Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 108491
• Name: oriT_pS2-7-a
• Organism: Enterococcus hirae strain S2-7
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP088184 (7832..7871 [-], 40 nt)
• oriT length: 40 nt
• IRs (inverted repeats): 2..9, 11..18 (ACCTTATA..TATAAGGT)
• Location of nic site: 26..27
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 40 nt

>oriT_pS2-7-a
CACCTTATATTATAAGGTATAGTGTGTTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_LQ051_RS12830_pS2-7-a

• ID: 5678
• GenBank: WP_249464186
• Name: mobV_LQ051_RS12830_pS2-7-a
• UniProt ID: _
• Length: 407 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 407 a.a.
• Molecular weight: 47628.79 Da
• Isoelectric Point: 9.1467

>WP_249464186.1 MULTISPECIES: MobV family relaxase [Enterococcus]
MSMIVARMQKMKAENLIGIGNHNQRKTKNHSNPDIDTSLSELNYDLVNRTDNYKKDIENFINENKSTTRA
VRKDAVLINEWIITSDRKFFENLSDEDTKKFFITAKEYFEENFGEENIRYATVHLDESTPHLHMGIVPFD
KDKKLSAKRVFNREALRNVQEDLPKLLQEKGFDIERGLEGSDRKNLTVPEFKKLKEAEKEIERAIDRKKN
ELKAYTQENNISNKIDVNITRETKDVKVPSGEKFLGHEFTKTVQKTTGNLIISETDFKKLDQALKHGRIK
EEQLNKILNTDIHKENKELKDTVKELKAKTNDVIDDYNSLVKDYNSLIDENKGLRATISDLREEIRLIYI
STKEFLKDRTSDLQTFKSAFKDLIHTITNKIGNSSLENNFQKEYRKETRQQSRGMER

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

No available structure.

Host bacterium

• ID: 8926
• GenBank: NZ_CP088184
• Plasmid name: pS2-7-a
• Incompatibility group: -
• Plasmid size: 8617 bp
• Coordinate of oriT [Strand]: 7832..7871 [-]
• Host baterium: Enterococcus hirae strain S2-7

Cargo genes

• Drug resistance gene: optrA
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -