Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 108472
• Name: oriT_SESURV_p3_0825|unnamed3
• Organism: Staphylococcus epidermidis strain SESURV_p3_0825
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP043795 (2109..2182 [+], 74 nt)
• oriT length: 74 nt
• IRs (inverted repeats): 50..55, 62..67 (GGGGAT..ATCCCC) 26..32, 37..43 (TTTTTGT..ACAAAAA) 1..7, 16..22 (AGTGGCT..AGCCACT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 74 nt

>oriT_SESURV_p3_0825|unnamed3
AGTGGCTAGCATTTAAGCCACTCATTTTTTGTGTAAACAAAAACATAAGGGGGATGGGGATATCCCCATCAAGC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobL_F1622_RS11815_SESURV_p3_0825|unnamed3

• ID: 5667
• GenBank: WP_002456271
• Name: mobL_F1622_RS11815_SESURV_p3_0825|unnamed3
• UniProt ID: _
• Length: 273 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 273 a.a.
• Molecular weight: 32676.63 Da
• Isoelectric Point: 10.2967

>WP_002456271.1 MULTISPECIES: relaxase MobL [Bacillota]
MSEKTSPAINLICDFKVGSSKGESYKKFINYISTEKNKENVKKQDIEGNLFDKYLDYMSIDKLKKKQGYN
KENVKKRTTMSFNSEHEFIEEQEMNRLKDKFQEANKKSHVMYQDVVSFDNQFLKDNHLFNESLDKLDEPK
IKKATRRMVNQMIKDNKMDENHTFWCANIHYDTDNIHIHIATSEEKNTRKTIKEGRFKGQYRGKRTPTTL
RNMKSTFANSLYKDINLMKEIHQLKKEMRKEVKINLSRECKDWTASLNIKMKKLKKEMKRIRK

Protein domains

Predicted by InterproScan.

• MobL relaxases (MobL) (7-266)

Protein structure

No available structure.

Host bacterium

• ID: 8907
• GenBank: NZ_CP043795
• Plasmid name: SESURV_p3_0825|unnamed3
• Incompatibility group: -
• Plasmid size: 54389 bp
• Coordinate of oriT [Strand]: 2109..2182 [+]
• Host baterium: Staphylococcus epidermidis strain SESURV_p3_0825

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21