Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 108483 |
| Name | oriT_PR02395-7|unnamed_3  |
| Organism | Enterococcus faecium strain PR02395-7 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP064446 (64867..65058 [+], 192 nt) |
| oriT length | 192 nt |
| IRs (inverted repeats) | 106..112, 122..128 (ATTTTTT..AAAAAAT) 107..113, 120..126 (TTTTTTG..CAAAAAA) 93..98, 105..110 (AAAATA..TATTTT) 65..71, 75..81 (AGTTGGC..GCCAACT) 41..49, 52..60 (CACCTTCCT..AGGAAGGTG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 192 nt
>oriT_PR02395-7|unnamed_3
CCAAGGAATTAATACAAAGAGCATAAGGGAAAACTGATAGCACCTTCCTAAAGGAAGGTGGCTAAGTTGGCTGTGCCAACTGGTTTTCTTTCAAAATAGCTTCATATTTTTTGCTATCACAAAAAAATCCATTTTCGACCTATTTTCGGTCATAATATAGTACCTACTTTTGGTCATAGTTTCGTCCGTAGT
CCAAGGAATTAATACAAAGAGCATAAGGGAAAACTGATAGCACCTTCCTAAAGGAAGGTGGCTAAGTTGGCTGTGCCAACTGGTTTTCTTTCAAAATAGCTTCATATTTTTTGCTATCACAAAAAAATCCATTTTCGACCTATTTTCGGTCATAATATAGTACCTACTTTTGGTCATAGTTTCGTCCGTAGT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 5673 | GenBank | WP_002326870 |
| Name | mobP2_IUH70_RS15535_PR02395-7|unnamed_3 |
UniProt ID | B5U8V6 |
| Length | 506 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 506 a.a. Molecular weight: 60597.01 Da Isoelectric Point: 9.3485
>WP_002326870.1 MULTISPECIES: MobP2 family relaxase [Enterococcus]
MTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDYISRQEAIRQDKLDHDYSEDELNELARIEKAL
EKIEQETDQPVIKDLREMDKYIDYMTRKKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNG
SVMFQDVISFDNDFLVREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHV
TAMERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLELKQSVKDSLI
QLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTKEIDEL
YQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFDREKPEF
KTKYAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMI
EQEKMRQAREFEIGMN
MTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDYISRQEAIRQDKLDHDYSEDELNELARIEKAL
EKIEQETDQPVIKDLREMDKYIDYMTRKKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNG
SVMFQDVISFDNDFLVREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHV
TAMERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLELKQSVKDSLI
QLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTKEIDEL
YQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFDREKPEF
KTKYAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMI
EQEKMRQAREFEIGMN
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | B5U8V6 |
T4CP
| ID | 6052 | GenBank | WP_002311855 |
| Name | t4cp2_IUH70_RS15240_PR02395-7|unnamed_3 |
UniProt ID | _ |
| Length | 952 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 952 a.a. Molecular weight: 108225.12 Da Isoelectric Point: 7.4751
>WP_002311855.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Enterococcus]
MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIEGEDLPKSLQDKLNVPLVFIGVFLGSI
VFLAITYLLNFLKALLVAIGQSESGLLGVEFDPKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNH
ESEKNLVYGQKGDSRLSTIKEIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGK
GETFVVPLLDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVIDSWA
NKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDCIEKITMYNVAQMLNEL
GSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQGIEMFTSDLFGKLTSK
MSIDLKEIGFPKSIQFKVNTNLVGKRISISFLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLD
IRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNH
VLASIFISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVIQSYSQL
ETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVE
RASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDIACEHINLSL
QDNQIPYARFIKDLKTRLEYSIINNIPISEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLS
EQEMKINIYLKESQKEAIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQ
IMILEQQLANAYELAVFKTKNSILKNKIIKLKNAMTKLAKAI
MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIEGEDLPKSLQDKLNVPLVFIGVFLGSI
VFLAITYLLNFLKALLVAIGQSESGLLGVEFDPKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNH
ESEKNLVYGQKGDSRLSTIKEIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGK
GETFVVPLLDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVIDSWA
NKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDCIEKITMYNVAQMLNEL
GSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQGIEMFTSDLFGKLTSK
MSIDLKEIGFPKSIQFKVNTNLVGKRISISFLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLD
IRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNH
VLASIFISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVIQSYSQL
ETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVE
RASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDIACEHINLSL
QDNQIPYARFIKDLKTRLEYSIINNIPISEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLS
EQEMKINIYLKESQKEAIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQ
IMILEQQLANAYELAVFKTKNSILKNKIIKLKNAMTKLAKAI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 54..19715
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IUH70_RS15200 (IUH70_15180) | 54..1190 | - | 1137 | WP_002311861 | bifunctional lytic transglycosylase/C40 family peptidase | orf14 |
| IUH70_RS15205 (IUH70_15185) | 1212..1532 | - | 321 | WP_010729568 | hypothetical protein | virb4 |
| IUH70_RS15210 (IUH70_15190) | 1720..3597 | - | 1878 | WP_002305177 | group II intron reverse transcriptase/maturase | - |
| IUH70_RS15215 (IUH70_15195) | 4285..5910 | - | 1626 | WP_010729563 | hypothetical protein | virb4 |
| IUH70_RS15220 (IUH70_15200) | 5926..6393 | - | 468 | WP_002311859 | hypothetical protein | - |
| IUH70_RS15225 (IUH70_15205) | 6409..7026 | - | 618 | WP_002311858 | hypothetical protein | virb4 |
| IUH70_RS15230 (IUH70_15210) | 7023..7334 | - | 312 | WP_002317413 | DUF5592 family protein | - |
| IUH70_RS15235 (IUH70_15215) | 7382..10051 | - | 2670 | WP_002326868 | pLS20_p028 family conjugation system transmembrane protein | - |
| IUH70_RS15240 (IUH70_15220) | 10069..12927 | - | 2859 | WP_002311855 | VirD4-like conjugal transfer protein, CD1115 family | - |
| IUH70_RS15245 (IUH70_15225) | 12940..13656 | - | 717 | WP_002311854 | hypothetical protein | - |
| IUH70_RS15250 (IUH70_15230) | 13669..15495 | - | 1827 | WP_002311853 | hypothetical protein | - |
| IUH70_RS15255 (IUH70_15235) | 15604..15879 | - | 276 | WP_002311852 | TrbC/VirB2 family protein | virB2 |
| IUH70_RS15260 (IUH70_15240) | 15898..16119 | - | 222 | WP_002326867 | hypothetical protein | - |
| IUH70_RS15265 (IUH70_15250) | 16291..16653 | - | 363 | WP_002311851 | DUF4320 family protein | - |
| IUH70_RS15270 (IUH70_15255) | 16671..17468 | - | 798 | WP_002311850 | hypothetical protein | - |
| IUH70_RS15275 (IUH70_15260) | 17468..18283 | - | 816 | WP_002313315 | hypothetical protein | - |
| IUH70_RS15280 (IUH70_15265) | 18300..19715 | - | 1416 | WP_002311848 | CpaF/VirB11 family protein | virB11 |
| IUH70_RS15285 (IUH70_15270) | 19727..20149 | - | 423 | WP_002311847 | hypothetical protein | - |
| IUH70_RS15290 (IUH70_15275) | 20217..20369 | - | 153 | WP_002311846 | hypothetical protein | - |
| IUH70_RS15295 (IUH70_15280) | 20383..20745 | - | 363 | WP_002311845 | hypothetical protein | - |
| IUH70_RS15300 (IUH70_15285) | 20805..21590 | - | 786 | WP_046669026 | hypothetical protein | - |
| IUH70_RS15305 (IUH70_15290) | 21740..22966 | + | 1227 | WP_046650365 | IS21 family transposase | - |
| IUH70_RS15310 (IUH70_15295) | 22981..23736 | + | 756 | WP_010818485 | IS21-like element helper ATPase IstB | - |
Host bacterium
| ID | 8918 | GenBank | NZ_CP064446 |
| Plasmid name | PR02395-7|unnamed_3 | Incompatibility group | - |
| Plasmid size | 66916 bp | Coordinate of oriT [Strand] | 64867..65058 [+] |
| Host baterium | Enterococcus faecium strain PR02395-7 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA1 |