Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   107839
Name   oriT_pJD053-MCR-59K in_silico
Organism   Escherichia fergusonii strain EF21JD053
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP095792 (11931..11983 [-], 53 nt)
oriT length   53 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 53 nt

>oriT_pJD053-MCR-59K
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


T4CP


ID   5507 GenBank   WP_015059539
Name   t4cp2_MYB88_RS25330_pJD053-MCR-59K insolico UniProt ID   _
Length   652 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 652 a.a.        Molecular weight: 73404.02 Da        Isoelectric Point: 9.4339

>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE

  Protein domains


Predicted by InterproScan.

(127-591)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 30844..54301

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYB88_RS25270 (MYB88_25265) 26333..27457 - 1125 WP_000486716 site-specific integrase -
MYB88_RS25275 (MYB88_25270) 27553..27750 + 198 WP_001356717 hypothetical protein -
MYB88_RS25630 28598..28819 + 222 Protein_31 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MYB88_RS25280 (MYB88_25275) 28816..30192 - 1377 WP_000750519 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MYB88_RS25285 (MYB88_25280) 30205..30840 - 636 WP_000934977 A24 family peptidase -
MYB88_RS25290 (MYB88_25285) 30844..31326 - 483 WP_001258095 lytic transglycosylase domain-containing protein virB1
MYB88_RS25295 (MYB88_25290) 31392..31949 - 558 WP_000095048 type 4 pilus major pilin -
MYB88_RS25300 (MYB88_25295) 31994..33103 - 1110 WP_000974903 type II secretion system F family protein -
MYB88_RS25305 (MYB88_25300) 33094..34632 - 1539 WP_000466225 ATPase, T2SS/T4P/T4SS family virB11
MYB88_RS25310 (MYB88_25305) 34657..35151 - 495 WP_000912553 type IV pilus biogenesis protein PilP -
MYB88_RS25315 (MYB88_25310) 35135..36445 - 1311 WP_001454111 type 4b pilus protein PilO2 -
MYB88_RS25320 (MYB88_25315) 36496..38139 - 1644 WP_001035592 PilN family type IVB pilus formation outer membrane protein -
MYB88_RS25325 (MYB88_25320) 38132..38668 - 537 WP_001220543 sigma 54-interacting transcriptional regulator virb4
MYB88_RS25330 (MYB88_25325) 38715..40673 - 1959 WP_015059539 type IV secretory system conjugative DNA transfer family protein -
MYB88_RS25335 (MYB88_25330) 40689..41744 - 1056 WP_001059977 P-type DNA transfer ATPase VirB11 virB11
MYB88_RS25340 (MYB88_25335) 41763..42902 - 1140 WP_000790640 TrbI/VirB10 family protein virB10
MYB88_RS25345 (MYB88_25340) 42892..43593 - 702 WP_000274524 TrbG/VirB9 family P-type conjugative transfer protein -
MYB88_RS25350 (MYB88_25345) 43659..44393 - 735 WP_000432282 type IV secretion system protein virB8
MYB88_RS25355 (MYB88_25350) 44559..46916 - 2358 WP_063078682 VirB4 family type IV secretion system protein virb4
MYB88_RS25360 (MYB88_25355) 46922..47242 - 321 WP_000362080 VirB3 family type IV secretion system protein virB3
MYB88_RS25365 (MYB88_25360) 47313..47603 - 291 WP_000865479 conjugal transfer protein -
MYB88_RS25370 (MYB88_25365) 47603..48187 - 585 WP_001177113 lytic transglycosylase domain-containing protein virB1
MYB88_RS25375 (MYB88_25370) 48208..48606 - 399 WP_001153665 hypothetical protein -
MYB88_RS25380 (MYB88_25375) 48725..49162 - 438 WP_000539665 type IV pilus biogenesis protein PilM -
MYB88_RS25385 (MYB88_25380) 49168..50403 - 1236 WP_015059538 TcpQ domain-containing protein -
MYB88_RS25390 (MYB88_25385) 50406..50705 - 300 WP_000835764 TrbM/KikA/MpfK family conjugal transfer protein -
MYB88_RS25395 (MYB88_25390) 50773..51054 - 282 WP_000638823 type II toxin-antitoxin system RelE/ParE family toxin -
MYB88_RS25400 (MYB88_25395) 51044..51295 - 252 WP_000121741 hypothetical protein -
MYB88_RS25405 (MYB88_25400) 51395..52030 - 636 WP_015059536 hypothetical protein -
MYB88_RS25410 (MYB88_25405) 52103..52390 - 288 WP_001032611 EexN family lipoprotein -
MYB88_RS25415 (MYB88_25410) 52403..52657 - 255 WP_001043555 EexN family lipoprotein -
MYB88_RS25420 (MYB88_25415) 52659..53300 - 642 WP_001425343 type IV secretion system protein -
MYB88_RS25425 (MYB88_25420) 53306..54301 - 996 WP_001028543 type IV secretion system protein virB6
MYB88_RS25430 (MYB88_25425) 54305..54562 - 258 WP_000739144 hypothetical protein -
MYB88_RS25435 (MYB88_25430) 54559..54861 - 303 WP_001360345 hypothetical protein -
MYB88_RS25440 (MYB88_25435) 54842..55099 - 258 WP_001542015 hypothetical protein -
MYB88_RS25445 (MYB88_25440) 55132..55578 - 447 WP_001243165 hypothetical protein -
MYB88_RS25450 (MYB88_25445) 55589..55759 - 171 WP_000550720 hypothetical protein -
MYB88_RS25455 (MYB88_25450) 55763..56206 - 444 WP_000964330 NfeD family protein -
MYB88_RS25460 (MYB88_25455) 56580..57533 - 954 WP_072089442 SPFH domain-containing protein -
MYB88_RS25465 (MYB88_25460) 57560..57736 - 177 WP_000753050 hypothetical protein -
MYB88_RS25470 (MYB88_25465) 57729..57944 - 216 WP_001127357 DUF1187 family protein -
MYB88_RS25475 (MYB88_25470) 57937..58389 - 453 WP_000101552 CaiF/GrlA family transcriptional regulator -


Host bacterium


ID   8274 GenBank   NZ_CP095792
Plasmid name   pJD053-MCR-59K Incompatibility group   IncI2
Plasmid size   59528 bp Coordinate of oriT [Strand]   11931..11983 [-]
Host baterium   Escherichia fergusonii strain EF21JD053

Cargo genes


Drug resistance gene   mcr-1.1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -