Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 107982
• Name: oriT_32-5/1|unnamed2
• Organism: Rhizobium sp. 32-5/1
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP120640 (256736..256771 [-], 36 nt)
• oriT length: 36 nt
• IRs (inverted repeats): 22..28, 30..36 (CGTCGCT..AGCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 36 nt

>oriT_32-5/1|unnamed2
GGTTCCAAGGGCGCAATTATACGTCGCTGAGCGACG

Relaxase

traA_P6U16_RS28185_32-5/1|unnamed2

• ID: 5355
• GenBank: WP_277704963
• Name: traA_P6U16_RS28185_32-5/1|unnamed2
• UniProt ID: _
• Length: 1100 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1100 a.a.
• Molecular weight: 123461.12 Da
• Isoelectric Point: 10.1135

>WP_277704963.1 Ti-type conjugative transfer relaxase TraA [Rhizobium sp. 32-5/1]
MAIAHFSASIVSRGDGRSAVLSAAYRHCAKMEYEREAHSIDYTRKQGLLHEEFVLPADAPKWARALIADR
SVSGAVEAFWNKVEAFEKRSDAQLARDLTIALPLELNPEQNIALIRDFVEKHILAKGMVADWVYHDNPGN
PHIHLMTTLRPLTEDGFGAKKVAVIGEDGRPVRTKSGKIVYELWAGSTDDFNKLRDGWFERLNHHLVLCG
VDLRIDGRSYEKQGIDLEPTIHLGVGAKAIERKAESRGVRPELERIELNEQRRSENARRILRNPGIVLDL
ITREKSVFDERDVAKVLHRYIDDPVMFQQLLARIQQGPETLRLQRDTVEFATGQRLPARYTTRELIRLEA
EMARRSLWLSERENHGVSQAVIEATFVRHTRLSHEQRTAIEHVAGSARIAAVVGRAGAGKTTMMKAAREA
WELAGYRVVGGALAGKAAEGLEKEAGIQSRTLASWELRWKKNRDTLDAKTVFVMDEAGMVASRQMAGFVE
AVVKSGAKLVLIGDPEQLQPIEAGAAFRAIADRVGYAELETIYRQRDDWMRKASLDLARGRVGDALAAYR
LEGRVLGSELKAQAVEHLIADWNRDYDPAKSMLMLAHLRRDVRILNVLAREKLVERGILSEGHVFKTADG
IRHFDAGDQIVFLKNEGALGVKNGMIGRVVEAAPNRISVVVGEGDQRRQVSVEQRFYTNLDHGYATTVHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHREDLTLYYGRRSFAKAGGLTEILSRRNAKETTLDYERGGLY
RPALAFAENRGLHIVQVARTLLRDRLDWTLRQKSKLFDLAARLRTAGTRLGLLQTPKPQTIKETRPMVSG
VKLFPVPLSDAVDRKVADDPAVKKQWEEVSTRFRYVFADPETAFRAMNFDAVFADKQVASRTLDKLAIDP
ASIGALKGKTGILASKSDREARRIADVNAPALKRDIETYLRIREVAVQWIETEEKTMRQRVSIDIPALSP
AAQSMLERVRDAIDRNDLPAAMAYALSNREAKTEIDGLNKAVTGRFGERTLLANSARNPQGQLFDKLSEG
LAPQEKERLKEAWPVMRTAQQLAAHERTVQSLRQAENIRLTQRQSSVLKQ

Protein domains

Predicted by InterproScan.

• AAA domain (AAA_30) (382-565)

• Viral (Superfamily 1) RNA helicase (Viral_helicase1) (692-736)

• MobA/MobL family (MobA_MobL) (17-243)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (855-951)

Protein structure

No available structure.

T4CP

traG_P6U16_RS28200_32-5/1|unnamed2

• ID: 5645
• GenBank: WP_277704964
• Name: traG_P6U16_RS28200_32-5/1|unnamed2
• UniProt ID: _
• Length: 656 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 656 a.a.
• Molecular weight: 70174.08 Da
• Isoelectric Point: 9.4780

>WP_277704964.1 Ti-type conjugative transfer system protein TraG [Rhizobium sp. 32-5/1]
MTMNRLFLSVVPAVIIIAIMFASSGLEHRLGGFGSSAQTKLLLGRAGLALPYVAAAAIGVISLFAANGSS
NIKTAALSVLAGNGAVIVIAVAREATRLAGIASSVPAEQSVLAYTDPATMVGASAALFSGVFAVRVAIKG
NAAFTQAGPTRIGGKRAVHGDANWMTMQEAGKLFSDVGGIVVGERYRVDRDSVAAMAFRTDDPRSWGSGG
KSPLLCFDGSFGSSHGIVFAGSGGFKTTSVTIPTALKWGGGLVVLDPSSEVAPMVVEHRRKAGRKVIVLD
PVNPATGFNALDWIGCFGGSKEEDIVAVATWIMTDNARAASARDDFFRASAMQLLTALMADVCLSGHTDA
KDQTLRRVRANLSEPEPKLRERLTRIYEQSESTFVRENVAVFVNMTPETFSGVYANAVKETHWLSYTNYA
AIVSGNSFSTDELAAGVTDIFIALDLKVLEAHPGLARVVIGSFLNAIYNRNGEVAGRTLFLLDEVARLGY
LRILETARDAGRKYGISLALIFQSIGQMREAYGGRDASSKWFESASWISFAAINDPETAEYISKRCGDTT
VEVDQTSRSSGMKGSSRSRSKQLTRRPLILPHEVLRMRGDEQIVFTAGNAPLRCGRAIWFRRKDMSASVG
ENRFHKQATDGVKSEKAAPTTDIEEK

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (163-638)

Protein structure

No available structure.

Host bacterium

• ID: 8417
• GenBank: NZ_CP120640
• Plasmid name: 32-5/1|unnamed2
• Incompatibility group: -
• Plasmid size: 265073 bp
• Coordinate of oriT [Strand]: 256736..256771 [-]
• Host baterium: Rhizobium sp. 32-5/1

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: fixA, fixB
• Anti-CRISPR: -