Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107825
Name   oriT_p621164-FII in_silico
Organism   Morganella morganii strain 1606-21164
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT701512 (74..109 [+], 36 nt)
oriT length   36 nt
IRs (inverted repeats)      9..16, 18..25  (GCAAATTT..AAATTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 36 nt

>oriT_p621164-FII
AAATTCATGCAAATTTTAAATTTGCGTAGGGTGTGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   5251 GenBank   WP_214183221
Name   traI_MTW10_RS00140_p621164-FII insolico UniProt ID   _
Length   1699 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1699 a.a.        Molecular weight: 185169.63 Da        Isoelectric Point: 6.8458

>WP_214183221.1 conjugative transfer relaxase/helicase TraI [Morganella morganii]
MLSVAPIAKNAENYYTAQDNYYFLGNAESRWMGKGAEALGLTGEVSKEQLQEILAGRLPEGSSLERTENG
KNTHRQGHDLTFSAPKSVSVLAVVLGDERMLAAHNAAVTAALNETETLASTRVMKDGASAPAMTGNLVIA
AFNHDTSRELDPQLHTHALVMNMTAHEGQWRTLSSDTRHKSGFTEAVYASQVALGQIYRHELRRQVESLG
YRTQETGKNGLWEIAGVPVAPFSLRRQQILDAAGHDASLKSLDVAALDTRQAKRTPDKAELLTEWYDRLD
KNGFGSEERSRFNTAAQSREQDSLTPPPLPAALQKAADTALTEAVNALSERQVSMTYSAVLAKTLSGLDA
RPGIITVVRNAIDRAIEQRHLIPLDEQKGLFTSAAHLTDELTLQQLAAEVRTENRTPVFRVPETVFTGTA
QKLADTLPNAAVIDSRGNRQMQRDRLAETAAVALQQGRTVLVIEADSKHTPEPVSGTLSTDKRRWTEKPA
LPVNGTVIVTGAETWSLKETVQLFAQAKETNTQILLLDSAGRKGTGNALRTLEDAGVVRFAAAGKPPVQT
EIHSIGDKQQRYAAVAARYQALRREGVPVTVQVMGTRERAALTDAVRAELTAQGVLQGSPVSVTLLTPVW
SDSKTRRDISQYREGMVLEQWQDGKKSAERFTVTRVTPATRSVTLEDNRGQSQVIKVTKLDSSWSLFHTS
QKAIAAGETLILLAKHGRMAAGDTLTVTAVTDSTLTAEHHGRRVTLPLTAGLKADYVYVTTPGQSLPDNQ
VVLAAASVRDTRADFFNTVARSGEKVELFTPLDNTETDLRLARSPHYQTVSAQLGTDPQNPDTSVRQAAE
RLWSVPEKAVRQGIVLTQENQVMFSVTDVVAKTLPLHPSLTAESIRLQTAKMAAAGELIPVPESGKNEYV
TAAAWESEKQIIRAILKGKGTQQPFFERVPEAVLAGLTPGQQAATRLILESTDRFIAVQGYAGVGKTTQF
KAVMRSLDLLPEDARPEVRGLAPTHRAVGEMAATGVKAQTLQSFLTDTAQLLRNGETPRFDNTLFLIDES
SMIGNRNMAEVVAVIEAGGGRAVKSGDTAQEQAIDSGTPFELAQTRSALDTAIMKEIVRQTPALKPAVEA
VIAGQMKQAAALLENVSPDIVPRLPDAHVPEKSVMQAGEEGVIRDIVRDYSSRTPEAREKTLIVVQTNAD
RAAVNTGVHEALTAGQDVRRVRMPVLVQEKTQTESLHSVAGLAQHHGKTVLAGDTYYTLIVTERDKADGV
VMLRDADRRDIPLSAFENSSRDIQVFRTETREIAEGEKVTFTRTDRNRGRVVNSTRTVSQIHDDGTLTLT
DGRDSIRINPAEPADRHIDYGYAGTAHKAQGASEEYVIVLGGVTGGRQPLASRRNAYVALSRMKTHVQVY
SDNLEKWLDKTGSNAARPTVHDLLLAESDRAAATGNRLYSGAKPLADTAVGRALSRELGLTAESTARFVY
GSQKYPEPGVAWPVYDRHGRPAGTRITPVLLNEQGQLNGLGQEHRLVGREEAAWIVLQRGSSGETRIAES
MTEALAQLKQTPDSGVVVRLQPEEPVNAAVMRKLTGGLPADLSVPDTRRAGVPAADDPMTLKTAEEQRRE
AELKAAVLPEKADTVRDALPDARLDAEITREQQRLQLQENAHDRKAAVSLDEILRQEQLHRMRQTEKLQQ
AGKDIVREKEIVREKEIGE

  Protein domains


Predicted by InterproScan.

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(626-700)

TrwC relaxase (TrwC)

(8-280)

DNA helicase TraI (TraI_C)

(1388-1503)

AAA domain (AAA_30)

(947-1134)


  Protein structure



No available structure.




T4CP

ID   5498 GenBank   WP_214183222
Name   traD_MTW10_RS00145_p621164-FII insolico UniProt ID   _
Length   740 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 740 a.a.        Molecular weight: 84056.94 Da        Isoelectric Point: 5.3118

>WP_214183222.1 type IV conjugative transfer system coupling protein TraD [Morganella morganii]
MSSTKHITQGGQVFGYMLGMFMQVNKRISFYLILFYLILTPVIFYLKVPLQEIKNGGLYWWLYFMSPGEK
ALYRVPPVYDISFDGTVYYFTAENILKDPYMTYAGDIFLQELWLSLFWASVLVLLLAFVVYTLLHRLGEK
QARDETTGGRELTEDVSAVARQMQRRREASPIRIDKLPLILNSEVKNMLMHGTPGSGKSNTINKLLRQLR
ERGDMVIIFDKGCSLVKKHFNENRDKLLNPLDARCENWDLWRECLTTPDFDSMSNTLIPQGTKEDPFWTG
SARTIFTAVAAKLRNDPERSYNRLLRTLLAIDLKALREYVAGTEASNLMEEKVEKTAISIRGVLTNYVKA
LRYLQGIERNGKAPFAIREWMRQANEPKKPHGWLWITSNARQHESLKPLISMWLAQAANCVLEMNENPER
RIWFIYDELASLHKLPELPQVLSEARKFGGCFVLGFQNKPQLDFTYGEDYADAMMDLLNTRFFFRSPDQN
VAMWVRDQLGQKRAKLFNEQYSYGADTVRDGVSFSKQEDDKTLVSYSDIQKLPDLECYVTLPGDYPVVRM
KMTYEKIRPVAAEFIERELNDSLEQDITAHLDAGRQDDARLQRLLDGSGTPAPETPSGLPPQTAAVLVVP
PVLTPAAAHPAPVVSAVVPSVTTPAAAVTAENTTQTTQTDTAVTGGREQDVPDMVIDTATGEIVYPGADE
PDAETRAAMYENYSALHQEEKNIAVHRRAEPDDHDEPEVW

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(33-129)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(173-562)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 29991..51972

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MTW10_RS00145 29991..32213 - 2223 WP_214183222 type IV conjugative transfer system coupling protein TraD virb4
MTW10_RS00150 32372..32578 - 207 WP_214183223 TraR/DksA C4-type zinc finger protein -
MTW10_RS00155 32662..33075 - 414 WP_214183224 hypothetical protein -
MTW10_RS00160 33080..35932 - 2853 WP_214183225 conjugal transfer mating-pair stabilization protein TraG traG
MTW10_RS00165 35922..37319 - 1398 WP_214183226 conjugal transfer pilus assembly protein TraH traH
MTW10_RS00170 37312..37671 - 360 WP_214183227 hypothetical protein -
MTW10_RS00175 37764..38288 - 525 WP_214183228 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
MTW10_RS00180 38285..38569 - 285 WP_214183229 hypothetical protein -
MTW10_RS00185 38585..39376 - 792 WP_214183230 type-F conjugative transfer system pilin assembly protein TraF traF
MTW10_RS00190 39373..41190 - 1818 WP_214183231 type-F conjugative transfer system mating-pair stabilization protein TraN traN
MTW10_RS00195 41183..41800 - 618 WP_242645381 type-F conjugative transfer system pilin assembly protein TrbC trbC
MTW10_RS00200 41812..42780 - 969 WP_242645392 conjugal transfer pilus assembly protein TraU traU
MTW10_RS00205 42792..43421 - 630 WP_214183233 type-F conjugative transfer system protein TraW traW
MTW10_RS00210 43418..43849 - 432 WP_214183234 type-F conjugative transfer system protein TrbI -
MTW10_RS00215 43825..46533 - 2709 WP_242645382 type IV secretion system protein TraC virb4
MTW10_RS00220 46533..47054 - 522 WP_214183235 type IV conjugative transfer system lipoprotein TraV traV
MTW10_RS00225 47078..47446 - 369 WP_214183236 hypothetical protein -
MTW10_RS00230 47459..47716 - 258 WP_242645383 hypothetical protein -
MTW10_RS00235 47859..48212 - 354 WP_242645384 hypothetical protein -
MTW10_RS00240 48252..48761 - 510 WP_242645385 hypothetical protein -
MTW10_RS00245 48771..48935 - 165 WP_214183240 hypothetical protein -
MTW10_RS00250 48946..50328 - 1383 WP_214183241 TrbI/VirB10 family protein traB
MTW10_RS00255 50330..51103 - 774 WP_242645386 type-F conjugative transfer system secretin TraK traK
MTW10_RS00260 51084..51656 - 573 WP_214183242 type IV conjugative transfer system protein TraE traE
MTW10_RS00265 51667..51972 - 306 WP_046894769 type IV conjugative transfer system protein TraL traL
MTW10_RS00270 52001..52378 - 378 WP_214183243 type IV conjugative transfer system pilin TraA -
MTW10_RS00275 52630..52821 - 192 WP_214183244 TraY domain-containing protein -
MTW10_RS00280 52911..53576 - 666 WP_214183245 hypothetical protein -
MTW10_RS00285 53778..54242 - 465 WP_242645387 relaxosome protein TraM -

Host bacterium

ID   8260 GenBank   NZ_MT701512
Plasmid name   p621164-FII Incompatibility group   -
Plasmid size   54395 bp Coordinate of oriT [Strand]   74..109 [+]
Host baterium   Morganella morganii strain 1606-21164

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -