Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 108397
• Name: oriT_pAT03-a
• Organism: Vagococcus lutrae strain AT03
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP097073 (539..578 [-], 40 nt)
• oriT length: 40 nt
• IRs (inverted repeats): 13..21, 28..36 (TAAAGTATA..TATACTTTA) 2..8, 12..18 (ACTTTAT..ATAAAGT)
• Location of nic site: 26..27
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 40 nt

>oriT_pAT03-a
CACTTTATATCATAAAGTATAGTGTGTTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_M2901_RS10025_pAT03-a

• ID: 5612
• GenBank: WP_248856309
• Name: mobV_M2901_RS10025_pAT03-a
• UniProt ID: _
• Length: 443 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 443 a.a.
• Molecular weight: 51976.82 Da
• Isoelectric Point: 9.4462

>WP_248856309.1 MobV family relaxase [Vagococcus lutrae]
MSDSVSMIVARMQKMKAGNLTGSQKHNQREFKNHSNEDIDQDREHLNYDLVNDEPINYQEKIKEIIESQR
ESTRAIRKDAVLVNEWIITSNQLFFKDMNQENLNLFFETALDYFSSKFGSQNIAYAQVHLDETTPHMHLG
IVPMSDGKLSSKTVFNRQTLREIQDELPKILKENGFEIERGNENSERKNLSVATYKEVVNEAKKKAELEA
QEITKELKNVTKERQIELKKLNTSKQQLEEKNKQLQGKVADIYNIYKLDYGFDQFERNLERTMFGKRIIT
ETELRKLQKTFNGLRSKAIELKDKSDKQARINDQLRDKLKKVERDFKVASDRRQELNQENNTLSRQVATL
KEKNTVYRDILKHDYGLNGISQREFDARLVLNEIESGYKPPNRRTGELWVETLENATDTKIEPSRLARGL
ELAKAMLQKLIELARKRDRDMER

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (7-197)

Protein structure

No available structure.

Host bacterium

• ID: 8832
• GenBank: NZ_CP097073
• Plasmid name: pAT03-a
• Incompatibility group: -
• Plasmid size: 10004 bp
• Coordinate of oriT [Strand]: 539..578 [-]
• Host baterium: Vagococcus lutrae strain AT03

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -