Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107827
Name   oriT_FAM24678|unnamed1 in_silico
Organism   Morganella morganii strain FAM24678
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP066131 (6977..7012 [-], 36 nt)
oriT length   36 nt
IRs (inverted repeats)      9..16, 18..25  (GCAAATTT..AAATTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 36 nt

>oriT_FAM24678|unnamed1
AAATTCATGCAAATTTTAAATTTGCGTAGTGTGTGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   5253 GenBank   WP_096862173
Name   mobF_JC792_RS20000_FAM24678|unnamed1 insolico UniProt ID   _
Length   1693 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1693 a.a.        Molecular weight: 184635.03 Da        Isoelectric Point: 6.9602

>WP_096862173.1 MobF family relaxase [Morganella morganii]
MLSVAPIAQNAENYYTAQDNYYFLGNAESRWLGKGAEALGLTGEVSKEQLREILAGRLPEGSSLERTENG
KNTHRQGHDLTFSAPKSVSVLAVVLGDERMLAAHNAAVTAALKETETLASTRVMKDGISAPVMTGNLVIA
AFNHDTSRELDPQLHTHALVMNMTAHEGQWRTLSSDTRHNSGFSEAVYKTRISLGRIYLHELRQQVEKMG
FKTHDTGRNGLWEIEGIPVAPFSQRRQQILDAAGHDASLKSRDVAALDTRQAKRTPDKAELLTEWYDRLD
KNGFGSEERSRFNTAAQSREQDSLTPPPLPAALQKAADAALTEAVNALSERQVSMTYSAVLAKTLSGLDA
RPGIITVVRAAIDRAIEQRQLIPLDEQKGLFTSAAHLTDELTLQQLAAEVRTENRTPVFRVPVTPFTGTA
QKLADTLPNAAVIDSRGNRQMQRDRLAETAAVALLQGRTVLVIEADSKHTPPPVSGTLSTDKRRWTEKPA
LPVNGTVIVTGAETWSLKETVQLFAQAKETNTQILLLDSAGRKGTGNALRTLEDAGVVRFTAAGKPPVQT
EIHSIGDKQQRYAAVAARYQALRREGVPVTVQVTGTRERAALTDAVRAELTAQGVLQGSPVSVTLLTPVW
SDSKTRRDISQYREGMILEQWQDGKKSAERFTVSRVTPATRSVTLEDSRGQSQVIKVTKLDSSWSLFHPA
QKAVAAGETLTLLAKHGRMAAGDTLTVTAVTDSILTAEHRGRRVTLPLTAGLKADYAYVTTPGQSLPDNQ
VVLAAASVRDTRADFFNTVARSGEKIELFTPLDNTETDRRLAKSPHYQTVAARLGTDPQNPDNSVTQAAE
RLWSVPEKAVRQGIVLTQENQVMFSVTDVVAKTLPLHPSLTAESIRLQTAKMAAAGELIPVPGSGKNEYV
TAAAWESEKQIIRAILQGKGTQQPFFERVPEAMLTGLTPGQQAATRLILESTDRFIAVQGYAGVGKTTQF
KAVMRSLDLLPEDARPEVRGLAPTHRAVGEMAATGVKAQTLQSFLTDTAQQLRNGETPRFDNTLFLIDES
SMIGNRNMAEVVAVIEAGGGRAVKSGDTAQEQAIDSGTPFELAQTRSALDTAIMKEIVRQTPALKPAVEA
VIAGQMKQAAALLENVSPDIVPRLPDAHVPEKSVMQAGEEGVIRDIVRDYSSRTPEAREKTLIVVQTNAD
RAAVNAGVHEALTAGQDVRRIRIPVLVQEKTQTESLHSVAGLAQHHGKTVLAGDNYYTLIVTERDKADGV
VMLRDADKREFPLSAFENSSRDIQVFRTETREMAEGEKVSFTRTDRNRGRVVNSTRTVSQIHDDGTLTLT
DGRDSIRINPAEPADRHIDYGYAGTAHKAQGASEEYVIVLGGVTGGRQPLASRRNAYVALSRMKTHVQVY
SDDLDKWLDKTGSNAVRPTVHDLLLADSDRAAATGNRLYAGAKPLSDTAVGRTLSRELGLTAESTARFVY
GSQKYPEPGVAWPVYDRHGRPAGTRIMPVLLNEQGHLNGAGQEHRLVGREEAAWMVLQRGRNGETRIAES
MTEALAQLKQTPESGVVVRLQPEEPVNAAVMQKLTGGLPADLSVPDTLRAGVPAADDPMTLKTAEEQRRE
AELKAAALPSKTDTVRDAPQDARLEAEITREQQRLQSQENAHEQKAAISLDDMIRQEQLHRMRQTEQLQQ
AEKDIVREKEIGE

  Protein domains


Predicted by InterproScan.

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(626-700)

AAA domain (AAA_30)

(947-1134)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(567-616)

TrwC relaxase (TrwC)

(8-280)

DNA helicase TraI (TraI_C)

(1388-1502)


  Protein structure



No available structure.




T4CP

ID   5499 GenBank   WP_096862174
Name   traD_JC792_RS19995_FAM24678|unnamed1 insolico UniProt ID   _
Length   735 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 735 a.a.        Molecular weight: 83586.48 Da        Isoelectric Point: 5.2300

>WP_096862174.1 type IV conjugative transfer system coupling protein TraD [Morganella morganii]
MSSTKHITQGGQVFGYMLGMFMQVNKRISFYLILFYLILTPVIFYLKVPLQEIKNGGLYWWLYFMSPGEK
ALYRVPPVYDFNFDGTVYHFTSANILKDPYMTYAGDIFLQELWLSLFWSSVLVLLLAFVVYSLLHRLGEK
QARDETTGGRELTEDVSAVARQMQRRREASPIRIDKLPLILNSEVKNMLMHGTPGSGKSNTINKLLLQLR
ERGDMVIIFDKGCSLVKKHFNENCDKLLNPLDARCENWDLWRECLTTPDFDSMSNTLIPQGTKEDPFWTG
SARTIFTAVAAKLRNDPERSYNKLLRTLLAIDLKALREYVAGTEASNLMEEKVEKTAISIRGVLTNYVKA
LRYLQGIERNGKAPFAIREWMKQANEPKKPHGWLWITSNARQHESLKPLISMWLAQAANCVLEMNENPER
RIWFIYDELASLHKLPELPQVLSEARKFGGCFVLGFQNKPQLDFTYGEDYADAMMDLLNTRFFFRSPDQN
VAMWVRDQLGQKRAKLFNEQYSYGADTVRDGVSFTKQEDDKTLVSYSDIQKLPDLECYVTLPGDYPVVRM
KMTYEKIRPVAAEFIERELNDSLEQDITARLDAGEQDDARLQRLLSSSTPAPETPSGLPPQTAAVLVVPP
VLTMAAAHPAPVVPSVTTPVTAVSSENTTQTAQTDTAVTGGREQDVPDMVIDTATGEIVYPGADEPDAET
RAVMYENYSALHQEEKNIAVHRRAEPDDHDEPEVW

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(33-130)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(173-562)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 9524..32262

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JC792_RS19840 (JC792_19835) 5221..6165 + 945 WP_218453978 zincin-like metallopeptidase domain-containing protein -
JC792_RS19845 (JC792_19840) 6447..6761 - 315 WP_348773190 lytic transglycosylase domain-containing protein -
JC792_RS19850 (JC792_19845) 7320..7703 + 384 WP_218453979 relaxosome protein TraM -
JC792_RS19855 (JC792_19850) 7906..8625 + 720 WP_218453980 hypothetical protein -
JC792_RS19860 (JC792_19855) 8677..8871 + 195 WP_218453981 TraY domain-containing protein -
JC792_RS19865 (JC792_19860) 9118..9495 + 378 WP_172694096 type IV conjugative transfer system pilin TraA -
JC792_RS19870 (JC792_19865) 9524..9829 + 306 WP_172694066 type IV conjugative transfer system protein TraL traL
JC792_RS19875 (JC792_19870) 9840..10412 + 573 WP_218453982 type IV conjugative transfer system protein TraE traE
JC792_RS19880 (JC792_19875) 10393..11166 + 774 WP_247717512 type-F conjugative transfer system secretin TraK traK
JC792_RS19885 (JC792_19880) 11168..12550 + 1383 WP_218453983 TrbI/VirB10 family protein traB
JC792_RS19890 (JC792_19885) 12566..12790 + 225 WP_172694063 hypothetical protein -
JC792_RS19895 (JC792_19890) 12792..12956 + 165 WP_172694062 hypothetical protein -
JC792_RS19900 (JC792_19895) 13113..13292 + 180 WP_218453984 hypothetical protein -
JC792_RS19905 (JC792_19900) 13303..13629 + 327 WP_218453985 hypothetical protein -
JC792_RS19910 (JC792_19905) 13754..14014 + 261 WP_218453986 hypothetical protein -
JC792_RS19915 (JC792_19910) 14019..14540 + 522 WP_172694058 type IV conjugative transfer system lipoprotein TraV traV
JC792_RS19920 (JC792_19915) 14540..17248 + 2709 WP_247717513 type IV secretion system protein TraC virb4
JC792_RS20575 17224..17625 + 402 Protein_23 type-F conjugative transfer system protein TrbI -
JC792_RS20580 17653..18279 + 627 WP_172694056 type-F conjugative transfer system protein TraW traW
JC792_RS19930 (JC792_19925) 18291..19259 + 969 WP_247715250 conjugal transfer pilus assembly protein TraU traU
JC792_RS19935 (JC792_19930) 19271..19882 + 612 WP_247717514 type-F conjugative transfer system pilin assembly protein TrbC trbC
JC792_RS19940 (JC792_19935) 19882..21699 + 1818 WP_218453988 type-F conjugative transfer system mating-pair stabilization protein TraN traN
JC792_RS19945 (JC792_19940) 21696..22475 + 780 WP_218453989 type-F conjugative transfer system pilin assembly protein TraF traF
JC792_RS19950 (JC792_19945) 22491..22775 + 285 WP_193830285 hypothetical protein -
JC792_RS19955 (JC792_19950) 22772..23296 + 525 WP_218453990 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
JC792_RS19960 (JC792_19955) 23390..23746 + 357 WP_130184729 hypothetical protein -
JC792_RS19965 (JC792_19960) 23739..25136 + 1398 WP_218453991 conjugal transfer pilus assembly protein TraH traH
JC792_RS19970 (JC792_19965) 25126..27993 + 2868 WP_218453992 conjugal transfer mating-pair stabilization protein TraG traG
JC792_RS19975 (JC792_19970) 28050..28373 + 324 WP_218453993 YbjQ family protein -
JC792_RS19980 (JC792_19975) 28415..29152 + 738 WP_218453994 hypothetical protein -
JC792_RS19985 (JC792_19980) 29246..29452 + 207 WP_096862176 TraR/DksA C4-type zinc finger protein -
JC792_RS19990 (JC792_19985) 29516..29893 + 378 WP_096862175 hypothetical protein -
JC792_RS19995 (JC792_19990) 30055..32262 + 2208 WP_096862174 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   8262 GenBank   NZ_CP066131
Plasmid name   FAM24678|unnamed1 Incompatibility group   -
Plasmid size   72755 bp Coordinate of oriT [Strand]   6977..7012 [-]
Host baterium   Morganella morganii strain FAM24678

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -