Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   108517
Name   oriT_pS39-4-b in_silico
Organism   Enterococcus faecalis strain S39-4
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP088202 (25458..25487 [+], 30 nt)
oriT length   30 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 30 nt

>oriT_pS39-4-b
AACGTAAGTTAGTGGGTATAAGTGCGCCCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   5692 GenBank   WP_000974115
Name   mobQ_LQ044_RS14630_pS39-4-b insolico UniProt ID   A0A7H0FKC2
Length   661 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 661 a.a.        Molecular weight: 77412.46 Da        Isoelectric Point: 9.3565

>WP_000974115.1 MULTISPECIES: MobQ family relaxase [Bacilli]
MAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDRQTL
WNKMELAEKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHIMLT
MREVDSEGNILNKRKRIPKLDENGNQIFNEKGQRVTVSIKTNDWDRKSLVSEIRKDWADKVNQYLKDRNI
DQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDVLSELDKLENQEKVLKQDQN
FTLKFEKTFSPLEKKELKNLSKELKIFVNDENIDKRISELKRWENSLIFNNKMEIQKQRLMLSKISSERD
MLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVVEKEESKK
IFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDS
VIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKHID
ELSPLAKHNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSG
NQLQKKRNLDKLIKQTKAKENQSLQRNIPLR

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(18-242)

Nicking enzyme C-terminal middle helical domain (NES_C_h)

(289-394)


  Protein structure


Source ID Structure
AlphaFold DB A0A7H0FKC2


T4CP

ID   6069 GenBank   WP_224993997
Name   t4cp2_LQ044_RS14585_pS39-4-b insolico UniProt ID   _
Length   551 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 551 a.a.        Molecular weight: 63110.11 Da        Isoelectric Point: 4.7040

>WP_224993997.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Enterococcus]
MTSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFRTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLNLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVSEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQDYISSQTEFAKETIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELLMSDILSSFDEGIEENTTNNDNSELPF

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(33-456)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 10821..22992

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ044_RS14530 (LQ044_14530) 6413..7093 + 681 WP_013646119 IS6-like element IS1216 family transposase -
LQ044_RS14535 (LQ044_14535) 7123..7227 - 105 WP_224994000 NAD(P)H-dependent oxidoreductase -
LQ044_RS14540 (LQ044_14540) 7405..8832 - 1428 WP_032495690 chloramphenicol/florfenicol efflux MFS transporter FexA -
LQ044_RS14545 (LQ044_14545) 9542..9670 + 129 Protein_8 JAB domain-containing protein -
LQ044_RS14550 (LQ044_14550) 9802..10482 + 681 WP_013646119 IS6-like element IS1216 family transposase -
LQ044_RS14555 (LQ044_14555) 10536..10718 + 183 Protein_10 IS6 family transposase -
LQ044_RS14560 (LQ044_14560) 10821..11516 - 696 WP_249469437 LPXTG cell wall anchor domain-containing protein prgC
LQ044_RS14565 (LQ044_14565) 11576..11944 - 369 WP_000516429 hypothetical protein -
LQ044_RS14570 (LQ044_14570) 11973..12944 - 972 WP_002425009 hypothetical protein -
LQ044_RS14575 (LQ044_14575) 12961..13905 - 945 WP_194187774 conjugal transfer protein TrbL family protein trsL
LQ044_RS14580 (LQ044_14580) 13905..14828 - 924 WP_224993998 hypothetical protein traP
LQ044_RS14585 (LQ044_14585) 14846..16501 - 1656 WP_224993997 VirD4-like conjugal transfer protein, CD1115 family -
LQ044_RS14590 (LQ044_14590) 16494..16925 - 432 WP_224993996 hypothetical protein -
LQ044_RS14595 (LQ044_14595) 16930..17481 - 552 WP_224993995 hypothetical protein -
LQ044_RS14600 (LQ044_14600) 17494..18603 - 1110 WP_002425005 lysozyme family protein orf14
LQ044_RS14605 (LQ044_14605) 18625..19977 - 1353 WP_249469433 conjugal transfer protein TraF trsF
LQ044_RS14610 (LQ044_14610) 19991..21952 - 1962 WP_000411529 hypothetical protein virb4
LQ044_RS14615 (LQ044_14615) 21963..22592 - 630 WP_033626030 hypothetical protein -
LQ044_RS14620 (LQ044_14620) 22609..22992 - 384 WP_001083514 glycosyl transferase trsC
LQ044_RS14625 (LQ044_14625) 23011..23343 - 333 WP_000713503 CagC family type IV secretion system protein -
LQ044_RS14630 (LQ044_14630) 23367..25352 - 1986 WP_000974115 MobQ family relaxase -
LQ044_RS14635 (LQ044_14635) 25644..25943 + 300 WP_000431202 hypothetical protein -
LQ044_RS14640 (LQ044_14640) 25946..26203 + 258 WP_000002668 hypothetical protein -
LQ044_RS14645 (LQ044_14645) 26226..26531 - 306 WP_000351790 hypothetical protein -
LQ044_RS14650 (LQ044_14650) 26565..27062 - 498 WP_249469435 DnaJ domain-containing protein -
LQ044_RS14655 (LQ044_14655) 27101..27418 - 318 WP_002338433 hypothetical protein -

Host bacterium

ID   8952 GenBank   NZ_CP088202
Plasmid name   pS39-4-b Incompatibility group   -
Plasmid size   33618 bp Coordinate of oriT [Strand]   25458..25487 [+]
Host baterium   Enterococcus faecalis strain S39-4

Cargo genes

Drug resistance gene   Cfr(D), poxtA, fexA
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -