Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   108404
Name   oriT_p2-3 in_silico
Organism   Salmonella enterica strain
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP091573 (31005..31093 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_p2-3
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   5618 GenBank   WP_012783930
Name   nikB_INN74_RS25380_p2-3 insolico UniProt ID   N0GUP8
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104070.55 Da        Isoelectric Point: 7.3623

>WP_012783930.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSHMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLAERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWLPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALVDKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB N0GUP8


Auxiliary protein

ID   2703 GenBank   WP_001283947
Name   WP_001283947_p2-3 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   6004 GenBank   WP_012783931
Name   trbC_INN74_RS25385_p2-3 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86911.02 Da        Isoelectric Point: 6.7713

>WP_012783931.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILTAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 36471..76202

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
INN74_RS25385 (INN74_25385) 34187..36478 - 2292 WP_012783931 F-type conjugative transfer protein TrbC -
INN74_RS25390 (INN74_25390) 36471..37541 - 1071 WP_012783932 IncI1-type conjugal transfer protein TrbB trbB
INN74_RS25395 (INN74_25395) 37560..38768 - 1209 WP_001703845 IncI1-type conjugal transfer protein TrbA trbA
INN74_RS25400 (INN74_25400) 39075..39854 - 780 WP_275450201 protein FinQ -
INN74_RS25405 (INN74_25405) 40481..40633 + 153 WP_001387489 Hok/Gef family protein -
INN74_RS25410 (INN74_25410) 40705..40956 - 252 WP_001291964 hypothetical protein -
INN74_RS26575 41457..41552 + 96 WP_000609148 DinQ-like type I toxin DqlB -
INN74_RS25420 (INN74_25420) 41617..41793 - 177 WP_001054900 hypothetical protein -
INN74_RS25425 (INN74_25425) 42185..42394 + 210 WP_000062603 HEAT repeat domain-containing protein -
INN74_RS25430 (INN74_25430) 42466..43128 - 663 WP_012783935 plasmid IncI1-type surface exclusion protein ExcA -
INN74_RS25435 (INN74_25435) 43199..45367 - 2169 WP_015508354 DotA/TraY family protein traY
INN74_RS25440 (INN74_25440) 45464..46048 - 585 WP_012783937 conjugal transfer protein TraX -
INN74_RS25445 (INN74_25445) 46077..47279 - 1203 WP_012783938 IncI1-type conjugal transfer protein TraW traW
INN74_RS25450 (INN74_25450) 47246..47860 - 615 WP_000337394 IncI1-type conjugal transfer protein TraV traV
INN74_RS25455 (INN74_25455) 47860..50904 - 3045 WP_012783939 IncI1-type conjugal transfer protein TraU traU
INN74_RS25460 (INN74_25460) 50994..51794 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
INN74_RS25465 (INN74_25465) 51778..51966 - 189 WP_001277255 putative conjugal transfer protein TraS -
INN74_RS25470 (INN74_25470) 52030..52434 - 405 WP_000086965 IncI1-type conjugal transfer protein TraR traR
INN74_RS25475 (INN74_25475) 52485..53012 - 528 WP_001055569 conjugal transfer protein TraQ traQ
INN74_RS25480 (INN74_25480) 53012..53716 - 705 WP_001493811 IncI1-type conjugal transfer protein TraP traP
INN74_RS25485 (INN74_25485) 53716..55005 - 1290 WP_011264042 conjugal transfer protein TraO traO
INN74_RS25490 (INN74_25490) 55008..55991 - 984 WP_001191880 IncI1-type conjugal transfer protein TraN traN
INN74_RS25495 (INN74_25495) 56002..56694 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
INN74_RS25500 (INN74_25500) 56691..57038 - 348 WP_001055900 conjugal transfer protein traL
INN74_RS25505 (INN74_25505) 57056..60823 - 3768 WP_012783940 LPD7 domain-containing protein -
INN74_RS25510 (INN74_25510) 60913..61464 - 552 WP_012783942 phospholipase D family protein -
INN74_RS25515 (INN74_25515) 61479..61769 - 291 WP_001314267 hypothetical protein traK
INN74_RS25520 (INN74_25520) 61766..62914 - 1149 WP_001024971 plasmid transfer ATPase TraJ virB11
INN74_RS25525 (INN74_25525) 62911..63729 - 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
INN74_RS25530 (INN74_25530) 63726..64184 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
INN74_RS25535 (INN74_25535) 64578..65162 - 585 WP_000977522 histidine phosphatase family protein -
INN74_RS25540 (INN74_25540) 65222..66424 - 1203 WP_000976353 conjugal transfer protein TraF -
INN74_RS25545 (INN74_25545) 66509..67333 - 825 WP_001238939 conjugal transfer protein TraE traE
INN74_RS25550 (INN74_25550) 67484..68638 - 1155 WP_001139957 site-specific integrase -
INN74_RS26660 70054..70275 + 222 Protein_78 prepilin -
INN74_RS25555 (INN74_25555) 70272..71696 - 1425 WP_001389385 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
INN74_RS25560 (INN74_25560) 71696..72352 - 657 WP_001193553 prepilin peptidase -
INN74_RS25565 (INN74_25565) 72337..72897 - 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
INN74_RS25570 (INN74_25570) 72907..73521 - 615 WP_000959785 type 4 pilus major pilin -
INN74_RS25575 (INN74_25575) 73539..74636 - 1098 WP_001208805 type II secretion system F family protein -
INN74_RS25580 (INN74_25580) 74649..76202 - 1554 WP_000362202 ATPase, T2SS/T4P/T4SS family virB11
INN74_RS25585 (INN74_25585) 76213..76665 - 453 WP_001247336 type IV pilus biogenesis protein PilP -
INN74_RS25590 (INN74_25590) 76652..77947 - 1296 WP_000752774 type 4b pilus protein PilO2 -
INN74_RS25595 (INN74_25595) 77940..79622 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
INN74_RS25600 (INN74_25600) 79636..80073 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
INN74_RS25605 (INN74_25605) 80073..81140 - 1068 WP_000742600 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   8839 GenBank   NZ_CP091573
Plasmid name   p2-3 Incompatibility group   IncI1
Plasmid size   86718 bp Coordinate of oriT [Strand]   31005..31093 [-]
Host baterium   Salmonella enterica strain

Cargo genes

Drug resistance gene   blaCTX-M-55
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrVA2