Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 108396 |
| Name | oriT_pAT40b-a  |
| Organism | Enterococcus faecalis strain AT40b |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP097035 (31833..31870 [+], 38 nt) |
| oriT length | 38 nt |
| IRs (inverted repeats) | 7..12, 16..21 (AGTAAC..GTTACT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 38 nt
>oriT_pAT40b-a
ATACGAAGTAACGAAGTTACTGCGTATAAGTGCGCCCT
ATACGAAGTAACGAAGTTACTGCGTATAAGTGCGCCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 5611 | GenBank | WP_248878838 |
| Name | MobA_MobL_M2913_RS13620_pAT40b-a |
UniProt ID | _ |
| Length | 664 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 664 a.a. Molecular weight: 77604.76 Da Isoelectric Point: 9.2665
>WP_248878838.1 MobA/MobL family protein [Enterococcus faecalis]
MIQLAIFHLSMTIAKRENGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDR
QTLWNKMELAEKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVNEGMIADVAIHRDDENNPHAHI
MLTMREVDSEGNILNKSHRIPKLDENGNQIFNEKGQRVTVSIKTNDWGRKSLVSEIRKDWADKVNQYLKD
RNIDQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDILAELDKLENQEKVLKQ
DQNFTLKFEKTFSPLEKGELKNLSKELKLFINDENIDKRLGELKRWENSLIFNNKMEIQKQRLMLSKISS
ERDMLTKANEILDKQAEIFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVVEKEE
SKKIFKEKPFQTSRYLDSKIKQIEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVS
IDSVIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMK
HIDELSPLAKQNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRL
LSGNQLQKKRNLDKLIKQTKAKKNQSLQRNIPLR
MIQLAIFHLSMTIAKRENGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDR
QTLWNKMELAEKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVNEGMIADVAIHRDDENNPHAHI
MLTMREVDSEGNILNKSHRIPKLDENGNQIFNEKGQRVTVSIKTNDWGRKSLVSEIRKDWADKVNQYLKD
RNIDQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDILAELDKLENQEKVLKQ
DQNFTLKFEKTFSPLEKGELKNLSKELKLFINDENIDKRLGELKRWENSLIFNNKMEIQKQRLMLSKISS
ERDMLTKANEILDKQAEIFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVVEKEE
SKKIFKEKPFQTSRYLDSKIKQIEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVS
IDSVIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMK
HIDELSPLAKQNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRL
LSGNQLQKKRNLDKLIKQTKAKKNQSLQRNIPLR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 5997 | GenBank | WP_248878830 |
| Name | t4cp2_M2913_RS13575_pAT40b-a |
UniProt ID | _ |
| Length | 551 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 551 a.a. Molecular weight: 63084.20 Da Isoelectric Point: 4.7420
>WP_248878830.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MTSLLAESDGLILGKFPSGKVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLSLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVSEFTSTNDFFFEELGTSKICLYVLISPLERTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQDYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELLMSDILSSFDEGIEENTANNDNSELPF
MTSLLAESDGLILGKFPSGKVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLSLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVSEFTSTNDFFFEELGTSKICLYVLISPLERTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQDYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELLMSDILSSFDEGIEENTANNDNSELPF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 19354..29375
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| M2913_RS13545 (M2913_13545) | 14471..15628 | - | 1158 | WP_248878824 | hypothetical protein | - |
| M2913_RS13550 (M2913_13550) | 16150..17322 | - | 1173 | WP_248878825 | IS256-like element IS1542 family transposase | - |
| M2913_RS13555 (M2913_13555) | 17987..18340 | - | 354 | WP_002301645 | hypothetical protein | - |
| M2913_RS13560 (M2913_13560) | 18369..19337 | - | 969 | WP_002377195 | hypothetical protein | - |
| M2913_RS13565 (M2913_13565) | 19354..20286 | - | 933 | WP_248878826 | conjugal transfer protein TrbL family protein | trsL |
| M2913_RS13570 (M2913_13570) | 20288..21211 | - | 924 | WP_248878828 | hypothetical protein | - |
| M2913_RS13575 (M2913_13575) | 21229..22884 | - | 1656 | WP_248878830 | VirD4-like conjugal transfer protein, CD1115 family | - |
| M2913_RS13580 (M2913_13580) | 22877..23308 | - | 432 | WP_108173621 | hypothetical protein | - |
| M2913_RS13585 (M2913_13585) | 23313..23864 | - | 552 | WP_248878832 | hypothetical protein | - |
| M2913_RS13590 (M2913_13590) | 23877..24986 | - | 1110 | WP_248878834 | lysozyme family protein | orf14 |
| M2913_RS13595 (M2913_13595) | 25008..26360 | - | 1353 | WP_125958265 | conjugal transfer protein TraF | trsF |
| M2913_RS13600 (M2913_13600) | 26374..28335 | - | 1962 | WP_248878836 | TrsE protein | virb4 |
| M2913_RS13605 (M2913_13605) | 28346..28975 | - | 630 | WP_002332650 | hypothetical protein | trsD |
| M2913_RS13610 (M2913_13610) | 28992..29375 | - | 384 | WP_002332651 | hypothetical protein | trsC |
| M2913_RS13615 (M2913_13615) | 29394..29726 | - | 333 | WP_000713503 | CagC family type IV secretion system protein | - |
| M2913_RS13620 (M2913_13620) | 29750..31735 | - | 1986 | WP_346429110 | MobQ family relaxase | - |
| M2913_RS13625 (M2913_13625) | 32027..32326 | + | 300 | WP_002332653 | hypothetical protein | - |
| M2913_RS13630 (M2913_13630) | 32329..32586 | + | 258 | WP_000002668 | hypothetical protein | - |
| M2913_RS13635 (M2913_13635) | 32680..32913 | - | 234 | WP_002332654 | hypothetical protein | - |
| M2913_RS13640 (M2913_13640) | 32947..33444 | - | 498 | WP_002338758 | DnaJ domain-containing protein | - |
| M2913_RS13645 (M2913_13645) | 33978..34295 | - | 318 | WP_002326830 | hypothetical protein | - |
Host bacterium
| ID | 8831 | GenBank | NZ_CP097035 |
| Plasmid name | pAT40b-a | Incompatibility group | - |
| Plasmid size | 92441 bp | Coordinate of oriT [Strand] | 31833..31870 [+] |
| Host baterium | Enterococcus faecalis strain AT40b |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | tcrB |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |