Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107801
Name   oriT_pMR3-OXA181 in_silico
Organism   Morganella morganii strain MRSN22709
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KM660724 (43640..43740 [+], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      80..85, 91..96  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_pMR3-OXA181
TATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   5238 GenBank   WP_012561166
Name   mobF_HTD99_RS00240_pMR3-OXA181 insolico UniProt ID   D7RU03
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120141.30 Da        Isoelectric Point: 6.5229

>WP_012561166.1 MULTISPECIES: MobF family relaxase [Gammaproteobacteria]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure


Source ID Structure
AlphaFold DB D7RU03


Auxiliary protein

ID   776 GenBank   WP_001749975
Name   WP_001749975_pMR3-OXA181 insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   5482 GenBank   WP_000342688
Name   t4cp2_HTD99_RS00245_pMR3-OXA181 insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 10087..20412

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTD99_RS00040 5932..7365 - 1434 WP_001288432 DNA cytosine methyltransferase -
HTD99_RS00045 7464..7736 + 273 Protein_9 IS1 family transposase -
HTD99_RS00050 7747..7953 - 207 WP_001749967 hypothetical protein -
HTD99_RS00055 7958..8605 - 648 WP_015344958 restriction endonuclease -
HTD99_RS00060 8655..8888 - 234 WP_001191790 hypothetical protein -
HTD99_RS00065 9002..9316 - 315 WP_001749965 TrbM/KikA/MpfK family conjugal transfer protein -
HTD99_RS00070 9313..9657 - 345 WP_001749964 hypothetical protein -
HTD99_RS00075 9673..10044 - 372 WP_011867773 H-NS family nucleoid-associated regulatory protein -
HTD99_RS00080 10087..10821 + 735 WP_001749963 lytic transglycosylase domain-containing protein virB1
HTD99_RS00085 10830..11111 + 282 WP_000440698 transcriptional repressor KorA -
HTD99_RS00090 11121..11414 + 294 WP_001749962 hypothetical protein virB2
HTD99_RS00095 11464..11781 + 318 WP_036410084 VirB3 family type IV secretion system protein virB3
HTD99_RS00100 11781..14381 + 2601 WP_001749961 VirB4 family type IV secretion/conjugal transfer ATPase virb4
HTD99_RS00105 14399..15112 + 714 WP_001749960 type IV secretion system protein virB5
HTD99_RS00110 15120..15347 + 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
HTD99_RS00115 15363..16403 + 1041 WP_001749958 type IV secretion system protein virB6
HTD99_RS00120 16474..16632 + 159 WP_012561180 hypothetical protein -
HTD99_RS00125 16622..17320 + 699 WP_000646594 type IV secretion system protein virB8
HTD99_RS00130 17331..18215 + 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
HTD99_RS00135 18215..19375 + 1161 WP_000101710 type IV secretion system protein VirB10 virB10
HTD99_RS00140 19417..20412 + 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
HTD99_RS00145 20412..20945 + 534 WP_000792636 phospholipase D family protein -
HTD99_RS00375 21119..21406 - 288 Protein_30 DUF6710 family protein -
HTD99_RS00155 21673..21852 + 180 Protein_31 helix-turn-helix domain-containing protein -
HTD99_RS00160 21917..22621 + 705 WP_001067855 IS6-like element IS26 family transposase -
HTD99_RS00165 22675..23306 - 632 Protein_33 transposase -
HTD99_RS00170 23701..24357 + 657 WP_001516695 quinolone resistance pentapeptide repeat protein QnrS1 -
HTD99_RS00175 24676..25290 - 615 WP_014839983 recombinase family protein -

Host bacterium

ID   8236 GenBank   NZ_KM660724
Plasmid name   pMR3-OXA181 Incompatibility group   ColKP3
Plasmid size   57797 bp Coordinate of oriT [Strand]   43640..43740 [+]
Host baterium   Morganella morganii strain MRSN22709

Cargo genes

Drug resistance gene   dfrA14, qnrS1, blaOXA-181
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -