Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   108258
Name   oriT_pM7b in_silico
Organism   Klebsiella quasipneumoniae strain M7
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP124868 (30491..30579 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pM7b
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   5524 GenBank   WP_012783930
Name   nikB_QJS48_RS25795_pM7b insolico UniProt ID   N0GUP8
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104070.55 Da        Isoelectric Point: 7.3623

>WP_012783930.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSHMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLAERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWLPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALVDKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB N0GUP8


Auxiliary protein

ID   2638 GenBank   WP_001283947
Name   WP_001283947_pM7b insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   5891 GenBank   WP_012783931
Name   trbC_QJS48_RS25800_pM7b insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86911.02 Da        Isoelectric Point: 6.7713

>WP_012783931.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILTAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 35957..75188

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJS48_RS25800 (QJS48_25800) 33673..35964 - 2292 WP_012783931 F-type conjugative transfer protein TrbC -
QJS48_RS25805 (QJS48_25805) 35957..37027 - 1071 WP_012783932 IncI1-type conjugal transfer protein TrbB trbB
QJS48_RS25810 (QJS48_25810) 37046..38254 - 1209 WP_001703845 IncI1-type conjugal transfer protein TrbA trbA
QJS48_RS25815 (QJS48_25815) 38561..39340 - 780 WP_275450201 protein FinQ -
QJS48_RS25820 (QJS48_25820) 39967..40119 + 153 WP_001387489 Hok/Gef family protein -
QJS48_RS25825 (QJS48_25825) 40191..40442 - 252 WP_001291964 hypothetical protein -
QJS48_RS25830 (QJS48_25830) 40943..41038 + 96 WP_000609148 DinQ-like type I toxin DqlB -
QJS48_RS25835 (QJS48_25835) 41103..41279 - 177 WP_001054900 hypothetical protein -
QJS48_RS25840 (QJS48_25840) 41671..41880 + 210 WP_000062603 HEAT repeat domain-containing protein -
QJS48_RS25845 (QJS48_25845) 41952..42614 - 663 WP_012783935 plasmid IncI1-type surface exclusion protein ExcA -
QJS48_RS25850 (QJS48_25850) 42685..44853 - 2169 WP_015508354 DotA/TraY family protein traY
QJS48_RS25855 (QJS48_25855) 44950..45534 - 585 WP_012783937 conjugal transfer protein TraX -
QJS48_RS25860 (QJS48_25860) 45563..46765 - 1203 WP_012783938 IncI1-type conjugal transfer protein TraW traW
QJS48_RS25865 (QJS48_25865) 46732..47346 - 615 WP_000337394 IncI1-type conjugal transfer protein TraV traV
QJS48_RS25870 (QJS48_25870) 47346..50390 - 3045 WP_012783939 IncI1-type conjugal transfer protein TraU traU
QJS48_RS25875 (QJS48_25875) 50480..51280 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
QJS48_RS25880 (QJS48_25880) 51264..51452 - 189 WP_001277255 putative conjugal transfer protein TraS -
QJS48_RS25885 (QJS48_25885) 51516..51920 - 405 WP_000086965 IncI1-type conjugal transfer protein TraR traR
QJS48_RS25890 (QJS48_25890) 51971..52498 - 528 WP_001055569 conjugal transfer protein TraQ traQ
QJS48_RS25895 (QJS48_25895) 52498..53202 - 705 WP_001493811 IncI1-type conjugal transfer protein TraP traP
QJS48_RS25900 (QJS48_25900) 53202..54491 - 1290 WP_011264042 conjugal transfer protein TraO traO
QJS48_RS25905 (QJS48_25905) 54494..55477 - 984 WP_001191880 IncI1-type conjugal transfer protein TraN traN
QJS48_RS25910 (QJS48_25910) 55488..56180 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
QJS48_RS25915 (QJS48_25915) 56177..56524 - 348 WP_001055900 conjugal transfer protein traL
QJS48_RS25920 (QJS48_25920) 56542..60309 - 3768 WP_012783940 LPD7 domain-containing protein -
QJS48_RS25925 (QJS48_25925) 60399..60950 - 552 WP_012783942 phospholipase D family protein -
QJS48_RS25930 (QJS48_25930) 60965..61255 - 291 WP_001314267 hypothetical protein traK
QJS48_RS25935 (QJS48_25935) 61252..62400 - 1149 WP_001024971 plasmid transfer ATPase TraJ virB11
QJS48_RS25940 (QJS48_25940) 62397..63215 - 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
QJS48_RS25945 (QJS48_25945) 63212..63670 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
QJS48_RS25950 (QJS48_25950) 64064..64648 - 585 WP_000977522 histidine phosphatase family protein -
QJS48_RS25955 (QJS48_25955) 64708..65910 - 1203 WP_000976353 conjugal transfer protein TraF -
QJS48_RS25960 (QJS48_25960) 65996..66820 - 825 WP_001238932 conjugal transfer protein TraE traE
QJS48_RS25965 (QJS48_25965) 66971..68125 - 1155 WP_282863488 site-specific integrase -
QJS48_RS25970 (QJS48_25970) 69390..70682 - 1293 WP_001417545 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
QJS48_RS25975 (QJS48_25975) 70682..71338 - 657 WP_001193553 prepilin peptidase -
QJS48_RS25980 (QJS48_25980) 71323..71883 - 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
QJS48_RS25985 (QJS48_25985) 71893..72507 - 615 WP_001542527 type 4 pilus major pilin -
QJS48_RS25990 (QJS48_25990) 72525..73622 - 1098 WP_001492931 type II secretion system F family protein -
QJS48_RS25995 (QJS48_25995) 73635..75188 - 1554 WP_001492932 ATPase, T2SS/T4P/T4SS family virB11
QJS48_RS26000 (QJS48_26000) 75199..75651 - 453 WP_012783945 type IV pilus biogenesis protein PilP -
QJS48_RS26005 (QJS48_26005) 75638..76933 - 1296 WP_012783946 type 4b pilus protein PilO2 -
QJS48_RS26010 (QJS48_26010) 76926..78608 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
QJS48_RS26015 (QJS48_26015) 78622..79059 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
QJS48_RS26020 (QJS48_26020) 79059..80126 - 1068 WP_012783947 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   8693 GenBank   NZ_CP124868
Plasmid name   pM7b Incompatibility group   IncI1
Plasmid size   85705 bp Coordinate of oriT [Strand]   30491..30579 [-]
Host baterium   Klebsiella quasipneumoniae strain M7

Cargo genes

Drug resistance gene   blaCTX-M-55
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrVA2