Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 108301
• Name: oriT_p1_EfsC49_repA_N
• Organism: Enterococcus faecalis strain EfsC49
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP124907 (56773..56822 [-], 50 nt)
• oriT length: 50 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 50 nt

>oriT_p1_EfsC49_repA_N
AATATCGCAACATGCTAGCATGTTGCTCTGCTCGCAAAAAGAAAGCCTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_QLQ59_RS13580_p1_EfsC49_repA_N

• ID: 5548
• GenBank: WP_010784125
• Name: Relaxase_QLQ59_RS13580_p1_EfsC49_repA_N
• UniProt ID: _
• Length: 561 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 561 a.a.
• Molecular weight: 65427.18 Da
• Isoelectric Point: 6.1241

>WP_010784125.1 relaxase/mobilization nuclease domain-containing protein [Enterococcus faecalis]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKTHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKRVFEQISDKIAAKEGAKIIEKSPKNS
HKKYTMWETESLYKEKIKSRLDFLLEQSSSINDFLEKAEALNLSVDFSKKWTTYRLLDEPQIKNTRSRSL
SKNDPTRYNYEKIVERLKENKSVLTLKEVVEKYVEKGEKSKNEFDYQLTIDEWQISHKTTRGYYVNVDFG
FGERGKVFIGAYKVEPLENGQYNIYVKRKDFFYLMNEKNADRNRYMTGETLIKQLRLYNGQTPLKKEPVM
RTIDELVNAINFLAANEIEDTRQLELLEEKLEAAFLEAEETLETLDEKMLELHQLSNLLLENELNGELEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQSLLENKLERTRNEINQLHEIQAVQKKETANQNNVKPK
L

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (37-296)

Protein structure

No available structure.

Auxiliary protein

WP_010784124_p1_EfsC49_repA_N

• ID: 2658
• GenBank: WP_010784124
• Name: WP_010784124_p1_EfsC49_repA_N
• UniProt ID: _
• Length: 118 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 118 a.a.
• Molecular weight: 13987.11 Da
• Isoelectric Point: 10.1993

>WP_010784124.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Enterococcus]
MENKQKLKRPIQRIVRLSEEENNLIKRKIEKSFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVHSLNALVQSELNKLIKRKEQS

Protein domains

Predicted by InterproScan.

• Bacterial mobilisation protein (MobC) (MobC) (64-105)

Protein structure

No available structure.

T4CP

t4cp2_QLQ59_RS13600_p1_EfsC49_repA_N

• ID: 5914
• GenBank: WP_010784122
• Name: t4cp2_QLQ59_RS13600_p1_EfsC49_repA_N
• UniProt ID: _
• Length: 609 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 609 a.a.
• Molecular weight: 70099.20 Da
• Isoelectric Point: 6.4057

>WP_010784122.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIISKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNVAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEVKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRALEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (144-558)

Protein structure

No available structure.

Host bacterium

• ID: 8736
• GenBank: NZ_CP124907
• Plasmid name: p1_EfsC49_repA_N
• Incompatibility group: -
• Plasmid size: 79359 bp
• Coordinate of oriT [Strand]: 56773..56822 [-]
• Host baterium: Enterococcus faecalis strain EfsC49

Cargo genes

• Drug resistance gene: ClpL
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA9, AcrIIA21