Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   108257
Name   oriT_pM7a in_silico
Organism   Klebsiella quasipneumoniae strain M7
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP124867 (110164..110213 [-], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_pM7a
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   5523 GenBank   WP_172694377
Name   traI_QJS48_RS25560_pM7a insolico UniProt ID   _
Length   1753 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1753 a.a.        Molecular weight: 190750.85 Da        Isoelectric Point: 6.2896

>WP_172694377.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Klebsiella]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTGSSNMLTLKDREGVRLDLKVSAV
DSQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERYISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDLERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQLASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILE
PRNDRAVKSADLLFGRARPLDETAAGRAALQQSGLAQGNSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLTGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRTAEEQQRREMEKQAEQTAREVAGEARKAGEP
VDRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASETLQRDRLQAVERDMVRDLNREKTL
GGD

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(10-284)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(635-714)

AAA domain (AAA_30)

(971-1157)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(577-629)

DNA helicase TraI (TraI_C)

(1433-1555)


  Protein structure



No available structure.




T4CP

ID   5890 GenBank   WP_228719473
Name   traD_QJS48_RS25555_pM7a insolico UniProt ID   _
Length   770 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 770 a.a.        Molecular weight: 85966.07 Da        Isoelectric Point: 5.0099

>WP_228719473.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Klebsiella]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAEEPSVRATEPSVLRLTTVPLIKPKAAAAAAAAATASSAGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINYSHRHDEQDEVEIGGNF

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

F sex factor protein N terminal (TraD_N)

(32-128)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 109606..137800

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJS48_RS25345 (QJS48_25345) 105609..105965 + 357 WP_013023814 hypothetical protein -
QJS48_RS25350 (QJS48_25350) 106026..106238 + 213 WP_013023815 hypothetical protein -
QJS48_RS25355 (QJS48_25355) 106249..106473 + 225 WP_004152719 hypothetical protein -
QJS48_RS25360 (QJS48_25360) 106554..106874 + 321 WP_004152720 type II toxin-antitoxin system RelE/ParE family toxin -
QJS48_RS25365 (QJS48_25365) 106864..107142 + 279 WP_004152721 helix-turn-helix transcriptional regulator -
QJS48_RS25370 (QJS48_25370) 107143..107556 + 414 WP_013023817 helix-turn-helix domain-containing protein -
QJS48_RS25375 (QJS48_25375) 108390..109211 + 822 WP_004182076 DUF932 domain-containing protein -
QJS48_RS25380 (QJS48_25380) 109244..109573 + 330 WP_011977736 DUF5983 family protein -
QJS48_RS25385 (QJS48_25385) 109606..110091 - 486 WP_004178063 transglycosylase SLT domain-containing protein virB1
QJS48_RS25390 (QJS48_25390) 110526..110918 + 393 WP_004206766 conjugal transfer relaxosome DNA-binding protein TraM -
QJS48_RS25395 (QJS48_25395) 111125..111820 + 696 WP_023307551 transcriptional regulator TraJ family protein -
QJS48_RS25400 (QJS48_25400) 111904..112275 + 372 WP_004208838 TraY domain-containing protein -
QJS48_RS25405 (QJS48_25405) 112329..112697 + 369 WP_064147976 type IV conjugative transfer system pilin TraA -
QJS48_RS25410 (QJS48_25410) 112711..113016 + 306 WP_004178059 type IV conjugative transfer system protein TraL traL
QJS48_RS25415 (QJS48_25415) 113036..113602 + 567 WP_004152602 type IV conjugative transfer system protein TraE traE
QJS48_RS25420 (QJS48_25420) 113589..114329 + 741 WP_004152497 type-F conjugative transfer system secretin TraK traK
QJS48_RS25425 (QJS48_25425) 114329..115753 + 1425 WP_040088218 F-type conjugal transfer pilus assembly protein TraB traB
QJS48_RS25430 (QJS48_25430) 115746..116159 + 414 Protein_133 conjugal transfer pilus-stabilizing protein TraP -
QJS48_RS25435 (QJS48_25435) 116196..116351 + 156 WP_172694479 hypothetical protein -
QJS48_RS25440 (QJS48_25440) 116371..116940 + 570 WP_015065628 type IV conjugative transfer system lipoprotein TraV traV
QJS48_RS25445 (QJS48_25445) 117096..117494 + 399 WP_223177007 hypothetical protein -
QJS48_RS25450 (QJS48_25450) 117523..117810 + 288 WP_052451814 hypothetical protein -
QJS48_RS25455 (QJS48_25455) 117834..118052 + 219 WP_004171484 hypothetical protein -
QJS48_RS25460 (QJS48_25460) 118053..118370 + 318 WP_016530481 hypothetical protein -
QJS48_RS25465 (QJS48_25465) 118437..118841 + 405 WP_172694383 hypothetical protein -
QJS48_RS25470 (QJS48_25470) 118866..119264 + 399 WP_015065631 hypothetical protein -
QJS48_RS25475 (QJS48_25475) 119336..121975 + 2640 WP_032456787 type IV secretion system protein TraC virb4
QJS48_RS25480 (QJS48_25480) 121975..122364 + 390 WP_004152506 type-F conjugative transfer system protein TrbI -
QJS48_RS25485 (QJS48_25485) 122364..122990 + 627 WP_172694382 type-F conjugative transfer system protein TraW traW
QJS48_RS25490 (QJS48_25490) 123034..123993 + 960 WP_267313299 conjugal transfer pilus assembly protein TraU traU
QJS48_RS25495 (QJS48_25495) 124006..124644 + 639 WP_015065635 type-F conjugative transfer system pilin assembly protein TrbC trbC
QJS48_RS25500 (QJS48_25500) 124703..126658 + 1956 WP_087749112 type-F conjugative transfer system mating-pair stabilization protein TraN traN
QJS48_RS25505 (QJS48_25505) 126690..126926 + 237 WP_040088231 conjugal transfer protein TrbE -
QJS48_RS26615 126923..127108 + 186 WP_040088233 hypothetical protein -
QJS48_RS25510 (QJS48_25510) 127154..127480 + 327 WP_004152685 hypothetical protein -
QJS48_RS25515 (QJS48_25515) 127501..128253 + 753 WP_015065637 type-F conjugative transfer system pilin assembly protein TraF traF
QJS48_RS25520 (QJS48_25520) 128264..128503 + 240 WP_004152687 type-F conjugative transfer system pilin chaperone TraQ -
QJS48_RS25525 (QJS48_25525) 128475..129047 + 573 WP_023292147 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
QJS48_RS25530 (QJS48_25530) 129040..129468 + 429 WP_040088240 conjugal transfer protein TrbF -
QJS48_RS25535 (QJS48_25535) 129455..130825 + 1371 WP_172694380 conjugal transfer pilus assembly protein TraH traH
QJS48_RS25540 (QJS48_25540) 130825..133674 + 2850 WP_172694379 conjugal transfer mating-pair stabilization protein TraG traG
QJS48_RS25545 (QJS48_25545) 133680..134207 + 528 WP_016528837 conjugal transfer entry exclusion protein TraS -
QJS48_RS25550 (QJS48_25550) 134397..135128 + 732 WP_004152629 conjugal transfer complement resistance protein TraT -
QJS48_RS25555 (QJS48_25555) 135488..137800 + 2313 WP_228719473 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   8692 GenBank   NZ_CP124867
Plasmid name   pM7a Incompatibility group   IncR
Plasmid size   147040 bp Coordinate of oriT [Strand]   110164..110213 [-]
Host baterium   Klebsiella quasipneumoniae strain M7

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   hdt
Symbiosis gene   -
Anti-CRISPR   AcrIE9