Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 108356 |
| Name | oriT_PA-2-9E|p1  |
| Organism | Pseudomonas syringae strain PA-2-9E |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP066266 (55280..55459 [+], 180 nt) |
| oriT length | 180 nt |
| IRs (inverted repeats) | 80..87, 91..98 (CAAAGGGG..CCCCTTTG) 42..47, 56..61 (AAAAAG..CTTTTT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 180 nt
>oriT_PA-2-9E|p1
ATCTCGAATTCGCTAAACAGGCACGTTACCGATTGTCTACAAAAAAGAGCTTCGCCTTTTTCGTATCCAATCGACGTGCCAAAGGGGATACCCCTTTGAAACCCCGCAGAGCGGGAAAGCGTTGTCGTTGTCGTTGTTGGTTGTTGTCATCCAGGTGCCCTCAGGAGGTGATTTTGTGGC
ATCTCGAATTCGCTAAACAGGCACGTTACCGATTGTCTACAAAAAAGAGCTTCGCCTTTTTCGTATCCAATCGACGTGCCAAAGGGGATACCCCTTTGAAACCCCGCAGAGCGGGAAAGCGTTGTCGTTGTCGTTGTTGGTTGTTGTCATCCAGGTGCCCTCAGGAGGTGATTTTGTGGC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 5588 | GenBank | WP_080034374 |
| Name | Relaxase_I9H07_RS24850_PA-2-9E|p1 |
UniProt ID | _ |
| Length | 1249 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1249 a.a. Molecular weight: 138620.90 Da Isoelectric Point: 9.1124
>WP_080034374.1 MULTISPECIES: LPD7 domain-containing protein [Pseudomonas syringae group]
MLIRVSGYNTGAQEYLEQGNKSGREFTRDELDHRLIIEGQLSVTRAIYESIPDHGQDRYLTFTLSFKEDT
VSPELLKAVTTEFKNFFMHAYKPEEFNLYAEAHLPKMKTVTNRKTGEVIDRKPHIHIIIPRINLLSGNEA
NPVDVYKNHEKYFEAFQEHINQKYGLSSPRENVRADITDAASVLSRYKGDDFYGKNRQFKQQLVKQVIER
GVNSRADFYALVAEHGETRIRNEGKDNEYISVKLPGDAKGTNLKDTIFQDDFIVRRELRKPPLEASVIQE
RLLAWPQRAREIKYVNKATPKFRKQYSQASPEERVRLLAERETNFYRAHGDDYESVHTWQRQRDNQRSPA
ETTGRRTAAPADGLQDLSVSDVADHRQAGPTRSRDGALLLPSDAHVHVGQSQPGGDSGLRSSVPGGGRGR
RAGSTAERGRGGSQPAAVSQETKGTATPAGATGRRRAGAGKSRNTRVRAGRVVPPYAQNPHRVATIADIE
ERGRRLFDPLKRPSDNALVFYRSSSHPQVADQLPQARAATTTPVQSTAGRRPRSSGKPRPPRQWRPGAVP
PYAKNPHRVATVADIEQRARMLFDPLKRPADKALVFKRASIKALTVNKHASTVAAYFTRQAQHNQIAPAH
RRAIRRIDQQYFALRRAVFSDQRLTRQDKAQLVSVLTFERLKAREQFHKPQPNIEVNLMGSAAIRNLLDD
EKEDPGFSISGARGPGREGVRDQVKRIMDRFAKQVDPDAATERARDLSAKDLYTRKAKFSQNVHYLDKQT
DKTLFVDTGTTISMRRTGITEAGVSVALQLARERFGSTLTINGTAEFKKLVIEAVAKNGLDVHFTDKAMN
QSLADRRAELDIERDGQSIGPATDLPRHVDDATRDVRDQADRLGVTVPIEALYGQGKTADQVSQALTAQL
DTVPESERIAFVETVAITLGIPERGQPKGDQAFAQWQAQRAQPAANSAASATSEAQANVPTPSDAVPEPV
IPEAAKPALVNDPDLQSPSELVRLEAQWRRDFPMSEADVRASDTVMGLRGEDHAIWIIATDDKTPEAAAL
LTAYMENDSYREAFKASIVAAYKQVENSPQSVDDLDHLTAMAAQIVNEVEERLFPSPQAATGQTAPSRSK
VIEGTLIDHGEAPYQHNDDNQMSYFVTLKPEGGKPRTVWGVGLEEAMSDADLKQGDQVRLRDLGTQPVVV
QVIEEDGTVTDKTVNRREWSAQPVAPEREVAETTPKGQAAAAGTPELSSPDEDDGMSVD
MLIRVSGYNTGAQEYLEQGNKSGREFTRDELDHRLIIEGQLSVTRAIYESIPDHGQDRYLTFTLSFKEDT
VSPELLKAVTTEFKNFFMHAYKPEEFNLYAEAHLPKMKTVTNRKTGEVIDRKPHIHIIIPRINLLSGNEA
NPVDVYKNHEKYFEAFQEHINQKYGLSSPRENVRADITDAASVLSRYKGDDFYGKNRQFKQQLVKQVIER
GVNSRADFYALVAEHGETRIRNEGKDNEYISVKLPGDAKGTNLKDTIFQDDFIVRRELRKPPLEASVIQE
RLLAWPQRAREIKYVNKATPKFRKQYSQASPEERVRLLAERETNFYRAHGDDYESVHTWQRQRDNQRSPA
ETTGRRTAAPADGLQDLSVSDVADHRQAGPTRSRDGALLLPSDAHVHVGQSQPGGDSGLRSSVPGGGRGR
RAGSTAERGRGGSQPAAVSQETKGTATPAGATGRRRAGAGKSRNTRVRAGRVVPPYAQNPHRVATIADIE
ERGRRLFDPLKRPSDNALVFYRSSSHPQVADQLPQARAATTTPVQSTAGRRPRSSGKPRPPRQWRPGAVP
PYAKNPHRVATVADIEQRARMLFDPLKRPADKALVFKRASIKALTVNKHASTVAAYFTRQAQHNQIAPAH
RRAIRRIDQQYFALRRAVFSDQRLTRQDKAQLVSVLTFERLKAREQFHKPQPNIEVNLMGSAAIRNLLDD
EKEDPGFSISGARGPGREGVRDQVKRIMDRFAKQVDPDAATERARDLSAKDLYTRKAKFSQNVHYLDKQT
DKTLFVDTGTTISMRRTGITEAGVSVALQLARERFGSTLTINGTAEFKKLVIEAVAKNGLDVHFTDKAMN
QSLADRRAELDIERDGQSIGPATDLPRHVDDATRDVRDQADRLGVTVPIEALYGQGKTADQVSQALTAQL
DTVPESERIAFVETVAITLGIPERGQPKGDQAFAQWQAQRAQPAANSAASATSEAQANVPTPSDAVPEPV
IPEAAKPALVNDPDLQSPSELVRLEAQWRRDFPMSEADVRASDTVMGLRGEDHAIWIIATDDKTPEAAAL
LTAYMENDSYREAFKASIVAAYKQVENSPQSVDDLDHLTAMAAQIVNEVEERLFPSPQAATGQTAPSRSK
VIEGTLIDHGEAPYQHNDDNQMSYFVTLKPEGGKPRTVWGVGLEEAMSDADLKQGDQVRLRDLGTQPVVV
QVIEEDGTVTDKTVNRREWSAQPVAPEREVAETTPKGQAAAAGTPELSSPDEDDGMSVD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 5967 | GenBank | WP_010217011 |
| Name | t4cp2_I9H07_RS24805_PA-2-9E|p1 |
UniProt ID | _ |
| Length | 550 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 550 a.a. Molecular weight: 61600.27 Da Isoelectric Point: 6.0604
>WP_010217011.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Pseudomonas syringae group]
MGKAKSKPISSTNPQERIEEYPAFLLGKHPTKDVFLASYGQQFVMLAAPPGSMKGVSAVIPNLLSYPDSM
VVNDPKFENWDITSGFRASAGHKVFRFSPERLETHRWNPVSAINRDPLYRLGDIRTLARVLFVSDNPKNQ
EWYNKAGNVFTSILLYLMETPEMPCTLPQAYEIGSLGTGIGTWAQQIIELRSIGPNALSFETLRELNGVY
EASKNKSSGWSTTVDIVRDVLSVYAEKTVAWAVSGDDIDFAKMREEKTTVYFSVTEGNLKKYGPLMNLFF
TQAIRLNSKVLPEQGGHCADGTLRYKYQLALMMDEFAIMGRMEIMETAPALTRGAGLRFFLIFQGKDQIR
AVYGEEAANGIMKAIHNEIVFAPGDIKLAEEYSRRLGNTTERVHNQSLNRQKNEIGARGQTDSYSEQARP
LLLPQEVNELPFDKQLIFVQGNRQTEPMKILARKIIYFEEDVFKARQKMTPPPLPVGDATKIDALTVPVR
TIEAKVAVADTKPMQAEQRQRWNPKEKAANAVPEQPDAAQPVDVEPDPEPAQADDTPETM
MGKAKSKPISSTNPQERIEEYPAFLLGKHPTKDVFLASYGQQFVMLAAPPGSMKGVSAVIPNLLSYPDSM
VVNDPKFENWDITSGFRASAGHKVFRFSPERLETHRWNPVSAINRDPLYRLGDIRTLARVLFVSDNPKNQ
EWYNKAGNVFTSILLYLMETPEMPCTLPQAYEIGSLGTGIGTWAQQIIELRSIGPNALSFETLRELNGVY
EASKNKSSGWSTTVDIVRDVLSVYAEKTVAWAVSGDDIDFAKMREEKTTVYFSVTEGNLKKYGPLMNLFF
TQAIRLNSKVLPEQGGHCADGTLRYKYQLALMMDEFAIMGRMEIMETAPALTRGAGLRFFLIFQGKDQIR
AVYGEEAANGIMKAIHNEIVFAPGDIKLAEEYSRRLGNTTERVHNQSLNRQKNEIGARGQTDSYSEQARP
LLLPQEVNELPFDKQLIFVQGNRQTEPMKILARKIIYFEEDVFKARQKMTPPPLPVGDATKIDALTVPVR
TIEAKVAVADTKPMQAEQRQRWNPKEKAANAVPEQPDAAQPVDVEPDPEPAQADDTPETM
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 36099..46296
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| I9H07_RS24695 (I9H07_24555) | 31173..32288 | - | 1116 | WP_005782495 | hypothetical protein | - |
| I9H07_RS24700 (I9H07_24560) | 32340..33113 | - | 774 | WP_005782492 | helix-turn-helix transcriptional regulator | - |
| I9H07_RS24705 (I9H07_24565) | 33222..34322 | + | 1101 | WP_010199864 | polyamine ABC transporter substrate-binding protein | - |
| I9H07_RS24710 (I9H07_24570) | 34421..34618 | + | 198 | WP_005782488 | hypothetical protein | - |
| I9H07_RS24715 (I9H07_24575) | 34630..35025 | - | 396 | WP_005782487 | helix-turn-helix transcriptional regulator | - |
| I9H07_RS24720 (I9H07_24580) | 35243..35755 | + | 513 | WP_010199861 | transcription termination/antitermination NusG family protein | - |
| I9H07_RS24725 (I9H07_24585) | 35844..36086 | + | 243 | WP_005782484 | hypothetical protein | - |
| I9H07_RS24730 (I9H07_24590) | 36099..36803 | + | 705 | WP_005782481 | lytic transglycosylase domain-containing protein | virB1 |
| I9H07_RS24735 (I9H07_24595) | 36803..37138 | + | 336 | WP_005782479 | hypothetical protein | - |
| I9H07_RS24740 (I9H07_24600) | 37151..37639 | + | 489 | WP_005782477 | VirB3 family type IV secretion system protein | virB3 |
| I9H07_RS24745 (I9H07_24605) | 37542..40040 | + | 2499 | WP_223886542 | conjugal transfer protein | virb4 |
| I9H07_RS24750 (I9H07_24610) | 40037..40717 | + | 681 | WP_005782474 | P-type DNA transfer protein VirB5 | - |
| I9H07_RS24755 (I9H07_24615) | 40733..40939 | + | 207 | WP_005782472 | hypothetical protein | - |
| I9H07_RS24760 (I9H07_24620) | 40966..41910 | + | 945 | WP_010199857 | type IV secretion system protein | virB6 |
| I9H07_RS24765 (I9H07_24625) | 41971..42273 | + | 303 | WP_080034376 | conjugal transfer protein | - |
| I9H07_RS24770 (I9H07_24630) | 42311..43099 | + | 789 | WP_010199854 | type IV secretion system protein | virB8 |
| I9H07_RS24775 (I9H07_24635) | 43089..43898 | + | 810 | WP_005782466 | P-type conjugative transfer protein VirB9 | virB9 |
| I9H07_RS24780 (I9H07_24640) | 43885..45255 | + | 1371 | WP_010199849 | TrbI/VirB10 family protein | virB10 |
| I9H07_RS24785 (I9H07_24645) | 45265..46296 | + | 1032 | WP_005782462 | P-type DNA transfer ATPase VirB11 | virB11 |
| I9H07_RS24790 (I9H07_24650) | 46306..46662 | + | 357 | WP_010217013 | hypothetical protein | - |
| I9H07_RS24795 (I9H07_24655) | 46782..47099 | + | 318 | WP_005782454 | hypothetical protein | - |
| I9H07_RS24800 (I9H07_24660) | 47109..47342 | + | 234 | WP_005782456 | hypothetical protein | - |
| I9H07_RS24805 (I9H07_24665) | 47342..48994 | + | 1653 | WP_010217011 | type IV secretory system conjugative DNA transfer family protein | - |
| I9H07_RS24810 (I9H07_24670) | 49035..49397 | + | 363 | WP_005782555 | TrbM/KikA/MpfK family conjugal transfer protein | - |
Host bacterium
| ID | 8791 | GenBank | NZ_CP066266 |
| Plasmid name | PA-2-9E|p1 | Incompatibility group | - |
| Plasmid size | 61608 bp | Coordinate of oriT [Strand] | 55280..55459 [+] |
| Host baterium | Pseudomonas syringae strain PA-2-9E |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | copS, copR, cueA, cusA/ybdE, copC, copA |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |