Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107943
Name   oriT_FDAARGOS_87|unnamed1 in_silico
Organism   Providencia stuartii strain FDAARGOS_87
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP031512 (17117..17267 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_FDAARGOS_87|unnamed1
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   5330 GenBank   WP_282555309
Name   mobH_MC79_RS20780_FDAARGOS_87|unnamed1 insolico UniProt ID   _
Length   1014 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1014 a.a.        Molecular weight: 112215.06 Da        Isoelectric Point: 5.0337

>WP_282555309.1 MULTISPECIES: MobH family relaxase [Providencia]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNETWQWVMTRSPTRCRGGPTMKKML

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4CP

ID   5598 GenBank   WP_000178856
Name   traD_MC79_RS20785_FDAARGOS_87|unnamed1 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69197.66 Da        Isoelectric Point: 6.9403

>WP_000178856.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.



ID   5599 GenBank   WP_000637386
Name   traC_MC79_RS20860_FDAARGOS_87|unnamed1 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92590.87 Da        Isoelectric Point: 5.9222

>WP_000637386.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 15201..40392

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MC79_RS20770 (MC79_020760) 11226..11459 - 234 WP_001191892 hypothetical protein -
MC79_RS20775 (MC79_020765) 11441..12058 - 618 WP_001249396 hypothetical protein -
MC79_RS20780 (MC79_020770) 12226..15204 + 2979 WP_001337757 MobH family relaxase -
MC79_RS20785 (MC79_020775) 15201..17066 + 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
MC79_RS23035 (MC79_020780) 17116..17661 + 546 WP_000228720 hypothetical protein -
MC79_RS20795 (MC79_020785) 17618..18247 + 630 WP_000743450 DUF4400 domain-containing protein tfc7
MC79_RS23040 18295..18471 + 177 WP_169342285 hypothetical protein -
MC79_RS20805 (MC79_020795) 18713..19090 + 378 WP_000869261 hypothetical protein -
MC79_RS20810 (MC79_020800) 19090..19752 + 663 WP_001231463 hypothetical protein -
MC79_RS21910 19933..20076 + 144 WP_001275801 hypothetical protein -
MC79_RS20815 (MC79_020805) 20088..20453 + 366 WP_001052531 hypothetical protein -
MC79_RS20820 (MC79_020810) 20598..20879 + 282 WP_000805625 type IV conjugative transfer system protein TraL -
MC79_RS20825 (MC79_020815) 20876..21502 + 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
MC79_RS20830 (MC79_020820) 21486..22403 + 918 WP_000794249 type-F conjugative transfer system secretin TraK -
MC79_RS20835 (MC79_020825) 22403..23716 + 1314 WP_024131605 TraB/VirB10 family protein -
MC79_RS20840 (MC79_020830) 23713..24291 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
MC79_RS20845 (MC79_020835) 24295..24687 + 393 WP_000479535 TraA family conjugative transfer protein -
MC79_RS20850 (MC79_020840) 24888..30419 + 5532 WP_036944418 Ig-like domain-containing protein -
MC79_RS20855 (MC79_020845) 30568..31275 + 708 WP_001259347 DsbC family protein -
MC79_RS20860 (MC79_020850) 31272..33719 + 2448 WP_000637386 type IV secretion system protein TraC virb4
MC79_RS20865 (MC79_020855) 33734..34051 + 318 WP_000351984 hypothetical protein -
MC79_RS20870 (MC79_020860) 34048..34578 + 531 WP_001010738 S26 family signal peptidase -
MC79_RS20875 (MC79_020865) 34541..35806 + 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein -
MC79_RS20880 (MC79_020870) 35803..36474 + 672 WP_001337754 EAL domain-containing protein -
MC79_RS20885 (MC79_020875) 36474..37478 + 1005 WP_043940352 TraU family protein -
MC79_RS20890 (MC79_020880) 37582..40392 + 2811 WP_080739811 conjugal transfer mating pair stabilization protein TraN -
MC79_RS20895 (MC79_020885) 40431..41267 - 837 WP_228715888 hypothetical protein -
MC79_RS20900 (MC79_020890) 41414..42055 - 642 WP_000796664 hypothetical protein -
MC79_RS20905 (MC79_020895) 42349..42669 + 321 WP_000547566 hypothetical protein -
MC79_RS20910 (MC79_020900) 42973..43158 + 186 WP_001186917 hypothetical protein -
MC79_RS20915 (MC79_020905) 43378..44346 + 969 WP_000085162 AAA family ATPase -
MC79_RS20920 (MC79_020910) 44357..45265 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   8378 GenBank   NZ_CP031512
Plasmid name   FDAARGOS_87|unnamed1 Incompatibility group   IncA/C2
Plasmid size   147900 bp Coordinate of oriT [Strand]   17117..17267 [+]
Host baterium   Providencia stuartii strain FDAARGOS_87

Cargo genes

Drug resistance gene   sul1, qacE, ant(3'')-Ia, dfrA14, sul2
Virulence gene   -
Metal resistance gene   merR, merT, merC
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -