Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 107759
• Name: oriT_pJM2D
• Organism: Lactococcus cremoris strain JM2
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP121079 (6345..6481 [+], 137 nt)
• oriT length: 137 nt
• IRs (inverted repeats): 21..28, 42..49 (AAAAGGGG..CCCCTTTT) 25..30, 39..44 (GGGGAA..TTCCCC)
• Location of nic site: 104..105
• Conserved sequence flanking the nic site: GCTTGCAGTA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 137 nt

>oriT_pJM2D
CGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCAACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_LLJM2_RS13435_pJM2D

• ID: 5211
• GenBank: WP_281441155
• Name: Relaxase_LLJM2_RS13435_pJM2D
• UniProt ID: _
• Length: 240 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 240 a.a.
• Molecular weight: 27936.69 Da
• Isoelectric Point: 9.0825

>WP_281441155.1 relaxase/mobilization nuclease domain-containing protein [Lactococcus cremoris]
MTVIYMPKQSNGTVHSSKDLNQLIDYVMNPEKTNDFEYVSGQNILDIHSTCDEMLATRTMAIALKNKPQK
NEIYGYHFVQSFSPDDHLTPEQVHEIGLKTMKEYLGNSAEFIIATHTDKDHLHNHIVLNATNPLTLNKFQ
QNKNDLERLKEISDKISKEYGCKIIDRPEQKLGNSHKNYLVYLAQNSYRKEIKNKLEFLVSHSHTWEDFK
TKARALNLKVDDTKKIYHLSFRGKRANQKN

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (17-238)

Protein structure

No available structure.

Host bacterium

• ID: 8194
• GenBank: NZ_CP121079
• Plasmid name: pJM2D
• Incompatibility group: -
• Plasmid size: 19241 bp
• Coordinate of oriT [Strand]: 6345..6481 [+]
• Host baterium: Lactococcus cremoris strain JM2

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -