Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 108406 |
| Name | oriT_p2-mcr  |
| Organism | Salmonella enterica strain |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP091575 (14174..14226 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_p2-mcr
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 6007 | GenBank | WP_065304450 |
| Name | t4cp2_INN74_RS26360_p2-mcr |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73526.12 Da Isoelectric Point: 9.2491
>WP_065304450.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNPVEIPLLFKDKTELMNKIARDAEIYLSDQKKVM
VAGGNVITNPDLDNHDKTDVSE
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNPVEIPLLFKDKTELMNKIARDAEIYLSDQKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 36243..60381
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| INN74_RS26300 (INN74_26300) | 31316..32542 | - | 1227 | WP_001672025 | RNA-guided endonuclease TnpB family protein | - |
| INN74_RS26305 (INN74_26305) | 32601..33005 | + | 405 | WP_001175008 | IS200/IS605 family transposase | - |
| INN74_RS26310 (INN74_26310) | 34305..35591 | - | 1287 | WP_015057162 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| INN74_RS26315 (INN74_26315) | 35604..36239 | - | 636 | WP_000934979 | A24 family peptidase | - |
| INN74_RS26320 (INN74_26320) | 36243..36725 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| INN74_RS26325 (INN74_26325) | 36791..37348 | - | 558 | WP_000095048 | type 4 pilus major pilin | - |
| INN74_RS26330 (INN74_26330) | 37393..38502 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| INN74_RS26335 (INN74_26335) | 38493..40031 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| INN74_RS26340 (INN74_26340) | 40056..40550 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| INN74_RS26345 (INN74_26345) | 40534..41844 | - | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| INN74_RS26350 (INN74_26350) | 41895..43538 | - | 1644 | WP_001035587 | PilN family type IVB pilus formation outer membrane protein | - |
| INN74_RS26355 (INN74_26355) | 43531..44061 | - | 531 | WP_001220542 | sigma 54-interacting transcriptional regulator | virb4 |
| INN74_RS26360 (INN74_26360) | 44108..46066 | - | 1959 | WP_065304450 | type IV secretory system conjugative DNA transfer family protein | - |
| INN74_RS26365 (INN74_26365) | 46082..47137 | - | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| INN74_RS26370 (INN74_26370) | 47156..48295 | - | 1140 | WP_054173905 | TrbI/VirB10 family protein | virB10 |
| INN74_RS26375 (INN74_26375) | 48285..48986 | - | 702 | WP_049824867 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| INN74_RS26380 (INN74_26380) | 49052..49786 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| INN74_RS26385 (INN74_26385) | 49786..49920 | - | 135 | WP_000701233 | hypothetical protein | - |
| INN74_RS26390 (INN74_26390) | 49952..52309 | - | 2358 | WP_065304451 | VirB4 family type IV secretion system protein | virb4 |
| INN74_RS26395 (INN74_26395) | 52315..52635 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| INN74_RS26400 (INN74_26400) | 52706..52996 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| INN74_RS26405 (INN74_26405) | 52996..53580 | - | 585 | WP_001177114 | lytic transglycosylase domain-containing protein | virB1 |
| INN74_RS26410 (INN74_26410) | 53601..53999 | - | 399 | WP_001153665 | hypothetical protein | - |
| INN74_RS26415 (INN74_26415) | 54118..54555 | - | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| INN74_RS26420 (INN74_26420) | 54561..55796 | - | 1236 | WP_015059538 | TcpQ domain-containing protein | - |
| INN74_RS26425 (INN74_26425) | 55799..56098 | - | 300 | WP_000835764 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| INN74_RS26430 (INN74_26430) | 56166..56447 | - | 282 | WP_000638823 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| INN74_RS26435 (INN74_26435) | 56437..56688 | - | 252 | WP_000121741 | hypothetical protein | - |
| INN74_RS26440 (INN74_26440) | 56788..57423 | - | 636 | WP_015059536 | hypothetical protein | - |
| INN74_RS26445 (INN74_26445) | 57567..58280 | + | 714 | WP_065304452 | DUF5710 domain-containing protein | - |
| INN74_RS26450 (INN74_26450) | 58503..58727 | - | 225 | WP_065304445 | EexN family lipoprotein | - |
| INN74_RS26455 (INN74_26455) | 58736..59380 | - | 645 | WP_065304453 | type IV secretion system protein | - |
| INN74_RS26460 (INN74_26460) | 59386..60381 | - | 996 | WP_065304446 | type IV secretion system protein | virB6 |
| INN74_RS26465 (INN74_26465) | 60385..60642 | - | 258 | WP_000739144 | hypothetical protein | - |
| INN74_RS26470 (INN74_26470) | 60639..60941 | - | 303 | WP_065304447 | hypothetical protein | - |
| INN74_RS26475 (INN74_26475) | 61156..61464 | - | 309 | WP_236492988 | hypothetical protein | - |
| INN74_RS26480 (INN74_26480) | 61511..61681 | - | 171 | WP_044069355 | hypothetical protein | - |
| INN74_RS26485 (INN74_26485) | 61685..62128 | - | 444 | WP_071961414 | NfeD family protein | - |
| INN74_RS26490 (INN74_26490) | 62502..63455 | - | 954 | WP_072089442 | SPFH domain-containing protein | - |
| INN74_RS26495 (INN74_26495) | 63501..63695 | - | 195 | WP_001127356 | DUF1187 family protein | - |
| INN74_RS26500 (INN74_26500) | 63688..64140 | - | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 8841 | GenBank | NZ_CP091575 |
| Plasmid name | p2-mcr | Incompatibility group | IncI2 |
| Plasmid size | 65271 bp | Coordinate of oriT [Strand] | 14174..14226 [-] |
| Host baterium | Salmonella enterica strain |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |