Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 107977 |
| Name | oriT_pEmeITTGR7a  |
| Organism | Sinorhizobium mexicanum strain ITTG R7 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP041239 (411627..411670 [+], 44 nt) |
| oriT length | 44 nt |
| IRs (inverted repeats) | 23..28, 33..38 (CGTCGC..GCGACG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 44 nt
>oriT_pEmeITTGR7a
GGATCCGAAGGGCGCAATTATACGTCGCTGATGCGACGCTCTGC
GGATCCGAAGGGCGCAATTATACGTCGCTGATGCGACGCTCTGC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 5639 | GenBank | WP_180941746 |
| Name | traG_FKV68_RS22075_pEmeITTGR7a |
UniProt ID | _ |
| Length | 658 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 658 a.a. Molecular weight: 71062.07 Da Isoelectric Point: 8.8478
>WP_180941746.1 Ti-type conjugative transfer system protein TraG [Sinorhizobium mexicanum]
MNRIMLFIAPVALIFLVMIGLTGVEHWLSGFGKTEAARQTLGRAGIALPYVAAAAIGIVFLFASAGAIRI
KTAGWGVAAGGIATIIVAIARETTRLSGLADQIPAGKSLVSYVDPATLIGAAAAALSACFALRVALAGNA
VFASAEPKRIRGKRALHGEAEWMKMQEAANLFSDAGGIVIGERYRVDRDSTAALAFRADNAETWGAGGKS
PLLCFDGSFGSSHGIVFAGSGGFKTTSVTIPTALKWGGSLIVLDPSNEVAPMVSVHRGSTNRDLFVLDPK
EPETGFNALDWIGQFGGTKEEDIASVASWIMSDSGGTRGVRDDFFRASALQLLTAMIADVCLSGHTEKEN
QTLRQVRANLSEPEPQLRQRLQEIYDNSESDFVKENVAAFVNMTPETFSGVYANAIKETHWLSYPNYAAL
VSGKTFSTGDLAEGNTDVFINIDLRTLETHAGLARVIIGSFLNGIYNRDGSMKGRALFLLDEVARLGYMR
ILETARDAGRKYGITLVMIYQSIGQLRETYGGRDASSKWFESASWISFAAINDPETADYISRRCGMTTVE
IDQVSRSSQMKGSSRTRSKQLAARPLIQPHEVLRMRADEQIVFTAGNAPLRCGRAIWFRRDDMKACVGMN
RFHRPRNTLGASRIEPVRSATSKADLGQ
MNRIMLFIAPVALIFLVMIGLTGVEHWLSGFGKTEAARQTLGRAGIALPYVAAAAIGIVFLFASAGAIRI
KTAGWGVAAGGIATIIVAIARETTRLSGLADQIPAGKSLVSYVDPATLIGAAAAALSACFALRVALAGNA
VFASAEPKRIRGKRALHGEAEWMKMQEAANLFSDAGGIVIGERYRVDRDSTAALAFRADNAETWGAGGKS
PLLCFDGSFGSSHGIVFAGSGGFKTTSVTIPTALKWGGSLIVLDPSNEVAPMVSVHRGSTNRDLFVLDPK
EPETGFNALDWIGQFGGTKEEDIASVASWIMSDSGGTRGVRDDFFRASALQLLTAMIADVCLSGHTEKEN
QTLRQVRANLSEPEPQLRQRLQEIYDNSESDFVKENVAAFVNMTPETFSGVYANAIKETHWLSYPNYAAL
VSGKTFSTGDLAEGNTDVFINIDLRTLETHAGLARVIIGSFLNGIYNRDGSMKGRALFLLDEVARLGYMR
ILETARDAGRKYGITLVMIYQSIGQLRETYGGRDASSKWFESASWISFAAINDPETADYISRRCGMTTVE
IDQVSRSSQMKGSSRTRSKQLAARPLIQPHEVLRMRADEQIVFTAGNAPLRCGRAIWFRRDDMKACVGMN
RFHRPRNTLGASRIEPVRSATSKADLGQ
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 5640 | GenBank | WP_180941763 |
| Name | t4cp2_FKV68_RS22175_pEmeITTGR7a |
UniProt ID | _ |
| Length | 818 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 818 a.a. Molecular weight: 92196.03 Da Isoelectric Point: 5.8989
>WP_180941763.1 conjugal transfer protein TrbE [Sinorhizobium mexicanum]
MVALKRFRATGPSFADLVPYAGLVDNGVLLLKDGSLMAGWYFAGPDSESATDLERSELSRQINAILSRLG
SGWMIQVEAIRIPTFDYPSEERCHFPDAVTRAIDAERRAHFTRERGHFESKHALILTYRPLESKRTALNK
YIYSDEESRKKTYADTVLFIFKNAIREIEQYFANTLSIRRMETREVAERGGERVARYDELLQFIRFCITG
ENHPIRLPDAAMYLDWIATAELEHGLTPKVENRFLGVVAIDGLPAESWPGILNSLDLMPLTYRWSSRFIF
LDAEEARQKLERTRKKWQQKVRPFFDQLFQTQSRSVDQDAMTMVAETEDAIAQASSQLVAYGYYTPVIIL
FESDREALREKAEAIRRLIQAEGFGSRIETLNATEAYLGSLPGNWYCNIREPLINTRNLADLIPLNSVWS
GSPAAPCPFYPPNSPPLMQVASGSTPFRLNLHVDDVGHTLIFGPTGSGKSTLLALIAAQFRRYERAQIFA
FDKGNSLLPLTLAAHGDHYEIGGDNADEGKVLAFCPLSDLESEPDRAWATEWIEMLVALQGVSVTPDHRN
AISRQVGLMASAPGRSLSDFVSGVQMREIKDALHHYTVDGPMGQLLDADEDGLTLGAFQTFEIEQLMNMG
ERNLVPVLTYLFRRIEKRLDGSPSLIVLDEAWLMLGHPVFRSKIREWLKVLRKSNCAVVLATQSISDAER
SGIIDVLKESCPTKICLPNGAAREPGTREFYERIGFNERQIEIVSNAIPKREYYVVTPEGRRLFDMALGP
LALSFVGASGKEDLKRIRALNSEHGRDWPIHWLQTRGVHDAASLLNFE
MVALKRFRATGPSFADLVPYAGLVDNGVLLLKDGSLMAGWYFAGPDSESATDLERSELSRQINAILSRLG
SGWMIQVEAIRIPTFDYPSEERCHFPDAVTRAIDAERRAHFTRERGHFESKHALILTYRPLESKRTALNK
YIYSDEESRKKTYADTVLFIFKNAIREIEQYFANTLSIRRMETREVAERGGERVARYDELLQFIRFCITG
ENHPIRLPDAAMYLDWIATAELEHGLTPKVENRFLGVVAIDGLPAESWPGILNSLDLMPLTYRWSSRFIF
LDAEEARQKLERTRKKWQQKVRPFFDQLFQTQSRSVDQDAMTMVAETEDAIAQASSQLVAYGYYTPVIIL
FESDREALREKAEAIRRLIQAEGFGSRIETLNATEAYLGSLPGNWYCNIREPLINTRNLADLIPLNSVWS
GSPAAPCPFYPPNSPPLMQVASGSTPFRLNLHVDDVGHTLIFGPTGSGKSTLLALIAAQFRRYERAQIFA
FDKGNSLLPLTLAAHGDHYEIGGDNADEGKVLAFCPLSDLESEPDRAWATEWIEMLVALQGVSVTPDHRN
AISRQVGLMASAPGRSLSDFVSGVQMREIKDALHHYTVDGPMGQLLDADEDGLTLGAFQTFEIEQLMNMG
ERNLVPVLTYLFRRIEKRLDGSPSLIVLDEAWLMLGHPVFRSKIREWLKVLRKSNCAVVLATQSISDAER
SGIIDVLKESCPTKICLPNGAAREPGTREFYERIGFNERQIEIVSNAIPKREYYVVTPEGRRLFDMALGP
LALSFVGASGKEDLKRIRALNSEHGRDWPIHWLQTRGVHDAASLLNFE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 416779..431102
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| FKV68_RS22095 (FKV68_22530) | 415042..415608 | + | 567 | WP_180941749 | conjugative transfer signal peptidase TraF | - |
| FKV68_RS22100 (FKV68_22535) | 415598..416761 | + | 1164 | WP_180941750 | conjugal transfer protein TraB | - |
| FKV68_RS22105 (FKV68_22540) | 416779..417387 | + | 609 | WP_180941963 | TraH family protein | virB1 |
| FKV68_RS22110 (FKV68_22545) | 417506..418759 | + | 1254 | WP_180941751 | ATP-binding protein | - |
| FKV68_RS22115 (FKV68_22550) | 418823..419010 | - | 188 | Protein_394 | DUF5615 family PIN-like protein | - |
| FKV68_RS22120 (FKV68_22555) | 419007..419636 | - | 630 | WP_180941752 | DUF433 domain-containing protein | - |
| FKV68_RS22125 (FKV68_22560) | 419810..420022 | - | 213 | WP_180941753 | helix-turn-helix transcriptional regulator | - |
| FKV68_RS22130 (FKV68_22565) | 420234..420551 | + | 318 | WP_180941754 | transcriptional repressor TraM | - |
| FKV68_RS22135 (FKV68_22570) | 420555..421259 | - | 705 | WP_180941755 | autoinducer binding domain-containing protein | - |
| FKV68_RS22140 (FKV68_22575) | 421583..422878 | - | 1296 | WP_180941756 | IncP-type conjugal transfer protein TrbI | virB10 |
| FKV68_RS22145 (FKV68_22580) | 422890..423327 | - | 438 | WP_180941757 | conjugal transfer protein TrbH | - |
| FKV68_RS22150 (FKV68_22585) | 423331..424143 | - | 813 | WP_180941758 | P-type conjugative transfer protein TrbG | virB9 |
| FKV68_RS22155 (FKV68_22590) | 424160..424822 | - | 663 | WP_180941759 | conjugal transfer protein TrbF | virB8 |
| FKV68_RS22160 (FKV68_22595) | 424847..426022 | - | 1176 | WP_180941760 | P-type conjugative transfer protein TrbL | virB6 |
| FKV68_RS22165 (FKV68_22600) | 426016..426216 | - | 201 | WP_180941761 | entry exclusion protein TrbK | - |
| FKV68_RS22170 (FKV68_22605) | 426213..427016 | - | 804 | WP_180941762 | P-type conjugative transfer protein TrbJ | virB5 |
| FKV68_RS22175 (FKV68_22610) | 426988..429444 | - | 2457 | WP_180941763 | conjugal transfer protein TrbE | virb4 |
| FKV68_RS22180 (FKV68_22615) | 429457..429756 | - | 300 | WP_180941764 | conjugal transfer protein TrbD | virB3 |
| FKV68_RS22185 (FKV68_22620) | 429749..430135 | - | 387 | WP_180941765 | TrbC/VirB2 family protein | virB2 |
| FKV68_RS22190 (FKV68_22625) | 430125..431102 | - | 978 | WP_180941766 | P-type conjugative transfer ATPase TrbB | virB11 |
| FKV68_RS22195 (FKV68_22630) | 431113..431739 | - | 627 | WP_180941767 | acyl-homoserine-lactone synthase | - |
| FKV68_RS22200 (FKV68_22640) | 432543..433766 | + | 1224 | WP_180941768 | plasmid partitioning protein RepA | - |
| FKV68_RS22205 (FKV68_22645) | 433823..434803 | + | 981 | WP_180941769 | plasmid partitioning protein RepB | - |
Host bacterium
| ID | 8412 | GenBank | NZ_CP041239 |
| Plasmid name | pEmeITTGR7a | Incompatibility group | - |
| Plasmid size | 436172 bp | Coordinate of oriT [Strand] | 411627..411670 [+] |
| Host baterium | Sinorhizobium mexicanum strain ITTG R7 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | ncrA |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA7 |