Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 108409 |
| Name | oriT_pAT48-b  |
| Organism | Enterococcus faecalis strain AT48 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP097012 (52788..52837 [-], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pAT48-b
AATATCGCAACATGGTACCATGTTGCTCTGCTCGCAAAAAGAAAGCCTAC
AATATCGCAACATGGTACCATGTTGCTCTGCTCGCAAAAAGAAAGCCTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 5620 | GenBank | WP_002405219 |
| Name | Relaxase_M2921_RS13955_pAT48-b |
UniProt ID | A0A3N3PAV0 |
| Length | 561 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 561 a.a. Molecular weight: 65340.10 Da Isoelectric Point: 6.1233
>WP_002405219.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Bacilli]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKVAAKEGAKIIEKSPKNS
HKKYTMWETESLYKEKIKSRLDFLLEQSSSINDFLEKAEALNLSVDFSRKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIVERLKENKSVLTLKEVVEKYVEKSEKAKNEFDYQLTIDEWQISHKTTRGYYVNVDFG
FGERGKVFIGAYKVDPLENGQYNIYVKRKDFFYLMNEKNAGRNRYMTGETLIKQLRLYNGQTPLKKEPVM
RTIDELVNAINFLAANEIEDTRQLELLEEKLEAAFLEAEETLETLDEKMLELHQLSNLLLENELNGELEL
IQQKLKTLLPDATLAEFSYEDVRGEIEAIKTSQSLLENKLERTRNEINQLHEIQAVQKKETANQNNVKPK
L
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKVAAKEGAKIIEKSPKNS
HKKYTMWETESLYKEKIKSRLDFLLEQSSSINDFLEKAEALNLSVDFSRKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIVERLKENKSVLTLKEVVEKYVEKSEKAKNEFDYQLTIDEWQISHKTTRGYYVNVDFG
FGERGKVFIGAYKVDPLENGQYNIYVKRKDFFYLMNEKNAGRNRYMTGETLIKQLRLYNGQTPLKKEPVM
RTIDELVNAINFLAANEIEDTRQLELLEEKLEAAFLEAEETLETLDEKMLELHQLSNLLLENELNGELEL
IQQKLKTLLPDATLAEFSYEDVRGEIEAIKTSQSLLENKLERTRNEINQLHEIQAVQKKETANQNNVKPK
L
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3N3PAV0 |
Auxiliary protein
| ID | 2706 | GenBank | WP_002367696 |
| Name | WP_002367696_pAT48-b |
UniProt ID | Q82YL4 |
| Length | 118 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 118 a.a. Molecular weight: 14042.10 Da Isoelectric Point: 9.9724
>WP_002367696.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Bacteria]
MENKQKLKRPIQRKIRFSKEENELINRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKEQS
MENKQKLKRPIQRKIRFSKEENELINRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKEQS
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q82YL4 |
T4CP
| ID | 6008 | GenBank | WP_010709219 |
| Name | t4cp2_M2921_RS13670_pAT48-b |
UniProt ID | _ |
| Length | 609 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 609 a.a. Molecular weight: 70047.09 Da Isoelectric Point: 6.0617
>WP_010709219.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEGEKTTGEDFWIKAEGLLVRSLIAYLWFDGRRNEYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYPKGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWCRYMTDEEAILDKISATDIIDHGEITSELKDETHV
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEGEKTTGEDFWIKAEGLLVRSLIAYLWFDGRRNEYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYPKGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWCRYMTDEEAILDKISATDIIDHGEITSELKDETHV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 150..17058
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| M2921_RS13675 (M2921_13680) | 150..635 | - | 486 | WP_002386493 | PcfB family protein | gbs1365 |
| M2921_RS13680 (M2921_13685) | 677..859 | - | 183 | WP_002367689 | hypothetical protein | - |
| M2921_RS13685 (M2921_13690) | 905..1363 | - | 459 | WP_002370354 | single-stranded DNA-binding protein | - |
| M2921_RS13690 (M2921_13695) | 1380..2135 | - | 756 | WP_002367687 | Fic family protein | - |
| M2921_RS13695 (M2921_13700) | 2248..2856 | - | 609 | WP_010709218 | hypothetical protein | - |
| M2921_RS13700 (M2921_13705) | 2840..3070 | - | 231 | WP_227660684 | type III secretion system protein PrgM | - |
| M2921_RS13705 (M2921_13710) | 3072..3698 | - | 627 | WP_002409284 | hypothetical protein | prgL |
| M2921_RS13710 (M2921_13715) | 3722..6337 | - | 2616 | WP_010709217 | phage tail tip lysozyme | prgK |
| M2921_RS13715 (M2921_13720) | 6349..8739 | - | 2391 | WP_002365863 | VirB4-like conjugal transfer ATPase, CD1110 family | virb4 |
| M2921_RS13720 (M2921_13725) | 8693..9046 | - | 354 | WP_002365862 | PrgI family mobile element protein | prgIc |
| M2921_RS13725 (M2921_13730) | 9048..9848 | - | 801 | WP_002367679 | type IV secretion system protein | prgHb |
| M2921_RS13730 (M2921_13735) | 9863..10159 | - | 297 | WP_002367677 | hypothetical protein | - |
| M2921_RS13735 (M2921_13740) | 10172..10399 | - | 228 | WP_002365858 | hypothetical protein | - |
| M2921_RS13740 (M2921_13745) | 10446..10880 | - | 435 | WP_002365857 | hypothetical protein | - |
| M2921_RS13745 (M2921_13750) | 10900..11799 | - | 900 | WP_002409287 | hypothetical protein | - |
| M2921_RS13750 (M2921_13755) | 11841..12698 | - | 858 | WP_010709216 | pheromone response LPXTG-anchored adhesin PrgC | prgC |
| M2921_RS13755 (M2921_13760) | 12726..13079 | - | 354 | WP_002367913 | pheromone response system RNA-binding regulator PrgU | - |
| M2921_RS13760 (M2921_13765) | 13141..17058 | - | 3918 | WP_202231790 | LPXTG-anchored aggregation substance | prgB |
| M2921_RS13765 (M2921_13770) | 17241..17474 | - | 234 | WP_229257464 | hypothetical protein | - |
| M2921_RS13770 (M2921_13775) | 17513..20191 | - | 2679 | WP_202231789 | surface exclusion protein SEA1/PrgA | - |
| M2921_RS13775 (M2921_13780) | 20202..20507 | - | 306 | WP_115252313 | hypothetical protein | - |
| M2921_RS13780 (M2921_13785) | 20809..21030 | - | 222 | WP_016616022 | hypothetical protein | - |
| M2921_RS13785 (M2921_13790) | 21081..21461 | - | 381 | WP_002365885 | PrgR | - |
Host bacterium
| ID | 8844 | GenBank | NZ_CP097012 |
| Plasmid name | pAT48-b | Incompatibility group | - |
| Plasmid size | 57161 bp | Coordinate of oriT [Strand] | 52788..52837 [-] |
| Host baterium | Enterococcus faecalis strain AT48 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |