Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107810
Name   oriT_AR_0133|unnamed2 in_silico
Organism   Morganella morganii strain AR_0133
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP028958 (69616..69716 [+], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      80..85, 91..96  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_AR_0133|unnamed2
TATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   5244 GenBank   WP_012561166
Name   mobF_AM380_RS21145_AR_0133|unnamed2 insolico UniProt ID   D7RU03
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120141.30 Da        Isoelectric Point: 6.5229

>WP_012561166.1 MULTISPECIES: MobF family relaxase [Gammaproteobacteria]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure


Source ID Structure
AlphaFold DB D7RU03


Auxiliary protein

ID   2402 GenBank   WP_001749975
Name   WP_001749975_AR_0133|unnamed2 insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   5490 GenBank   WP_000342688
Name   t4cp2_AM380_RS21150_AR_0133|unnamed2 insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 26236..36561

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM380_RS20890 (AM380_20855) 21480..21686 - 207 WP_001749967 hypothetical protein -
AM380_RS20900 (AM380_20865) 21876..23375 + 1500 WP_004118622 IS21 family transposase -
AM380_RS20905 (AM380_20870) 23372..24127 + 756 WP_038423655 IS21-like element ISEc10 family helper ATPase IstB -
AM380_RS20910 (AM380_20875) 24176..24748 - 573 WP_108657686 restriction endonuclease -
AM380_RS20915 (AM380_20880) 24804..25115 - 312 WP_001452736 hypothetical protein -
AM380_RS20920 (AM380_20885) 25151..25465 - 315 WP_001749965 TrbM/KikA/MpfK family conjugal transfer protein -
AM380_RS20925 (AM380_20890) 25462..25806 - 345 WP_001749964 hypothetical protein -
AM380_RS20930 (AM380_20895) 25822..26127 - 306 WP_000960954 H-NS family nucleoid-associated regulatory protein -
AM380_RS20935 (AM380_20900) 26236..26970 + 735 WP_001749963 lytic transglycosylase domain-containing protein virB1
AM380_RS20940 (AM380_20905) 26979..27260 + 282 WP_000440698 transcriptional repressor KorA -
AM380_RS20945 (AM380_20910) 27270..27563 + 294 WP_001749962 hypothetical protein virB2
AM380_RS20950 (AM380_20915) 27613..27930 + 318 WP_000496058 VirB3 family type IV secretion system protein virB3
AM380_RS20955 (AM380_20920) 27930..30530 + 2601 WP_001749961 VirB4 family type IV secretion/conjugal transfer ATPase virb4
AM380_RS20960 (AM380_20925) 30497..31261 + 765 WP_013149461 type IV secretion system protein virB5
AM380_RS20965 (AM380_20930) 31269..31496 + 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
AM380_RS20970 (AM380_20935) 31512..32552 + 1041 WP_001749958 type IV secretion system protein virB6
AM380_RS21815 (AM380_20940) 32653..32781 + 129 WP_071882546 conjugal transfer protein TraN -
AM380_RS20980 (AM380_20945) 32771..33469 + 699 WP_000646594 type IV secretion system protein virB8
AM380_RS20985 (AM380_20950) 33480..34364 + 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
AM380_RS20990 (AM380_20955) 34364..35524 + 1161 WP_000101710 type IV secretion system protein VirB10 virB10
AM380_RS20995 (AM380_20960) 35566..36561 + 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
AM380_RS21000 (AM380_20965) 36561..37094 + 534 WP_000792636 phospholipase D family protein -
AM380_RS21820 37268..37555 - 288 Protein_49 DUF6710 family protein -
AM380_RS21010 (AM380_20975) 37822..38001 + 180 Protein_50 helix-turn-helix domain-containing protein -
AM380_RS21015 (AM380_20980) 38066..38770 + 705 WP_108657685 IS6-like element IS26 family transposase -
AM380_RS21025 (AM380_20990) 38816..39238 - 423 Protein_52 alpha/beta hydrolase-fold protein -
AM380_RS21030 (AM380_20995) 39260..40369 - 1110 WP_000842134 S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase -

Host bacterium

ID   8245 GenBank   NZ_CP028958
Plasmid name   AR_0133|unnamed2 Incompatibility group   IncN
Plasmid size   71304 bp Coordinate of oriT [Strand]   69616..69716 [+]
Host baterium   Morganella morganii strain AR_0133

Cargo genes

Drug resistance gene   dfrA14, tet(D), blaKPC-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -