670649 TraR/DksA C4-type zinc finger protein -
MUY33_RS24445 (MUY33_24445) 113605..114144 - 540 WP_025759821 type IV conjugative transfer system lipoprotein TraV traV
MUY33_RS24450 (MUY33_24450) 114166..115560 - 1395 WP_058654354 F-type conjugal transfer pilus assembly protein TraB traB
MUY33_RS24455 (MUY33_24455) 115547..116290 - 744 WP_008324157 type-F conjugative transfer system secretin TraK traK
MUY33_RS24460 (MUY33_24460) 116277..116843 - 567 WP_008324158 type IV conjugative transfer system protein TraE traE
MUY33_RS24465 (MUY33_24465) 116858..117163 - 306 WP_008324160 type IV conjugative transfer system protein TraL traL
MUY33_RS24470 (MUY33_24470) 117314..117664 - 351 WP_008324162 type IV conjugative transfer system pilin TraA -
MUY33_RS24475 (MUY33_24475) 117722..117946 - 225 WP_008324165 TraY domain-containing protein -
MUY33_RS24480 (MUY33_24480) 118033..118722 - 690 WP_008324166 hypothetical protein -
MUY33_RS24485 (MUY33_24485) 118899..119867 + 969 WP_160863459 IS5 family transposase -
MUY33_RS24490 (MUY33_24490) 119977..120369 - 393 WP_008324167 conjugal transfer relaxosome DNA-binding protein TraM -
MUY33_RS24495 (MUY33_24495) 120801..121286 + 486 WP_001568108 transglycosylase SLT domain-containing protein virB1
MUY33_RS24500 (MUY33_24500) 121319..121648 - 330 WP_011977736 DUF5983 family protein -
MUY33_RS24505 (MUY33_24505) 121681..122502 - 822 WP_023316395 DUF932 domain-containing protein -
MUY33_RS24510 (MUY33_24510) 123329..123892 - 564 WP_021312835 methyltransferase -
MUY33_RS24515 (MUY33_24515) 123940..125295 - 1356 WP_040228268 DUF3560 domain-containing protein -
MUY33_RS24520 (MUY33_24520) 125347..125577 - 231 WP_009310031 hypothetical protein -
MUY33_RS24525 (MUY33_24525) 125669..125896 - 228 WP_040228270 hypothetical protein -

Host bacterium

ID   8960 GenBank   NZ_CP095171
Plasmid name   pK666_mcr-10 Incompatibility group   IncFII
Plasmid size   136140 bp Coordinate of oriT [Strand]   120680..120728 [+]
Host baterium   Enterobacter roggenkampii strain K666

Cargo genes

Drug resistance gene   blaTEM-1B, blaLAP-2, qnrS1, mcr-10
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrVA2