Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 108395
• Name: oriT1_pAT34-a
• Organism: Enterococcus faecalis strain AT34
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP097043 (7002..7041 [-], 40 nt)
• oriT length: 40 nt
• IRs (inverted repeats): 2..8, 12..18 (ACTTTAC..GTAAAGT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 40 nt

>oriT1_pAT34-a
CACTTTACGAAGTAAAGTATAGTGTATTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_M2910_RS13000_pAT34-a

• ID: 5610
• GenBank: WP_000119405
• Name: mobV_M2910_RS13000_pAT34-a
• UniProt ID: F5X340
• Length: 420 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 420 a.a.
• Molecular weight: 49660.33 Da
• Isoelectric Point: 9.9693

>WP_000119405.1 MULTISPECIES: MobV family relaxase [Bacteria]
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDN
DRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSL
IYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

• AlphaFold DB: F5X340

Host bacterium

• ID: 8830
• GenBank: NZ_CP097043
• Plasmid name: pAT34-a
• Incompatibility group: -
• Plasmid size: 40376 bp
• Coordinate of oriT [Strand]: 7002..7041 [-]; 5586..5611 [-]
• Host baterium: Enterococcus faecalis strain AT34

Cargo genes

• Drug resistance gene: cat, tet(L), tet(M), fexA, optrA, erm(B)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21