Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 108011
• Name: oriT_JICSp1
• Organism: Staphylococcus aureus strain JICS127
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_AP025694 (19219..19407 [+], 189 nt)
• oriT length: 189 nt
• IRs (inverted repeats): 149..155, 162..168 (CTATCAT..ATGATAG) 132..138, 142..148 (GTCTGGC..GCCAGAC) 62..68, 79..85 (TAGTGTC..GACACTA) 33..38, 45..50 (TGTCAC..GTGACA)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 189 nt

>oriT_JICSp1
TGTCACAAAAATAAGACAAAATCTACATGTAGTGTCACAAAACCGTGACAAACACAATATATAGTGTCGCAAAAATGTGACACTAGGTGTTTTTATGATATCACTATGAAATGTCCTAAAACCCTTGGAATGTCTGGCTTTGCCAGACCTATCATTGTACGATGATAGCAAATTCCCCTTATGCTCTTA

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobP2_M2038_RS14565_JICSp1

• ID: 5368
• GenBank: WP_248316377
• Name: mobP2_M2038_RS14565_JICSp1
• UniProt ID: _
• Length: 383 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 383 a.a.
• Molecular weight: 45939.31 Da
• Isoelectric Point: 9.8763

>WP_248316377.1 MobP2 family relaxase [Staphylococcus aureus]
MMPKIVTVSQFEQPNEFYEYSNYVNYMKRGEAQNNKDDFKYDVYAHYMFDEEKSQNMFNEHDNFMSKKAI
NKIKSDFSHAQKNKGMMWKDVISFDNSSLESVGIYDSKTHTLDEQKIKEATRKMMKRFEKKEGLEDNLVW
SGAIHYNTDNIHVHVAAVEKLIKRTRGKRKPATLKHMKSTFANELFDIKGERQKINEFIRERIVKGIRDE
SEPCKDKAMKKQIKKVHKLLEDIPKNQWNYNNNILKYIRPEIDKITDIYIQNYHSKDFEVFKNDLKRQNN
LYKETYGENSNYGSYEKTKMKDLYARSGNTILKNIKELDQDLMKVKQQFTHSKLNVTITKLKIGKCLNQA
LYRTKYALRSDYDKEKNIQQYYQEFDKQSYRER

Protein domains

Predicted by InterproScan.

• MobL relaxases (MobL) (2-351)

Protein structure

No available structure.

T4CP

t4cp2_M2038_RS14515_JICSp1

• ID: 5662
• GenBank: WP_248316376
• Name: t4cp2_M2038_RS14515_JICSp1
• UniProt ID: _
• Length: 923 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 923 a.a.
• Molecular weight: 106273.29 Da
• Isoelectric Point: 7.3536

>WP_248316376.1 VirD4-like conjugal transfer protein, CD1115 family [Staphylococcus aureus]
MRFGSKDKHGYRRKKLSFSQNKHLLAHPCILVSILLLIMLLATAIANFLINILMTVFFGNHDIFTNMKQL
NTKYTDYIFNFRSDAVLLYIPIWVLVFIFFGKKVYQFRQRFKSLNNNEKASGRFATRREITQQYKAIPQR
EHAFEGEGGLPVAMFGVYDFVTKTKQYLEQRKTAIREINEITDLTADEKLELRKDKLGKMNKEFATTFFS
KREFTFIDESPTNNLIVGTSRSGKGEIVVLSMIENYSRSSEQPSLIVNDMKGELFSASYKTLENRGYQVE
IFNLDQPMESTMSFNPLQQVIDEYMKGDLGEAQEMTKQITYTLTNNEGVEDNEWNLMAGALINAMILALC
EETLPKNPEKVTLYSVSNMLQTLSTKRFYKEIAIGNKVQLIEVSALDEYMERFPSHSPAKTQYATVQAAP
DKMRSSIIGTALKALQPFTTDTIAKLTSHTSFDINKLGFPSIIDGYATKDSTFELGVQVLRDGENGIEYE
EVEGIQIGVNEYGYWQYPFKTVLKVGDRIETIEKDENNQVITSYFYVEHIDDKGQVAFNQKGELDNSDIT
IRKFNSFPKPIAVFMVVPDYNNANHGIASILVNQIVFEISKNSQLYTENQSTYRRVIFHLDEAGNMPPIP
NLSQKVNVSLSRGLRFNFFVQAFSQFKDKYGDAYDAIMDACQNKVYIMATQEQTLKDFSAMIGSQQITVH
SRSGEAGAIKSSITENQEERPLLRPDELARLQEGETVVVRNLKRQDKKRNKVMSYPIFNDGKYAMKYRYQ
YLSDLMDTSQSIIHFRPMLKARCEHRHLQLEATLVDWDKLIEEMQEGIDGQKEENKNINDINQYKKSNEE
GNVAVRVKRLETLKDKVGDAVFDKITRRFERYLRTYAEQGKNVNTITQKKLEELVLADSEVKEEEKTKRI
EMIKKLIKEAKTT

Protein domains

Predicted by InterproScan.

• TraM recognition site of TraD and TraG (TraG-D_C) (615-732)

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (209-458)

Protein structure

No available structure.

Host bacterium

• ID: 8446
• GenBank: NZ_AP025694
• Plasmid name: JICSp1
• Incompatibility group: -
• Plasmid size: 40253 bp
• Coordinate of oriT [Strand]: 19219..19407 [+]
• Host baterium: Staphylococcus aureus strain JICS127

Cargo genes

• Drug resistance gene: aac(6')-aph(2'')
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21, AcrIIA14