Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 107856
• Name: oriT_pDESACI.02
• Organism: Desulfosporosinus acidiphilus SJ4
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NC_018067 (91..121 [+], 31 nt)
• oriT length: 31 nt
• IRs (inverted repeats): 2..8, 12..18 (ACATTGC..GCAATGT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 31 nt

>oriT_pDESACI.02
CACATTGCGGAGCAATGTATAAGTGCGCCTT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobQ_DESACI_RS23345_pDESACI.02

• ID: 5271
• GenBank: WP_014825138
• Name: mobQ_DESACI_RS23345_pDESACI.02
• UniProt ID: I4DCV2
• Length: 561 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 561 a.a.
• Molecular weight: 65222.22 Da
• Isoelectric Point: 9.5531

>WP_014825138.1 MobQ family relaxase [Desulfosporosinus acidiphilus]
MAIYHLSSQIIGRSGGKSSVASAAYRSGEKIQDEHIGQTFDYTKKMGVDYKEILAPDHSPAWVQDRGQLW
NQVELAERRKDAQLARELNIALPKELSKEQQIELIRSFTKEKFVDKGMVADITMHHLDGENPHAHVMLTT
REIGPEGFGKKNREWNDRELLKDWRKEWAERTNQALEKADISERIDHRSLKDQGIKNRLPQKHIGVHASA
MEKRGIQTERGNQNRLILEINADLKSYDQDNAVYKQAEKQYQKAKSEADIHASKPTPVQPQPKPLGPEEK
EVSNEINTRDRNKAHEKPTPPIHEQTQPTTSFNQSFDEIIKGMETDGNTQSKALEPMEKEVGNGRNTLSV
DDWKKEILQAKQVTVQPQQQPQEKTYQEIRADFDQARIEFGRQQEAIDRLDSQLRSETFKLQKFDNYEKR
IGEVEERLGKLSSFKPWQWNEKKALEQSKARLIKDKQADFGETERGHIVQRITQLHSEKRSKEKKLPDLE
KRYKETKGTYDQRSAEIKAMNRQRDFVKSKDLLERYQTGENISDKDKQLINSFSKELNIKKIRIESLDRA
R

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (17-214)

Protein structure

• AlphaFold DB: I4DCV2

Host bacterium

• ID: 8291
• GenBank: NC_018067
• Plasmid name: pDESACI.02
• Incompatibility group: -
• Plasmid size: 3897 bp
• Coordinate of oriT [Strand]: 91..121 [+]
• Host baterium: Desulfosporosinus acidiphilus SJ4

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -