Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 107849 |
| Name | oriT_NRS6190_2  |
| Organism | Bacillus subtilis isolate NRS6190 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_OX419552 (11537..11898 [-], 362 nt) |
| oriT length | 362 nt |
| IRs (inverted repeats) | 291..296, 302..307 (AAAAAG..CTTTTT) 188..193, 198..203 (CACCAT..ATGGTG) 171..178, 180..187 (AAACCGCA..TGCGGTTT) 116..122, 130..136 (ACATAAA..TTTATGT) 84..89, 101..106 (ATTTAG..CTAAAT) 84..89, 92..97 (ATTTAG..CTAAAT) 62..67, 75..80 (CATAAA..TTTATG) 48..54, 65..71 (TTATTTT..AAAATAA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 362 nt
>oriT_NRS6190_2
AAAGAACAATCTCGTCATCGAAGACTAAATTTCTCTATGGAAAACAGTTATTTTTCGTGTGCATAAAATAAAGATTTATGTGCATTTAGTTCTAAATCACCTAAATAATGGTTGAACATAAATGTTTTTTTTATGTGCATTCAGCAATAAATCTGGTACCACGAAAAAACAAACCGCACTGCGGTTTCACCATGCAAATGGTGCCAGTTTCCCCTTATGCTCTTTCCTTTTCCACCCCCTCCTTCCTTCGGAATCGGGGGCCGGCTTTTTGCTGCCGCAAAAAGCCTTGGAAAAAGAAACACTTTTTTGGACAGATCGTTGCAAAGTAATGTGCAGAAATCGATGAAAAGGAGCGTTAACAA
AAAGAACAATCTCGTCATCGAAGACTAAATTTCTCTATGGAAAACAGTTATTTTTCGTGTGCATAAAATAAAGATTTATGTGCATTTAGTTCTAAATCACCTAAATAATGGTTGAACATAAATGTTTTTTTTATGTGCATTCAGCAATAAATCTGGTACCACGAAAAAACAAACCGCACTGCGGTTTCACCATGCAAATGGTGCCAGTTTCCCCTTATGCTCTTTCCTTTTCCACCCCCTCCTTCCTTCGGAATCGGGGGCCGGCTTTTTGCTGCCGCAAAAAGCCTTGGAAAAAGAAACACTTTTTTGGACAGATCGTTGCAAAGTAATGTGCAGAAATCGATGAAAAGGAGCGTTAACAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 5269 | GenBank | WP_282326770 |
| Name | mobP2_KJP53_RS21365_NRS6190_2 |
UniProt ID | _ |
| Length | 410 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 410 a.a. Molecular weight: 48499.03 Da Isoelectric Point: 10.0640
>WP_282326770.1 MobP2 family relaxase [Bacillus subtilis]
MGSPGVVLVSKYVSGKSTKFSKYVNYINRDEAVRTEKFQTYNVNKLDGYNQYMGNPEKSSGIFTQHKDSL
SPKEKNQLKEIFRQAQKNDSVMWQDVISFDNKWLEERGIYNSKTGWVNEGAIQNSIRKGMEVLLREEQLE
QSGVWSAAIHYNTDNIHVHIALVEPNPTKEYGVFTNKKTGEVYQARRGNRKLKTLDKMKSKVANTLMDRD
KELSKISQLIHDRIAPKGLKFQPRLDTYMTKMYNHIYENLPEDMRLWKYNNNALNNIRPEIDSVITMYIQ
KYHPEDYKELDQSLKEEMEFRKSVYGDGPKQVERYKEYRKNKYKELYTKLGNSMLKEMAEIRRSESQSKQ
INRSGTYAPGSVSHRQWDTKGRRLKRSDINKIKHALDKDYQSMKNMRKYQQMQYEMEQSR
MGSPGVVLVSKYVSGKSTKFSKYVNYINRDEAVRTEKFQTYNVNKLDGYNQYMGNPEKSSGIFTQHKDSL
SPKEKNQLKEIFRQAQKNDSVMWQDVISFDNKWLEERGIYNSKTGWVNEGAIQNSIRKGMEVLLREEQLE
QSGVWSAAIHYNTDNIHVHIALVEPNPTKEYGVFTNKKTGEVYQARRGNRKLKTLDKMKSKVANTLMDRD
KELSKISQLIHDRIAPKGLKFQPRLDTYMTKMYNHIYENLPEDMRLWKYNNNALNNIRPEIDSVITMYIQ
KYHPEDYKELDQSLKEEMEFRKSVYGDGPKQVERYKEYRKNKYKELYTKLGNSMLKEMAEIRRSESQSKQ
INRSGTYAPGSVSHRQWDTKGRRLKRSDINKIKHALDKDYQSMKNMRKYQQMQYEMEQSR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 5515 | GenBank | WP_282326796 |
| Name | t4cp2_KJP53_RS21415_NRS6190_2 |
UniProt ID | _ |
| Length | 786 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 786 a.a. Molecular weight: 90588.06 Da Isoelectric Point: 5.0287
>WP_282326796.1 VirD4-like conjugal transfer protein, CD1115 family [Bacillus subtilis]
MHKEFKQWKKVFADKYFLIAFTLFCYVAVTCITNFFIHLLKQIPVILSSFKNFDQSQLSSPFSSMTWKWF
FKFDWSMGIVYGVVYVLASIFILRQVYRFRIAFRDINKQTKGTARWTEIKEIQETYKAVKDDDNEYEGSA
GMPIVHFNDHLYVDTNSTHTLVVASTQSGKTETYSYPYLDVIMRAKEKDSVVITDIKGDMLKNTRAEFEK
YGYEVMCFNLLNPYWGMAYNPLELVKQAYLKKDYSKAQMLCNTLSYSLFHNDKSHADPMWENASIALVNA
LILAVCDLCIKNGQPENITMYTLTVMLNELGSNPDEDGYTRLDHFFGNLPPSHPAKLQYGTIQFSQGITR
SGIYTGTMAKLKNYTYDTIARMTARNDLNIEDLAYGEKPVALFIVYPDWDDSNYTIISTFLSQVNAVLSE
KATLSKESTLPRKVRFLFEEVANIPPIEGLNRSLAVGLSRGMLYTLVIQNVSQLRDVYGDDMAVAIMGNL
GNQIYIMSDEWEDAEKFSEKLGVTTVISADRQGDLMDVHKSYSEREEERPLMLPDELRRLKKGEWVVLRT
KKREDLKRNRVVPYPIFASLDNGTNMLHRYEYLMHRFDNKIALGDLGFRGEHETLDLESLLIEFEFNEPV
KEERKSKPGKGKKQKAKKAPDEPVSSELPLPEEPIGFTESSEGMDSLFASIPVENTNEHGFYEDPPMNES
SHDDDLEASEEDIIMNSLEEVYETSTPISDAIKPDHYVYIKLLAHKQLSESEYAYFESLKTVEELRAFFR
APEKKEIYEQIKTHIE
MHKEFKQWKKVFADKYFLIAFTLFCYVAVTCITNFFIHLLKQIPVILSSFKNFDQSQLSSPFSSMTWKWF
FKFDWSMGIVYGVVYVLASIFILRQVYRFRIAFRDINKQTKGTARWTEIKEIQETYKAVKDDDNEYEGSA
GMPIVHFNDHLYVDTNSTHTLVVASTQSGKTETYSYPYLDVIMRAKEKDSVVITDIKGDMLKNTRAEFEK
YGYEVMCFNLLNPYWGMAYNPLELVKQAYLKKDYSKAQMLCNTLSYSLFHNDKSHADPMWENASIALVNA
LILAVCDLCIKNGQPENITMYTLTVMLNELGSNPDEDGYTRLDHFFGNLPPSHPAKLQYGTIQFSQGITR
SGIYTGTMAKLKNYTYDTIARMTARNDLNIEDLAYGEKPVALFIVYPDWDDSNYTIISTFLSQVNAVLSE
KATLSKESTLPRKVRFLFEEVANIPPIEGLNRSLAVGLSRGMLYTLVIQNVSQLRDVYGDDMAVAIMGNL
GNQIYIMSDEWEDAEKFSEKLGVTTVISADRQGDLMDVHKSYSEREEERPLMLPDELRRLKKGEWVVLRT
KKREDLKRNRVVPYPIFASLDNGTNMLHRYEYLMHRFDNKIALGDLGFRGEHETLDLESLLIEFEFNEPV
KEERKSKPGKGKKQKAKKAPDEPVSSELPLPEEPIGFTESSEGMDSLFASIPVENTNEHGFYEDPPMNES
SHDDDLEASEEDIIMNSLEEVYETSTPISDAIKPDHYVYIKLLAHKQLSESEYAYFESLKTVEELRAFFR
APEKKEIYEQIKTHIE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Host bacterium
| ID | 8284 | GenBank | NZ_OX419552 |
| Plasmid name | NRS6190_2 | Incompatibility group | - |
| Plasmid size | 64136 bp | Coordinate of oriT [Strand] | 11537..11898 [-] |
| Host baterium | Bacillus subtilis isolate NRS6190 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |