Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 107912
• Name: oriT_FDAARGOS_919|unnamed1
• Organism: Bacillus cereus strain FDAARGOS_919
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP065879 (4160..4198 [-], 39 nt)
• oriT length: 39 nt
• IRs (inverted repeats): 1..6, 11..16 (CTTTAC..GTAAAG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 39 nt

>oriT_FDAARGOS_919|unnamed1
CTTTACGATAGTAAAGTATAGTGTGCTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_I6G76_RS00025_FDAARGOS_919|unnamed1

• ID: 5305
• GenBank: WP_000119308
• Name: mobV_I6G76_RS00025_FDAARGOS_919|unnamed1
• UniProt ID: Q4V103
• Length: 411 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 411 a.a.
• Molecular weight: 48609.55 Da
• Isoelectric Point: 9.5479

>WP_000119308.1 MobV family relaxase [Bacillus cereus]
MSYAICRMQKFKMRDVKGIQIHNQREKESHTNHDIESERTHLNYDLHNDGHIDYLKTVKDKIEQNVETNR
AIRKDAVVMCEFVVTSDKEFFDVLDMVDPVQQKVFFKEAYNFLKNRYGEQNIVHAAVHLDEKTPHMHVGM
VPVTEENKLSAKQIFNRKELVSLQDDFHAHMVEKGFYLERGVSSDKKHVEMRKLKAMTAKEEIKALEKEM
GQCLQKKEVVNKQMEQLESRLNQLQNAVKSTNKVDKMEVKHKGGILRSKTVEMSVEDFEELKTKAKASEA
FKRENMTLHRKNEALQASNTDLHTTVKRLEKENEGLKPYKARYERLAKLMDEMNKFYEKFIPKELSKFHE
IIGFCKRKVNASINRFSSLRYSEKELNENEKRGYETASKFLENQRKEQKQQRKERGDEREL

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-195)

Protein structure

• AlphaFold DB: Q4V103

Host bacterium

• ID: 8347
• GenBank: NZ_CP065879
• Plasmid name: FDAARGOS_919|unnamed1
• Incompatibility group: -
• Plasmid size: 5108 bp
• Coordinate of oriT [Strand]: 4160..4198 [-]
• Host baterium: Bacillus cereus strain FDAARGOS_919

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -