Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 108247
• Name: oriT_p1_EfsC94_repUS11
• Organism: Enterococcus faecalis strain EfsC94
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP124902 (9273..9322 [+], 50 nt)
• oriT length: 50 nt
• IRs (inverted repeats): _
• Location of nic site: 34..35
• Conserved sequence flanking the nic site: GCTTGCAAAA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 50 nt

>oriT_p1_EfsC94_repUS11
AATATCGCAACATGCTAGCATGTTGCTCCGCTTGCAAAAAGAAAGCCTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_QLQ35_RS13905_p1_EfsC94_repUS11

• ID: 5516
• GenBank: WP_161323729
• Name: Relaxase_QLQ35_RS13905_p1_EfsC94_repUS11
• UniProt ID: _
• Length: 561 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 561 a.a.
• Molecular weight: 65321.94 Da
• Isoelectric Point: 6.5183

>WP_161323729.1 relaxase/mobilization nuclease domain-containing protein [Enterococcus faecalis]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGKYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKRVFEQISDKIAAKEGAKIIEKSPKQT
HTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYG
YGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVM
QTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSNLLLENELQGDPEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQSLLENKLERTRNEINQLHEIQAVQKKETANQNNVKPK
L

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (37-296)

Protein structure

No available structure.

Auxiliary protein

WP_002393737_p1_EfsC94_repUS11

• ID: 2633
• GenBank: WP_002393737
• Name: WP_002393737_p1_EfsC94_repUS11
• UniProt ID: A0A8E7CBI6
• Length: 118 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 118 a.a.
• Molecular weight: 13965.01 Da
• Isoelectric Point: 9.6545

>WP_002393737.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Enterococcus]
MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKDQS

Protein domains

Predicted by InterproScan.

• Bacterial mobilisation protein (MobC) (MobC) (64-105)

Protein structure

No available structure.

T4CP

t4cp2_QLQ35_RS13885_p1_EfsC94_repUS11

• ID: 5884
• GenBank: WP_282861521
• Name: t4cp2_QLQ35_RS13885_p1_EfsC94_repUS11
• UniProt ID: _
• Length: 609 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 609 a.a.
• Molecular weight: 70193.23 Da
• Isoelectric Point: 6.2947

>WP_282861521.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQTYKKELKPYLYLGVFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGVLGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNYYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (144-558)

Protein structure

No available structure.

Host bacterium

• ID: 8682
• GenBank: NZ_CP124902
• Plasmid name: p1_EfsC94_repUS11
• Incompatibility group: -
• Plasmid size: 78787 bp
• Coordinate of oriT [Strand]: 9273..9322 [+]
• Host baterium: Enterococcus faecalis strain EfsC94

Cargo genes

• Drug resistance gene: -
• Virulence gene: prgB/asc10
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -