Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 108350
• Name: oriT_pMGB0941-2
• Organism: Pediococcus pentosaceus strain MGB0941
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP085180 (3430..3450 [-], 21 nt)
• oriT length: 21 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 21 nt

>oriT_pMGB0941-2
AGTGGGGTGTAAGTGCGCATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobQ_Ped0941_RS09555_pMGB0941-2

• ID: 5585
• GenBank: WP_248913285
• Name: mobQ_Ped0941_RS09555_pMGB0941-2
• UniProt ID: _
• Length: 687 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 687 a.a.
• Molecular weight: 80500.06 Da
• Isoelectric Point: 9.2567

>WP_248913285.1 MobQ family relaxase [Pediococcus pentosaceus]
MAIFHMSFSNISAGKGRSAIASAAYRSGEKLFDDKEGRHYFYARLVIPESFILTPKNSPEWASDREQLWN
EVEKKDRKSNSRYAKEFNVALPVELSESEQKELLTKYVQENFVDEGMVADVAIHRDHPDNPHAHVMLTNR
PFNSDGTWGIKSKKQYILDDNGNKTYTGTSKYPKSRKILMVDWDKKEKITEWRHNWAASVNEILEQKNIP
DRISEKSFIEQGIDDTPMQHEGINSKRHERKAFNQQVKNYRKSKAGYKNMQEKVVNRGHLDSLSKHFSFN
EKKVVKELSHELKTYISLESLDDKRRMLFNWKNSTLIKHAVGQDVTKQLLTINQQESSLKKADELLNKVV
DRTTKKLYPELNFEQTTQAERRELIKETDSEQTVFKGSELNERLMNIRDDLLTQQLLTFTKRPYVGWKLL
MQQEKEVKIELKYTLMIHDDNLESLEHVDQGLLEKYSPTEQQKITRAVKDLRTIMAVKQVIQTQYQEVLR
RAFPNGNFNELPMIKQEQAYTAVMYYDPVLKPCQAETIEQWQANPPQVFSPQEHQQGLAYLSGQLSLDQL
ENHHLQRVLKHDGTKQLFLGECKADPTIKNSQIEKIQKQLKGQQAKDDQYRKANIGHYQPLNYKPVSPDY
YLKTAFSDAIMTVLYARDEDYQRQRQAQGLKETEWEMTKKQRQHQTRNRHEDGGMHL

Protein domains

Predicted by InterproScan.

• Nicking enzyme C-terminal middle helical domain (NES_C_h) (276-384)

• MobA/MobL family (MobA_MobL) (17-241)

Protein structure

No available structure.

T4CP

t4cp1_Ped0941_RS09800_pMGB0941-2

• ID: 5962
• GenBank: WP_248913271
• Name: t4cp1_Ped0941_RS09800_pMGB0941-2
• UniProt ID: _
• Length: 583 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 583 a.a.
• Molecular weight: 67924.39 Da
• Isoelectric Point: 9.8397

>WP_248913271.1 type IV secretory system conjugative DNA transfer family protein [Pediococcus pentosaceus]
MAIKSIGSGVNRRKQNYAPWWARNIKSVNVFLGCVLIMIMLAFTYVSGMSYALFMAGRQWFINLMKNEQI
WTEPKQLGLTWHHVFDWHYVLPTIECHNPIFPYAVNFGSLTVVYMWWRIIYTYQIYNGYEKGAESFTTEE
QLKKEYAQIPDRKKGFEGYGGIPLVHFNNSVGSLIQTITYSPFFEIKIFNRKIFRSLRAMLIKFYDKFPG
GTHGIDQTTVNTMIYGITRSGKGQTIILPLIDILSRAFKKCSMFVNDPKSELYKMGTILLRLRGYRVFVL
NLQKMSKSMSYNPLQIIINYTKKGYYDEAQQEANRLSTAIYSNDNEKDPFWSNSSINLLNAMIFSQLDLA
ERHNSWNKVTMNNIYKQLTEMGGQEMPDPLIKGKTISKLTFYFDQMSKAPTSEFREMALQAFQQSRFAGS
ETHGSIYAGMMAGIKMYQQRDIARLTSMNSIDFRDAGFPRRISIRFPEQLTFQTENVIWRDIKANKTIEK
KTQEVDEHGYLEVPLKEKLNTKYQLEVNFDHKLTEEKYHKIKYVYSGKIMRKATVPNNFKLFKKVAGKIS
PDSSLSNVVEPEFDQQIEFTYSE

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (222-456)

Protein structure

No available structure.

t4cp2_Ped0941_RS09805_pMGB0941-2

• ID: 5963
• GenBank: WP_248913270
• Name: t4cp2_Ped0941_RS09805_pMGB0941-2
• UniProt ID: _
• Length: 304 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 304 a.a.
• Molecular weight: 34539.97 Da
• Isoelectric Point: 4.7488

>WP_248913270.1 TraG/TraD/VirD4 family protein [Pediococcus pentosaceus]
MAVFFVTPPHNPAYNQLVSFAIDQSFNQIYNMALGNEGKSYIRTHFIIDEGGQLPKIQDLPTKFSIGLGS
ELLFDFVLQNKGQLRILYSKEEAETIISNCGNTLYILSKDLETVKEISDEVGNTTVNMINRSTTPGMSTA
RDNLSNSREGVKLITPEELLRLKTGEMVVVRSTARTDVKNRIIRANPIFDTGRTRLPSAWQFLYSTFDSK
ATADDICIETPHKRLKLSDISYNYVKDDFSNKNEIDQMVELSSRGNQSEDYEETMPISEEEYKDMVAAMK
RKHELESQDQTRSMVDSMLDIQIS

Protein domains

Predicted by InterproScan.

• TraM recognition site of TraD and TraG (TraG-D_C) (43-163)

Protein structure

No available structure.

Host bacterium

• ID: 8785
• GenBank: NZ_CP085180
• Plasmid name: pMGB0941-2
• Incompatibility group: -
• Plasmid size: 51339 bp
• Coordinate of oriT [Strand]: 3430..3450 [-]
• Host baterium: Pediococcus pentosaceus strain MGB0941

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -