Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107942
Name   oriT_pBML2537 in_silico
Organism   Providencia stuartii strain BML2537
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_AP022377 (50252..50402 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_pBML2537
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   5329 GenBank   WP_282555161
Name   mobH_G7Y71_RS20905_pBML2537 insolico UniProt ID   _
Length   1014 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1014 a.a.        Molecular weight: 112229.09 Da        Isoelectric Point: 5.0348

>WP_282555161.1 MobH family relaxase [Providencia stuartii]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNETWQWVMTRSPTRCRGGPTMKKML

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4CP

ID   5596 GenBank   WP_000178856
Name   traD_G7Y71_RS20910_pBML2537 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69197.66 Da        Isoelectric Point: 6.9403

>WP_000178856.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.



ID   5597 GenBank   WP_011872839
Name   traC_G7Y71_RS20990_pBML2537 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92576.85 Da        Isoelectric Point: 5.9216

>WP_011872839.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTDRIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 48336..73491

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G7Y71_RS20895 (BML2537_41720) 44361..44594 - 234 WP_001191892 hypothetical protein -
G7Y71_RS20900 (BML2537_41730) 44576..45193 - 618 WP_001249396 hypothetical protein -
G7Y71_RS20905 (BML2537_41740) 45361..48339 + 2979 WP_011872892 MobH family relaxase -
G7Y71_RS20910 (BML2537_41750) 48336..50201 + 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
G7Y71_RS22590 (BML2537_41760) 50251..50796 + 546 WP_000228720 hypothetical protein -
G7Y71_RS20920 (BML2537_41770) 50753..51382 + 630 WP_000743450 DUF4400 domain-containing protein tfc7
G7Y71_RS22595 51430..51606 + 177 WP_169342285 hypothetical protein -
G7Y71_RS20930 (BML2537_41780) 51848..52225 + 378 WP_164457051 hypothetical protein -
G7Y71_RS20935 (BML2537_41790) 52225..52887 + 663 WP_164457052 hypothetical protein -
G7Y71_RS20940 53067..53210 + 144 WP_164457053 hypothetical protein -
G7Y71_RS20945 (BML2537_41800) 53224..53589 + 366 WP_232074447 hypothetical protein -
G7Y71_RS20950 (BML2537_41810) 53734..54015 + 282 WP_000805625 type IV conjugative transfer system protein TraL -
G7Y71_RS20955 (BML2537_41820) 54012..54638 + 627 WP_025989284 TraE/TraK family type IV conjugative transfer system protein traE
G7Y71_RS20960 (BML2537_41830) 54622..55539 + 918 WP_025989283 type-F conjugative transfer system secretin TraK -
G7Y71_RS20965 (BML2537_41840) 55539..56852 + 1314 WP_024131605 TraB/VirB10 family protein -
G7Y71_RS20970 (BML2537_41850) 56849..57427 + 579 WP_164457054 type IV conjugative transfer system lipoprotein TraV traV
G7Y71_RS20975 (BML2537_41860) 57440..57823 + 384 WP_050584476 TraA family conjugative transfer protein -
G7Y71_RS20980 (BML2537_41870) 58024..63510 + 5487 WP_164457055 Ig-like domain-containing protein -
G7Y71_RS20985 (BML2537_41880) 63659..64366 + 708 WP_164457056 DsbC family protein -
G7Y71_RS20990 (BML2537_41890) 64363..66810 + 2448 WP_011872839 type IV secretion system protein TraC virb4
G7Y71_RS20995 (BML2537_41900) 66825..67142 + 318 WP_011872858 hypothetical protein -
G7Y71_RS21000 (BML2537_41910) 67139..67669 + 531 WP_021293639 S26 family signal peptidase -
G7Y71_RS21005 (BML2537_41920) 67632..68897 + 1266 WP_069142401 TrbC family F-type conjugative pilus assembly protein -
G7Y71_RS21010 (BML2537_41930) 68894..69565 + 672 WP_121926691 EAL domain-containing protein -
G7Y71_RS21015 (BML2537_41940) 69586..70569 + 984 WP_232074448 TraU family protein traU
G7Y71_RS21020 (BML2537_41950) 70687..73491 + 2805 WP_164457058 conjugal transfer mating pair stabilization protein TraN traN
G7Y71_RS21025 (BML2537_41960) 73530..74390 - 861 WP_164457059 hypothetical protein -
G7Y71_RS21030 (BML2537_41970) 74513..75163 - 651 WP_161647215 hypothetical protein -
G7Y71_RS21035 (BML2537_41980) 75456..75776 + 321 WP_000547566 hypothetical protein -
G7Y71_RS21040 (BML2537_41990) 76080..76265 + 186 WP_001186916 hypothetical protein -
G7Y71_RS21045 (BML2537_42000) 76485..77453 + 969 WP_164457060 AAA family ATPase -
G7Y71_RS21050 (BML2537_42010) 77464..78372 + 909 WP_164457061 hypothetical protein -

Host bacterium

ID   8377 GenBank   NZ_AP022377
Plasmid name   pBML2537 Incompatibility group   IncA/C2
Plasmid size   152754 bp Coordinate of oriT [Strand]   50252..50402 [+]
Host baterium   Providencia stuartii strain BML2537

Cargo genes

Drug resistance gene   sul1, qacE, aac(6')-Iae, blaIMP-10
Virulence gene   -
Metal resistance gene   merR, merT, merA
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -