Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107945
Name   oriT_FDAARGOS_645|unnamed1 in_silico
Organism   Providencia stuartii strain FDAARGOS_645
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP044075 (39543..39693 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_FDAARGOS_645|unnamed1
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   5332 GenBank   WP_282553037
Name   mobH_FOB53_RS00250_FDAARGOS_645|unnamed1 insolico UniProt ID   _
Length   1012 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1012 a.a.        Molecular weight: 111990.11 Da        Isoelectric Point: 5.0936

>WP_282553037.1 MULTISPECIES: MobH family relaxase [Providencia]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNETWQWVMTRSPTRCRGGPTMKKML

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4CP

ID   5601 GenBank   WP_000178857
Name   traD_FOB53_RS00255_FDAARGOS_645|unnamed1 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69225.71 Da        Isoelectric Point: 6.9403

>WP_000178857.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKVKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-299)

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

  Protein structure



No available structure.



ID   5602 GenBank   WP_000637384
Name   traC_FOB53_RS00340_FDAARGOS_645|unnamed1 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92604.86 Da        Isoelectric Point: 5.9216

>WP_000637384.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTDRIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSQGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRRQAA

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

TraG P-loop domain (P-loop_TraG)

(449-809)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 37627..64002

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOB53_RS00240 (FOB53_00240) 33658..33891 - 234 WP_001191890 hypothetical protein -
FOB53_RS00245 (FOB53_00245) 33873..34490 - 618 WP_001249395 hypothetical protein -
FOB53_RS00250 (FOB53_00250) 34658..37630 + 2973 WP_001326173 MobH family relaxase -
FOB53_RS00255 (FOB53_00255) 37627..39492 + 1866 WP_000178857 conjugative transfer system coupling protein TraD virb4
FOB53_RS22140 (FOB53_00260) 39542..40087 + 546 WP_000228720 hypothetical protein -
FOB53_RS00265 (FOB53_00265) 40044..40673 + 630 WP_000743449 DUF4400 domain-containing protein tfc7
FOB53_RS22145 40721..40897 + 177 WP_169342285 hypothetical protein -
FOB53_RS00275 (FOB53_00275) 41139..41516 + 378 WP_000869297 hypothetical protein -
FOB53_RS00280 (FOB53_00280) 41516..42178 + 663 WP_001231464 hypothetical protein -
FOB53_RS21360 42359..42502 + 144 WP_001275801 hypothetical protein -
FOB53_RS00285 (FOB53_00285) 42514..42879 + 366 WP_001052530 hypothetical protein -
FOB53_RS00290 (FOB53_00290) 43024..43305 + 282 WP_000805625 type IV conjugative transfer system protein TraL -
FOB53_RS00295 (FOB53_00295) 43302..43928 + 627 WP_001049717 TraE/TraK family type IV conjugative transfer system protein traE
FOB53_RS00300 (FOB53_00300) 43912..44829 + 918 WP_000794249 type-F conjugative transfer system secretin TraK -
FOB53_RS00305 (FOB53_00305) 44829..46142 + 1314 WP_024131605 TraB/VirB10 family protein -
FOB53_RS00310 (FOB53_00310) 46139..46717 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
FOB53_RS00315 (FOB53_00315) 46721..47113 + 393 WP_000479535 TraA family conjugative transfer protein -
FOB53_RS22150 (FOB53_00320) 47314..47928 + 615 Protein_63 DUF4165 domain-containing protein -
FOB53_RS00330 (FOB53_00330) 49158..54032 + 4875 Protein_65 Ig-like domain-containing protein -
FOB53_RS00335 (FOB53_00335) 54181..54888 + 708 WP_001259346 DsbC family protein -
FOB53_RS00340 (FOB53_00340) 54885..57332 + 2448 WP_000637384 type IV secretion system protein TraC virb4
FOB53_RS00345 (FOB53_00345) 57347..57664 + 318 WP_000351984 hypothetical protein -
FOB53_RS00350 (FOB53_00350) 57661..58191 + 531 WP_001010740 S26 family signal peptidase -
FOB53_RS00355 (FOB53_00355) 58154..59419 + 1266 WP_001447719 TrbC family F-type conjugative pilus assembly protein -
FOB53_RS00360 (FOB53_00360) 59416..60087 + 672 WP_000575345 EAL domain-containing protein -
FOB53_RS00365 (FOB53_00365) 60087..61091 + 1005 WP_005507569 TraU family protein -
FOB53_RS00370 (FOB53_00370) 61195..64002 + 2808 WP_001447718 conjugal transfer mating pair stabilization protein TraN traN
FOB53_RS00375 (FOB53_00375) 64041..64682 - 642 WP_223854615 hypothetical protein -
FOB53_RS00380 (FOB53_00380) 65024..65665 - 642 WP_000796664 hypothetical protein -
FOB53_RS00385 (FOB53_00385) 65960..66280 + 321 WP_000547566 hypothetical protein -
FOB53_RS00390 (FOB53_00390) 66584..66769 + 186 WP_001186917 hypothetical protein -
FOB53_RS00395 (FOB53_00395) 66989..67957 + 969 WP_000085160 AAA family ATPase -
FOB53_RS00400 (FOB53_00400) 67968..68876 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   8380 GenBank   NZ_CP044075
Plasmid name   FDAARGOS_645|unnamed1 Incompatibility group   IncA/C2
Plasmid size   186363 bp Coordinate of oriT [Strand]   39543..39693 [+]
Host baterium   Providencia stuartii strain FDAARGOS_645

Cargo genes

Drug resistance gene   qnrB4, floR, sul2, sul1, qacE, ant(3'')-Ia, blaOXA-10, cmlA1, aac(6')-IIc, ere(A)
Virulence gene   -
Metal resistance gene   merR, merT
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -