Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107802
Name   oriT1_R485 in_silico
Organism   Morganella morganii
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NC_016036 (4558..4906 [-], 349 nt)
oriT length   349 nt
IRs (inverted repeats)      134..140, 154..160  (AAAATAT..ATATTTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 349 nt

>oriT1_R485
ACTGCGATGCAGGATAGGGCAAACGCCGCAAAATGACGTCTCTGACGCCATTCCGCAACGTTTGCCCGATGACCTACAGTCGGTTCTTGTTGGAGAGTTTTATGAAATATTTGGTCAAAGAGTTTATTAACGAAAAATATACTAAGGCTGTTAATATTTTAAAGGATAACCTTAAAGAACACTATCATGTTTTTTATTGTGTGAGATTAAGTGAGATTCTTTTTCCTGCCAGTGAATATGGAACTGAACAGTTTTTTAGTGATTTTGAAAAGATAAACTCCATTTCATTACCTGTCTTAGTGTTTGATCTCAAAGAGGGTGTTCCTGTGATTGTCATTAGTCTTGATGA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   5239 GenBank   WP_000539538
Name   mobP1_HXG63_RS00110_R485 insolico UniProt ID   A0A5J2A6K9
Length   388 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 388 a.a.        Molecular weight: 44507.83 Da        Isoelectric Point: 10.5067

>WP_000539538.1 MULTISPECIES: MobP1 family relaxase [Enterobacterales]
MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAVTRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKDHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKVKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDVGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GDLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFWSL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(70-229)


  Protein structure


Source ID Structure
AlphaFold DB A0A5J2A6K9


T4CP

ID   5483 GenBank   WP_014106755
Name   t4cp2_HXG63_RS00180_R485 insolico UniProt ID   _
Length   611 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 611 a.a.        Molecular weight: 69401.28 Da        Isoelectric Point: 7.1491

>WP_014106755.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacterales]
MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESNIRIDIRLTAIPS
LLSGMVSSLIVPVFIIWQLNKTDVALYGDAKFASDNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKSRGRSTSQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKVSPKLASLTMELNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(102-571)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 14086..25382

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXG63_RS00075 (R485_17) 9337..9618 + 282 WP_012845398 hypothetical protein -
HXG63_RS00080 (R485_18) 9608..9850 + 243 WP_000008708 hypothetical protein -
HXG63_RS00085 (R485_19) 9944..10273 + 330 WP_000866654 hypothetical protein -
HXG63_RS00410 (R485_20) 10316..10495 + 180 WP_024133577 hypothetical protein -
HXG63_RS00090 (R485_21) 10517..10711 + 195 WP_001714231 hypothetical protein -
HXG63_RS00095 (R485_22) 10728..11000 + 273 WP_000167280 hypothetical protein -
HXG63_RS00105 (R485_23) 11326..11871 + 546 WP_014106752 DNA distortion polypeptide 1 -
HXG63_RS00110 (R485_24) 11874..13040 + 1167 WP_000539538 MobP1 family relaxase -
HXG63_RS00115 (R485_25) 13393..13911 + 519 WP_001446885 transcription termination/antitermination NusG family protein -
HXG63_RS00120 13841..14053 + 213 WP_000125172 hypothetical protein -
HXG63_RS00125 (R485_26) 14086..14733 + 648 WP_000953526 lytic transglycosylase domain-containing protein virB1
HXG63_RS00130 (R485_27) 14711..15007 + 297 WP_000916182 TrbC/VirB2 family protein virB2
HXG63_RS00135 (R485_28) 15026..17779 + 2754 WP_014106753 type IV secretion system protein virb4
HXG63_RS00140 (R485_29) 17790..18548 + 759 WP_000744200 type IV secretion system protein -
HXG63_RS00145 (R485_30) 18549..18776 + 228 WP_000835349 EexN family lipoprotein -
HXG63_RS00150 (R485_31) 18786..19919 + 1134 WP_000513457 type IV secretion system protein virB6
HXG63_RS00155 (R485_33) 20164..20877 + 714 WP_000394615 type IV secretion system protein virB8
HXG63_RS00160 (R485_34) 20882..21811 + 930 WP_000783379 TrbG/VirB9 family P-type conjugative transfer protein virB9
HXG63_RS00170 (R485_37) 23120..24349 + 1230 WP_014106754 VirB10/TraB/TrbI family type IV secretion system protein virB10
HXG63_RS00175 (R485_38) 24351..25382 + 1032 WP_001058465 P-type DNA transfer ATPase VirB11 virB11
HXG63_RS00180 (R485_39) 25385..27220 + 1836 WP_014106755 type IV secretory system conjugative DNA transfer family protein -
HXG63_RS00185 (R485_40) 27217..27630 + 414 WP_000722603 cag pathogenicity island Cag12 family protein -
HXG63_RS00190 (R485_41) 27627..27929 + 303 WP_000717624 TrbM/KikA/MpfK family conjugal transfer protein -
HXG63_RS00195 (R485_42) 28044..29015 - 972 WP_247709884 SMEK domain-containing protein -
HXG63_RS00200 29057..29754 + 698 WP_247709885 IS1-like element IS1B family transposase -
HXG63_RS00205 (R485_45) 29925..30122 + 198 WP_014106757 hypothetical protein -

Host bacterium

ID   8237 GenBank   NC_016036
Plasmid name   R485 Incompatibility group   IncX1
Plasmid size   61093 bp Coordinate of oriT [Strand]   4558..4906 [-]; 42644..42709 [-]
Host baterium   Morganella morganii

Cargo genes

Drug resistance gene   sul2
Virulence gene   mrkA, mrkB, mrkC, mrkD, mrkF
Metal resistance gene   arsH
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -